1887

Abstract

Strain T13, isolated from forest soil in Jeollabuk-do, South Korea, exhibited antibiotic production on yeast extract-malt extract-glucose (YMG) medium containing magnesium chloride as a trace mineral, and inhibited the growth of , , , , , , and . Growth occurred at 15–45 °C, pH 4–11 and in the presence of up to 2 % (w/v) NaCl. Biochemical analyses indicated that the predominant menaquinones produced by this strain were MK-9(H) and MK-9(H); small amounts of MK-10(H) and MK-10(H) were also detected. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, and the cell-wall peptidoglycan contained -diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates contained glucose, galactose, ribose and rhamnose. The fatty-acid profile of strain T13 was made up predominantly of iso- and anteiso-branched fatty acids. Genetic analyses demonstrated that strain T13 is closely related to JR-43 (98.29 % 16S rRNA gene sequence similarity), JR-19 (97.99 %), JR-41 (97.86 %), LMG 20096 (97.84 %), JL-22 (97.79 %) and Z4 (97.56 %), and DNA–DNA hybridization yielded relatedness values of 35.27–43.42 % when T13 was compared to related strains. The results of morphological, chemotaxonomic, phylogenetic and phenotypic analyses confirm that this strain represents a novel species of the genus , for which the name Streptomyces sp. nov. is proposed. The type strain is T13 ( = KEMB 9005-210 = KACC 18227 = NBRC 110901).

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2015-10-01
2019-10-14
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