1887

Abstract

Two novel Gram-stain-negative, slow-growing, halotolerant strains with rod-shaped cells, designated as strains Mi-7 and Mi-8, which formed pin-point colonies on halophilic media were isolated during a study into the microbial diversity of a salt pan in the state of Tamilnadu, India. Both the strains had an obligate requirement for 1 % (w/v) NaCl for growth and were halotolerant, growing at NaCl concentrations of up to 20 % (w/v) in media. The strains, however, showed an inability to utilize the majority of substrates tested as sole carbon sources for growth and in fermentation reactions. Molecular phylogenetic analyses, based on 16S rRNA gene sequence revealed their closest phylogenetic neighbours to be members of the genus , with whom they showed the highest sequence similarity of 93.6 % and even less with the type strain of the type species, DSM 8798 (91.1 %). Similarities with other genera within the family were below 91.0 %. However, the two strains were very closely related to each other with 99.9 % sequence similarity, and DNA–DNA hybridization analyses confirmed their placement in the same species. The DNA G+C content of both strains was 65 mol%. Using the polyphasic taxonomic data obtained from this study, strains Mi-7 and Mi-8 represent two strains of the same species of a novel genus for which the name gen. nov., sp. nov., is proposed; the type strain of the novel species is Mi-7 ( = MTCC 12009 = DSM 28688).

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2015-10-01
2019-10-22
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