1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_10

Reclassification of as gen. nov., comb. nov. Free

Abstract

To reinvestigate the taxonomy of [] , 474 strains, mainly from mice and rats, were characterized by phenotype and 130 strains selected for genotypic characterization by 16S rRNA and partial gene sequencing. The type strain was further investigated by whole-genome sequencing. Phylogenetic analysis of the DNA sequences showed one monophyletic group with intragroup similarities of 96.7 and 97.2 % for the 16S rRNA and genes, respectively. The highest 16S rRNA gene sequence similarity to a taxon with a validly published name outside the group was 95.9 %, to the type strain of [Pasteurella] The closest related taxon based on sequence comparison was ‘’, with 88.4 % similarity. A new genus and a new combination, gen. nov., comb. nov., are proposed based on a distinct phylogenetic position based on 16S rRNA and gene sequence comparisons, with major divergence from the existing genera of the family . The new genus has the characteristics of with the emendation that acid formation from ( − )--mannitol and hydrolysis of aesculin are variable, while the α-glucosidase test is positive. There is no requirement for exogenously supplied NAD (V factor) for the majority of strains investigated; however, one strain was found to require NAD. The major fatty acids of the type strain of were C, C 3-OH/iso-C I, Cω7 and C, which is in line with most genera of the . The type strain of is CCUG 16938 ( = NCTC 12432 = ATCC 49577).

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000417
2015-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/10/3344.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000417&mimeType=html&fmt=ahah

References

  1. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. ( 1997;). Gapped blast psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25 3389–3402. [CrossRef]
    [Google Scholar]
  2. Angen Ø., Mutters R., Caugant D.A., Olsen J.E., Bisgaard M. ( 1999;). Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA–DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.. Int J Syst Bacteriol 49 67–86 [View Article] [PubMed].
     [Google Scholar]
  3. Angen Ø., Ahrens P., Kuhnert P., Christensen H., Mutters R. ( 2003;). Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis ‘Haemophilus somnus’, ‘Haemophilus agni’ and ‘Histophilus ovis’. Int J Syst Evol Microbiol 53 1449–1456 [View Article] [PubMed].
     [Google Scholar]
  4. Bisgaard M. ( 1986;). Actinobacillus muris sp. nov. isolated from mice. Acta Pathol Microbiol Immunol Scand [B] 94 1–8 [PubMed].
     [Google Scholar]
  5. Bisgaard M. ( 1993;). Ecology and significance of Pasteurellaceae in animals. Zentralbl Bakteriol 279 7–26 [View Article] [PubMed].
     [Google Scholar]
  6. Bisgaard M., Christensen H. ( 2012;). Classification of avian haemolytic Actinobacillus-like organisms (Bisgaard taxon 26) associated with anseriforme birds as Actinobacillus anseriformium sp. nov. Int J Syst Evol Microbiol 62 352–358 [View Article] [PubMed].
     [Google Scholar]
  7. Bisgaard M., Mutters R. ( 1986;). Characterization of some previously unclassified “Pasteurella” spp. obtained from the oral cavity of dogs and cats and description of a new species tentatively classified with the family Pasteurellaceae Pohl 1981 and provisionally called taxon 16. Acta Pathol Microbiol Immunol Scand [B] 94 177–184 [PubMed].
     [Google Scholar]
  8. Bisgaard M., Korczak B.M., Busse H.-J., Kuhnert P., Bojesen A.M., Christensen H. ( 2009;). Classification of the taxon 2 and taxon 3 complex of Bisgaard within Gallibacterium and description of Gallibacterium melopsittaci sp. nov., Gallibacterium trehalosifermentans sp. nov. and Gallibacterium salpingitidis sp. nov. Int J Syst Evol Microbiol 59 735–744 [View Article] [PubMed].
     [Google Scholar]
  9. Bisgaard M., Nørskov-Lauritsen N., de Wit S.J., Hess C., Christensen H. ( 2012;). Multilocus sequence phylogenetic analysis of Avibacterium . Microbiology 158 993–1004 [View Article] [PubMed].
     [Google Scholar]
  10. Blackall P.J., Christensen H., Beckenham T., Blackall L.L., Bisgaard M. ( 2005;). Reclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov.. Int J Syst Evol Microbiol 55 353–362 [View Article] [PubMed].
     [Google Scholar]
  11. Blackall P.J., Bojesen A.M., Christensen H., Bisgaard M. ( 2007;). Reclassification of [Pasteurella] trehalosi as Bibersteinia trehalosi gen. nov., comb. nov.. Int J Syst Evol Microbiol 57 666–674 [View Article] [PubMed].
     [Google Scholar]
  12. Brumpt E. ( 1910). Précis de Parasitologie Paris: Masson et Cie; (in French) .
     [Google Scholar]
  13. Christensen H., Bisgaard M. ( 2004;). Revised definition of Actinobacillus sensu stricto isolated from animals. A review with special emphasis on diagnosis. Vet Microbiol 99 13–30 [View Article] [PubMed].
     [Google Scholar]
  14. Christensen H., Bisgaard M. ( 2006;). The Genus Pasteurella . [View Article] In The Prokaryotes, vol. 6, , 3rd edn.., pp. 1062–1090. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer;.
     [Google Scholar]
  15. Christensen H., Bisgaard M., Angen O., Olsen J.E. ( 2002;). Final classification of Bisgaard taxon 9 as Actinobacillus arthritidis sp. nov. and recognition of a novel genomospecies for equine strains of Actinobacillus lignieresii . Int J Syst Evol Microbiol 52 1239–1246 [View Article] [PubMed].
     [Google Scholar]
  16. Christensen H., Bisgaard M., Aalbaek B., Olsen J.E. ( 2004a;). Reclassification of Bisgaard taxon 33, with proposal of Volucribacter psittacicida gen. nov., sp. nov. and Volucribacter amazonae sp. nov. as new members of the Pasteurellaceae . Int J Syst Evol Microbiol 54 813–818 [View Article] [PubMed].
     [Google Scholar]
  17. Christensen H., Kuhnert P., Olsen J.E., Bisgaard M. ( 2004b;). Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae . Int J Syst Evol Microbiol 54 1601–1609 [View Article] [PubMed].
     [Google Scholar]
  18. Christensen H., Kuhnert P., Busse H.-J., Frederiksen W.C., Bisgaard M. ( 2007;). Proposed minimal standards for the description of genera, species and subspecies of Pasteurellaceae . Int J Syst Evol Microbiol 57 166–178. [CrossRef]
    [Google Scholar]
  19. Christensen H., Korczak B.M., Bojesen A.M., Kuhnert P., Frederiksen W., Bisgaard M. ( 2011;). Classification of organisms previously reported as the SP and Stewart-Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group. Int J Syst Evol Microbiol 61 1829–1836 [View Article] [PubMed].
     [Google Scholar]
  20. Christensen H., Nicklas W., Bisgaard M. ( 2014;). Investigation of taxa of the family Pasteurellaceae isolated from Syrian and European hamsters and proposal of Mesocricetibacter intestinalis gen. nov., sp. nov. and Cricetibacter osteomyelitidis gen. nov., sp. nov. Int J Syst Evol Microbiol 64 3636–3643 [View Article] [PubMed].
     [Google Scholar]
  21. Dewhirst F.E., Paster B.J., Olsen I., Fraser G.J. ( 1993;). Phylogeny of the Pasteurellaceae as determined by comparison of 16S ribosomal ribonucleic acid sequences. Zentralbl Bakteriol 279 35–44 [View Article] [PubMed].
     [Google Scholar]
  22. Foster G., Ross H.M., Malnick H., Willems A., Hutson R.A., Reid R.J., Collins M.D. ( 2000;). Phocoenobacter uteri gen. nov., sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from a harbour porpoise (Phocoena phocoena). Int J Syst Evol Microbiol 50 135–139 [View Article] [PubMed].
     [Google Scholar]
  23. Foster G., Higgins R., Leclair D., Korczak B.M., Mikaelian I., Patterson I.A., Kuhnert P. ( 2011;). Proposal of Bisgaardia hudsonensis gen. nov., sp. nov. and an additional genomospecies, isolated from seals, as new members of the family Pasteurellaceae . Int J Syst Evol Microbiol 61 3016–3022 [View Article] [PubMed].
     [Google Scholar]
  24. Gregersen R.H., Neubauer C., Christensen H., Bojesen A.M., Hess M., Bisgaard M. ( 2009;). Comparative studies on [Pasteurella] testudinis and [P.] testudinis-like bacteria and proposal of Chelonobacter oris gen. nov., sp. nov. as a new member of the family Pasteurellaceae . Int J Syst Evol Microbiol 59 1583–1588 [View Article] [PubMed].
     [Google Scholar]
  25. Hall T.A. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 95–98.
     [Google Scholar]
  26. Hansen M.J., Bertelsen M.F., Christensen H., Bojesen A.M., Bisgaard M. ( 2012;). Otariodibacter oris gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of pinnipeds. Int J Syst Evol Microbiol 62 2572–2578 [View Article] [PubMed].
     [Google Scholar]
  27. Kilian M. ( 1976;). A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol 93 9–62 [View Article] [PubMed].
     [Google Scholar]
  28. Kilian M. ( 2005;). Genus III. Haemophilus Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1917, 561AL . . In Bergey's Manual of Systematic Bacteriology, vol. 2B, , 2nd edn.., pp. 883–904. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. New York: Springer;.
     [Google Scholar]
  29. Korczak B.M., Kuhnert P. ( 2008;). Phylogeny of Pasteurellaceae . . In Pasteurellaceae, Biology, Genomics and Molecular Aspects, pp. 27–52. Edited by Kuhnert P., Christensen H. Wymondham, UK: Caister Academic Press;.
     [Google Scholar]
  30. Korczak B., Christensen H., Emler S., Frey J., Kuhnert P. ( 2004;). Phylogeny of the family Pasteurellaceae based on rpoB sequences. Int J Syst Evol Microbiol 54 1393–1399 [View Article] [PubMed].
     [Google Scholar]
  31. Korczak B.M., Bisgaard M., Christensen H., Kuhnert P. ( 2014;). Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds. Antonie van Leeuwenhoek 105 731–741 [View Article] [PubMed].
     [Google Scholar]
  32. Kuhnert P., Korczak B., Falsen E., Straub R., Hoops A., Boerlin P., Frey J., Mutters R. ( 2004;). Nicoletella semolina gen. nov., sp. nov., a new member of Pasteurellaceae isolated from horses with airway disease. J Clin Microbiol 42 5542–5548 [View Article] [PubMed].
     [Google Scholar]
  33. Kuhnert P., Scholten E., Haefner S., Mayor D., Frey J. ( 2010;). Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen. Int J Syst Evol Microbiol 60 44–50 [View Article] [PubMed].
     [Google Scholar]
  34. Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., other authors. ( 2007;). Clustal W and Clustal X version 2.0. Bioinformatics 23 2947–2948 [View Article] [PubMed].
     [Google Scholar]
  35. Mühldorfer K., Speck S., Wibbelt G. ( 2014;). Proposal of Vespertiliibacter pulmonis gen. nov., sp. nov. and two genomospecies as new members of the family Pasteurellaceae isolated from European bats. Int J Syst Evol Microbiol 64 2424–2430 [View Article] [PubMed].
     [Google Scholar]
  36. Mutters R., Ihm P., Pohl S., Frederiksen W., Mannheim W. ( 1985;). Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa . Int J Syst Bacteriol 35 309–322 [View Article].
     [Google Scholar]
  37. Nicklas W. ( 2007;). Pasteurellaceae. [View Article] In The Mouse in Biomedical Research, vol. 2, , 2nd edn.., pp. 469–505. Edited by Fox J. G., Davisson M. T., Quimby F. W., Barthold S. W., Newcomer C. E., Smith A. L. Philadelphia: Elsevier;.
     [Google Scholar]
  38. Nørskov-Lauritsen N., Kilian M. ( 2006;). Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates. Int J Syst Evol Microbiol 56 2135–2146 [View Article] [PubMed].
     [Google Scholar]
  39. Nørskov-Lauritsen N., Bruun B., Kilian M. ( 2005;). Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov. Int J Syst Evol Microbiol 55 449–456 [View Article] [PubMed].
     [Google Scholar]
  40. Osawa R., Rainey F., Fujisawa T., Lang E., Busse H.-J., Walsh T.P., Stackebrandt E. ( 1995;). Lonepinella koalarum gen. nov., sp. nov., a new tannin-protein complex degrading bacterium. Syst Appl Microbiol 18 368–373 [View Article].
     [Google Scholar]
  41. Overbeek R., Olson R., Pusch G.D., Olsen G.J., Davis J.J., Disz T., Edwards R.A., Gerdes S., Parrello B., other authors. ( 2014;). The seed and the Rapid Annotation of microbial genomes using Subsystems Technology (rast). Nucleic Acids Res 42 (D1), D206–D214 [View Article] [PubMed].
     [Google Scholar]
  42. Piechulla K., Bisgaard M., Gerlach H., Mannheim W. ( 1985;). Taxonomy of some recently described avian Pasteurella/Actinobacillus-like organisms as indicated by deoxyribonucleic acid relatedness. Avian Pathol 14 281–311 [View Article] [PubMed].
     [Google Scholar]
  43. Rice P., Longden I., Bleasby A. ( 2000;). emboss: the European Molecular Biology Open Software Suite. Trends Genet 16 276–277 [View Article] [PubMed].
     [Google Scholar]
  44. Ryll M., Mutters R., Mannheim W. ( 1991;). Untersuchungen zur genetischen Klassifikation des Pasteurella-pneumotropica-Komplexes. Berl Munch Tierarztl Wochenschr 104 243–245 (in German). [PubMed].
     [Google Scholar]
  45. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846–849 [View Article].
     [Google Scholar]
  46. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  47. Trevisan V. ( 1887;). Sul Micrococco della rabbia e sulla possiblità di riconoscere durante il periodo d'incubazione, dall'esame del sangue della persona moricata, se ha contratta l'infezione rabbica. Rend Ist Lomb Sci Lett Ser 2 20 88–105 (in Italian) .
     [Google Scholar]
  48. Winslow C.E., Broadhurst J., Buchanan R.E., Krumwiede C., Rogers L.A., Smith G.H. ( 1917;). The families and genera of the bacteria: preliminary report of the Committee of the Society of American Bacteriologists on characterization and classification of bacterial types. J Bacteriol 2 505–566 [PubMed].
     [Google Scholar]
  49. Zinnemann K., Biberstein E.L. ( 1974;). Genus Haemophilus Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1917, 561. . In Bergey's Manual of Determinative Bacteriology , 8th edn.., pp. 364–370. Edited by Buchanan R. E., Gibbons N. E. Baltimore: Williams & Wilkins;.
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000417
Loading
Reclassification of Actinobacillus muris as Muribacter muris gen. nov., comb. nov.
Int J Syst Evol Microbiol 65, 3344 (2015); https://doi.org/10.1099/ijsem.0.000417
/content/journal/ijsem/10.1099/ijsem.0.000417
/content/journal/ijsem/10.1099/ijsem.0.000417
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error