1887

Abstract

Strain T13, isolated from forest soil in Jeollabuk-do, South Korea, exhibited antibiotic production on yeast extract-malt extract-glucose (YMG) medium containing magnesium chloride as a trace mineral, and inhibited the growth of , , , , , , and . Growth occurred at 15–45 °C, pH 4–11 and in the presence of up to 2 % (w/v) NaCl. Biochemical analyses indicated that the predominant menaquinones produced by this strain were MK-9(H) and MK-9(H); small amounts of MK-10(H) and MK-10(H) were also detected. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, and the cell-wall peptidoglycan contained -diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates contained glucose, galactose, ribose and rhamnose. The fatty-acid profile of strain T13 was made up predominantly of iso- and anteiso-branched fatty acids. Genetic analyses demonstrated that strain T13 is closely related to JR-43 (98.29 % 16S rRNA gene sequence similarity), JR-19 (97.99 %), JR-41 (97.86 %), LMG 20096 (97.84 %), JL-22 (97.79 %) and Z4 (97.56 %), and DNA–DNA hybridization yielded relatedness values of 35.27–43.42 % when T13 was compared to related strains. The results of morphological, chemotaxonomic, phylogenetic and phenotypic analyses confirm that this strain represents a novel species of the genus , for which the name Streptomyces sp. nov. is proposed. The type strain is T13 ( = KEMB 9005-210 = KACC 18227 = NBRC 110901).

Funding
This study was supported by the:
  • Ministry of Agriculture, Food, and Rural Affairs (Award 312027-3)
  • Ministry of Education, Science, and Technology (Award 2011-0010144)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000404
2015-10-01
2021-04-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/10/3262.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000404&mimeType=html&fmt=ahah

References

  1. Anderson A.S., Wellington E.M.H. ( 2001;). The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51 797814 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bérdy J. ( 2005;). Bioactive microbial metabolites. J Antibiot (Tokyo) 58 126 [CrossRef] [PubMed].
    [Google Scholar]
  3. Clardy J., Fischbach M.A., Walsh C.T. ( 2006;). New antibiotics from bacterial natural products. Nat Biotechnol 24 15411550 [CrossRef] [PubMed].
    [Google Scholar]
  4. Coates A.R.M., Hu Y. ( 2007;). Novel approaches to developing new antibiotics for bacterial infections. Br J Pharmacol 152 11471154 [CrossRef] [PubMed].
    [Google Scholar]
  5. Collins M.D. ( 1985;). Analysis of isoprenoid quinones. Methods Microbiol 18 329366 [CrossRef].
    [Google Scholar]
  6. Cui X.L., Mao P.H., Zeng M., Li W.J., Zhang L.P., Xu L.H., Jiang C.L. ( 2001;). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51 357363. [CrossRef]
    [Google Scholar]
  7. Cundliffe E. ( 1989;). How antibiotic-producing organisms avoid suicide. Annu Rev Microbiol 43 207233 [CrossRef] [PubMed].
    [Google Scholar]
  8. Donadio S., Maffioli S., Monciardini P., Sosio M., Jabes D. ( 2010;). Antibiotic discovery in the twenty-first century: current trends and future perspectives. J Antibiot (Tokyo) 63 423430 [CrossRef] [PubMed].
    [Google Scholar]
  9. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [CrossRef].
    [Google Scholar]
  10. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [CrossRef].
    [Google Scholar]
  11. Frank J.A., Reich C.I., Sharma S., Weisbaum J.S., Wilson B.A., Olsen G.J. ( 2008;). Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 74 24612470 [CrossRef] [PubMed].
    [Google Scholar]
  12. Goodfellow M., Fiedler H.P. ( 2010;). A guide to successful bioprospecting: informed by actinobacterial systematics. Antonie van Leeuwenhoek 98 119142 [CrossRef] [PubMed].
    [Google Scholar]
  13. Hall T.A. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 9598.
    [Google Scholar]
  14. Hayakawa M., Nonomura H. ( 1987;). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65 501509 [CrossRef].
    [Google Scholar]
  15. Jacin H., Mishkin A.R. ( 1965;). Separation of carbohydrates on borate-impregnated silica gel G plates. J Chromatogr A 18 170173 [CrossRef] [PubMed].
    [Google Scholar]
  16. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [CrossRef] [PubMed].
    [Google Scholar]
  17. Kimura M. ( 1983). The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef].
    [Google Scholar]
  18. Klevens R.M., Edwards J.R., Tenover F.C., McDonald L.C., Horan T., Gaynes R., National Nosocomial Infections Surveillance System. ( 2006;). Changes in the epidemiology of methicillin-resistant Staphylococcus aureus in intensive care units in US hospitals, 1992-2003. Clin Infect Dis 42 389391 [CrossRef] [PubMed].
    [Google Scholar]
  19. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161207 [CrossRef].
    [Google Scholar]
  20. Kroppenstedt R.M. ( 1992;). The genus Nocardiopsis . . In The Prokaryotes vol. 2, 2nd edn, pp. 11391156. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;.
    [Google Scholar]
  21. Küster E., Williams S.T. ( 1964;). Selection of media for isolation of streptomycetes. Nature 202 928929 [CrossRef] [PubMed].
    [Google Scholar]
  22. Lechevalier M.P., Lechevalier H. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20 435443. [CrossRef]
    [Google Scholar]
  23. Lechevalier M.P., Lechevalier H.A. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Society for Industrial Microbiology Special publication no. 6, pp. 227291. Edited by Dietz A., Thayer J. Arlington, VA: Society for Industrial Microbiology;.
    [Google Scholar]
  24. Lechevalier M.P., De Bièvre C., Lechevalier H. ( 1977;). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5 249260 [CrossRef].
    [Google Scholar]
  25. Lee H.J., Whang K.S. ( 2014a;). Streptomyces graminisoli sp. nov. and Streptomyces rhizophilus sp. nov., isolated from bamboo (Sasa borealis) rhizosphere soil. Int J Syst Evol Microbiol 64 15461551 [CrossRef] [PubMed].
    [Google Scholar]
  26. Lee H.J., Whang K.S. ( 2014b;). Streptomyces graminifolii sp. nov., isolated from bamboo (Sasa borealis) litter. Int J Syst Evol Microbiol 64 25172521 [CrossRef] [PubMed].
    [Google Scholar]
  27. Lee H.J., Han S.I., Whang K.S. ( 2012;). Streptomyces gramineus sp. nov., an antibiotic-producing actinobacterium isolated from bamboo (Sasa borealis) rhizosphere soil. Int J Syst Evol Microbiol 62 856859 [CrossRef] [PubMed].
    [Google Scholar]
  28. Lewis K. ( 2013;). Platforms for antibiotic discovery. Nat Rev Drug Discov 12 371387 [CrossRef] [PubMed].
    [Google Scholar]
  29. Madigan M.T., Martinko J.M., Dunlap P.V., Clark D.P. ( 2009;). Metabolic diversity and microbial ecology. . In Brock Biology of Microorganisms , 12th edn.., pp. 805806 San Francisco: Benjamin Cummings;.
    [Google Scholar]
  30. Manfio G.P., Zakrzewska-Czerwinska J., Atalan E., Goodfellow M. ( 1995;). Towards minimal standards for the description of Streptomyces species. Biotekhnologiia 7 242283.
    [Google Scholar]
  31. Meier-Kolthoff J.P., Göker M., Spröer C., Klenk H.P. ( 2013;). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195 413418 [CrossRef] [PubMed].
    [Google Scholar]
  32. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167 [CrossRef].
    [Google Scholar]
  33. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [CrossRef].
    [Google Scholar]
  34. Moran G.J., Krishnadasan A., Gorwitz R.J., Fosheim G.E., McDougal L.K., Carey R.B., Talan D.A., EMERGEncy ID Net Study Group. ( 2006;). Methicillin-resistant S. aureus infections among patients in the emergency department. N Engl J Med 355 666674 [CrossRef] [PubMed].
    [Google Scholar]
  35. Newman D.J., Cragg G.M. ( 2012;). Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod 75 311335 [CrossRef] [PubMed].
    [Google Scholar]
  36. Pham V.H.T., Kim J. ( 2012;). Cultivation of unculturable soil bacteria. Trends Biotechnol 30 475484 [CrossRef] [PubMed].
    [Google Scholar]
  37. Prosser B.L.T., Palleroni N.J. ( 1976;). Streptomyces longwoodensis sp. nov. Int J Syst Bacteriol 26 319322 [CrossRef].
    [Google Scholar]
  38. Rohde M. ( 2011;). Microscopy. . In Methods in microbiology vol. 38, , 1st edn., pp. 61100 Edited by Rainey F., Oren A. Amsterdam: Academic Press, Elsevier;.
    [Google Scholar]
  39. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI Inca;.
  40. Shirling E.B., Gottlieb D. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16 313340 [CrossRef].
    [Google Scholar]
  41. Staneck J.L., Roberts G.D. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28 28 226231.
    [Google Scholar]
  42. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [CrossRef] [PubMed].
    [Google Scholar]
  43. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882. [CrossRef]
    [Google Scholar]
  44. Tindall B.J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60 [CrossRef] [PubMed].
    [Google Scholar]
  45. Torsvik V., Goksøyr J., Daae F.L. ( 1990;). High diversity in DNA of soil bacteria. Appl Environ Microbiol 56 782787.
    [Google Scholar]
  46. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [CrossRef].
    [Google Scholar]
  47. Williams S.T., Wellington E.M.H. ( 1982;). Actinomycetes. . In Methods of Soil Analysis, Part 2. Chemical and Biological Properties , 2nd edn.., pp. 969987. Edited by Page A. L., Miller R. H., Keeney D. R. Madison, WI: American Society for Agronomy & Soil Science Society of America;.
    [Google Scholar]
  48. Williams S.T., Goodfellow M., Alderson G. ( 1989;). Genus Streptomyces Waksman and Henrici 1943, 339AL . . In Bergey's Manual of Systematic Bacteriology, 4, pp. 24522492. Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;.
    [Google Scholar]
  49. Yamamura H., Ashizawa H., Hamada M., Hosoyama A., Komaki H., Otoguro M., Tamura T., Hayashi Y., Nakagawa Y., other authors. ( 2014;). Streptomyces hokutonensis sp. nov., a novel actinomycete isolated from the strawberry root rhizosphere. J Antibiot (Tokyo) 67 465470 [CrossRef] [PubMed].
    [Google Scholar]
  50. Zheng J., Zhang X., Xin Y., Han X., Ni S., Zhang J. ( 2013;). Streptomyces yaanensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 63 47194723 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000404
Loading
/content/journal/ijsem/10.1099/ijsem.0.000404
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error