1887

Abstract

Two bacterial strains, designated MA1002 and MA1003, were isolated from the air-conditioning system of a car. Cells of both strains were Gram-reaction-positive, non-motile, non-spore-forming coccoids, catalase- and oxidase-positive and UV-radiation resistant. The major fatty acids of strain MA1002 were iso-C and iso-C and those of strain MA1003 were iso-C and iso-C H. The polar lipid profile of MA1002 contained phosphatidylethanolamine, two unidentified phosphoglycolipids, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid and an unidentified lipid. MA1003 had three unidentified phosphoglycolipids, six unidentified phospholipids, two unidentified glycolipids and two unidentified polar lipids as the polar lipids. The G+C contents of the genomic DNA of MA1002 and MA1003 were 70.5 and 76.0 mol%, respectively. MK-8 was the predominant respiratory quinone for both strains. 16S rRNA gene sequence analysis showed that strain MA1002 was phylogenetically related to DSM 19763, DSM 11300, TR0125 and ST0316 (92.9, 92.6, 92.0 and 91.9 % sequence similarity, respectively), and MA1003 showed the highest sequence similarity to KR-140 (92.9 %) and X-82 (91.4 %). The results of genotypic and phenotypic characterizations showed that both strains could be distinguished from phylogenetically related species, and that the strains represented novel species within the genus , for which we propose the names sp. nov. (type strain MA1002 = KACC 17964 = NBRC 110141) and sp. nov. (type strain is MA1003 = KACC 17965 = NBRC 110142).

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2015-09-01
2019-10-18
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