A Gram-stain-positive, aerobic, straight or slightly bent rod-shaped, non-motile, non-spore-forming bacterium, designated strain CC5-806T, was isolated from a soil sample collected from a wild karst cave in the Wulong region, Chongqing, PR China and examined using a polyphasic approach to clarify its taxonomic position. This bacterium did not produce substrate mycelium or aerial hyphae, and no diffusible pigments were observed on the media tested. Strain CC5-806T grew optimally without NaCl at 20 °C and at pH 7.0. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain CC5-806T belonged to the family Microbacteriaceae and showed the highest levels of 16S rRNA gene sequence similarities with Frigoribacterium endophyticum EGI 6500707T (97.56 %), Frigoribacterium faeni 801T (97.53 %) and Glaciihabitans tibetensis MP203T (97.42 %). Phylogenetic trees revealed that strain CC5-806T did not show a clear affiliation to any genus within the family Microbacteriaceae. The DNA G+C content of strain CC5-806T was 62.6 mol%. The cell-wall peptidoglycan contained l-lysine as a diagnostic diamino acid. The predominant menaquinones were MK-11, MK-10 and MK-9. Phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid, four unidentified phospholipids and other polar lipids were detected in the polar lipid extracts. The major fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C14 : 0. On the basis of the phylogenetic analysis, and phenotypic and chemotaxonomic characteristics, strain CC5-806T was distinguishable from phylogenetically related genera in the family Microbacteriaceae. It represents a novel species of a novel genus, for which the name Lysinibacter cavernae gen. nov., sp. nov. is proposed. The type strain is CC5-806T ( = DSM 27960T = CGMCC 1.14983T).
CardinaleM.,
GrubeM.,
BergG.
( 2011;). Frondihabitans cladoniiphilus sp. nov., an actinobacterium of the family Microbacteriaceae isolated from lichen, and emended description of the genus Frondihabitans
. Int J Syst Evol Microbiol613033–3038 [View Article][PubMed].
ChunJ.,
LeeJ.-H.,
JungY.,
KimM.,
KimS.,
KimB.K.,
LimY.-W.
( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol572259–2261 [View Article][PubMed].
CollinsM.D.,
PirouzT.,
GoodfellowM.,
MinnikinD.E.
( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100221–230 [View Article][PubMed].
EuzébyJ.P.
( 1997;). List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol47590–592 [View Article][PubMed].
GreeneA.C.,
EuzébyJ.P.,
TindallB.J.,
PatelB.K.
( 2009;). Proposal of Frondihabitans gen. nov. to replace the illegitimate genus name Frondicola Zhang et al. 2007. Int J Syst Evol Microbiol59447–448.
[PubMed]. [CrossRef]
GuoL.,
TuoL.,
HabdenX.,
ZhangY.,
LiuJ.,
JiangZ.,
LiuS.,
DilbarT.,
SunC.
( 2015;). Allosalinactinospora lopnorensis gen. nov., sp. nov., a new member of the family Nocardiopsaceae isolated from soil. Int J Syst Evol Microbiol65206–213 [View Article][PubMed].
JangY.-H.,
KimS.-J.,
HamadaM.,
TamuraT.,
AhnJ.-H.,
WeonH.-Y.,
SuzukiK.,
KwonS.-W.
( 2012;). Diaminobutyricimonas aerilata gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from an air sample in Korea. J Microbiol501047–1052 [View Article][PubMed].
JangG.I.,
ChoY.,
ChoB.C.
( 2013a;). Pontimonas salivibrio gen. nov., sp. nov., a new member of the family Microbacteriaceae isolated from a seawater reservoir of a solar saltern. Int J Syst Evol Microbiol632124–2131 [View Article][PubMed].
KämpferP.,
RaineyF.A.,
AnderssonM.A.,
Nurmiaho LassilaE.-L.,
UlrychU.,
BusseH.-J.,
WeissN.,
MikkolaR.,
Salkinoja-SalonenM.
( 2000;). Frigoribacterium faeni gen. nov., sp. nov., a novel psychrophilic genus of the family Microbacteriaceae
. Int J Syst Evol Microbiol50355–363 [View Article][PubMed].
KellyK.L.
( 1964). Inter-Society Color Council-National Bureau of Standards Color name Charts illustrated with Centroid Colors
Washington, DC:
US Government Printing Office;.
KimH.,
ParkD.-S.,
OhH.-W.,
LeeK.H.,
ChungD.-H.,
ParkH.-Y.,
ParkH.-M.,
BaeK.S.
( 2012a;). Gryllotalpicola gen. nov., with descriptions of Gryllotalpicola koreensis sp. nov., Gryllotalpicola daejeonensis sp. nov. and Gryllotalpicola kribbensis sp. nov. from the gut of the African mole cricket, Gryllotalpa africana, and reclassification of Curtobacterium ginsengisoli as Gryllotalpicola ginsengisoli comb. nov. Int J Syst Evol Microbiol622363–2370 [View Article][PubMed].
KimS.-J.,
JangY.H.,
HamadaM.,
TamuraT.,
AhnJ.-H.,
WeonH.-Y.,
SuzukiK.,
KwonS.-W.
( 2012b;). Homoserinimonas aerilata gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from an air sample in Korea. J Microbiol50673–679 [View Article][PubMed].
KimuraM.
( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16111–120 [View Article][PubMed].
LeeS.D.
( 2010;). Frondihabitans peucedani sp. nov., an actinobacterium isolated from rhizosphere soil, and emended description of the genus Frondihabitans Greene et al. 2009. Int J Syst Evol Microbiol601740–1744 [View Article][PubMed].
LiW.-J.,
XuP.,
SchumannP.,
ZhangY.-Q.,
PukallR.,
XuL.-H.,
StackebrandtE.,
JiangC.-L.
( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia
. Int J Syst Evol Microbiol571424–1428 [View Article][PubMed].
LiH.-R.,
YuY.,
LuoW.,
ZengY.-X.
( 2010;). Marisediminicola antarctica gen. nov., sp. nov., an actinobacterium isolated from the Antarctic. Int J Syst Evol Microbiol602535–2539 [View Article][PubMed].
LiA.-H.,
LiuH.-C.,
XinY.-H.,
KimS.-G.,
ZhouY.-G.
( 2014;). Glaciihabitans tibetensis gen. nov., sp. nov., a psychrotolerant bacterium of the family Microbacteriaceae, isolated from glacier ice water. Int J Syst Evol Microbiol64579–587 [View Article][PubMed].
MageeC.M.,
RodeheaverG.,
EdgertonM.T.,
EdlichR.F.
( 1975;). A more reliable gram staining technic for diagnosis of surgical infections. Am J Surg130341–346 [View Article][PubMed].
MesbahM.,
PremachandranU.,
WhitmanW.B.
( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39159–167 [View Article].
SchumannP.,
ZhangD.-C.,
RedzicM.,
MargesinR.
( 2012;). Alpinimonas psychrophila gen. nov., sp. nov., an actinobacterium of the family Microbacteriaceae isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol622724–2730 [View Article][PubMed].
StackebrandtE.,
RaineyF.A.,
Ward-RaineyN.L.
( 1997;). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol47479–491 [View Article].
WaksmanS.A.
( 1961). Classification, Identification and Description of Genera and Species, vol. 2, The Actinomycetes
Baltimore, MD:
Williams & Wilkins;.
WeonH.-Y.,
KimS.-J.,
JangY.-H.,
HamadaM.,
TamuraT.,
AhnJ.-H.,
SuzukiK.,
KwonS.-W.
( 2013;). Naasia aerilata gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from air. Int J Syst Evol Microbiol632436–2441 [View Article][PubMed].
XuP.,
LiW.-J.,
TangS.-K.,
ZhangY.-Q.,
ChenG.-Z.,
ChenH.-H.,
XuL.-H.,
JiangC.-L.
( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol551149–1153 [View Article][PubMed].
ZhiX.-Y.,
LiW.-J.,
StackebrandtE.
( 2009;). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol59589–608.
[PubMed]. [CrossRef]