1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_9

sp. nov. and sp. nov., isolated from a car air-conditioning system Free

Abstract

Two bacterial strains, designated MA1002 and MA1003, were isolated from the air-conditioning system of a car. Cells of both strains were Gram-reaction-positive, non-motile, non-spore-forming coccoids, catalase- and oxidase-positive and UV-radiation resistant. The major fatty acids of strain MA1002 were iso-C and iso-C and those of strain MA1003 were iso-C and iso-C H. The polar lipid profile of MA1002 contained phosphatidylethanolamine, two unidentified phosphoglycolipids, an unidentified phospholipid, an unidentified aminophospholipid, an unidentified aminolipid and an unidentified lipid. MA1003 had three unidentified phosphoglycolipids, six unidentified phospholipids, two unidentified glycolipids and two unidentified polar lipids as the polar lipids. The G+C contents of the genomic DNA of MA1002 and MA1003 were 70.5 and 76.0 mol%, respectively. MK-8 was the predominant respiratory quinone for both strains. 16S rRNA gene sequence analysis showed that strain MA1002 was phylogenetically related to DSM 19763, DSM 11300, TR0125 and ST0316 (92.9, 92.6, 92.0 and 91.9 % sequence similarity, respectively), and MA1003 showed the highest sequence similarity to KR-140 (92.9 %) and X-82 (91.4 %). The results of genotypic and phenotypic characterizations showed that both strains could be distinguished from phylogenetically related species, and that the strains represented novel species within the genus , for which we propose the names sp. nov. (type strain MA1002 = KACC 17964 = NBRC 110141) and sp. nov. (type strain is MA1003 = KACC 17965 = NBRC 110142).

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000396
2015-09-01
2023-03-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/9/3175.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000396&mimeType=html&fmt=ahah

References

  1. Ahmed I., Abbas S., Kudo T., Iqbal M., Fujiwara T., Ohkuma M. ( 2014;). Deinococcus citri sp. nov., isolated from citrus leaf canker lesions. Int J Syst Evol Microbiol 64 4134–4140 [View Article] [PubMed] .
     [Google Scholar]
  2. Arrage A.A., Phelps T.J., Benoit R.E., Palumbo A.V., White D.C. ( 1993;). Bacterial sensitivity to UV light as a model for ionizing radiation resistance. J Microbiol Methods 18 127–136 [View Article].
     [Google Scholar]
  3. Asker D., Awad T.S., Beppu T., Ueda K. ( 2008;). Deinococcus misasensis and Deinococcus roseus, novel members of the genus Deinococcus, isolated from a radioactive site in Japan. Syst Appl Microbiol 31 43–49 [View Article] [PubMed] .
     [Google Scholar]
  4. Brooks B.W., Murray R.G.E. ( 1981;). Nomenclature for Micrococcus radiodurans and other radiation-resistant cocci: Deinococcacae fam. nov. and Deinococcus gen. nov., including five species. Int J Syst Bacteriol 31 353–360 [View Article].
     [Google Scholar]
  5. Counsell T.J., Murray R.G.E. ( 1986;). Polar lipid profiles of the genus Deinococcus . Int J Syst Bacteriol 36 202–206 [View Article].
     [Google Scholar]
  6. Dong N., Li H.R., Yuan M., Zhang X.H., Yu Y. ( 2015;). Deinococcus antarcticus sp. nov., isolated from soil. Int J Syst Evol Microbiol 65 331–335 [View Article] [PubMed] .
     [Google Scholar]
  7. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  8. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  9. Gonzalez J.M., Saiz-Jimenez C. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4 770–773 [View Article] [PubMed] .
     [Google Scholar]
  10. Kim D.U., Ka J.O. ( 2014;). Roseomonas soli sp. nov., isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Int J Syst Evol Microbiol 64 1024–1029 [View Article] [PubMed] .
     [Google Scholar]
  11. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., Other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed] .
     [Google Scholar]
  12. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161–207. [CrossRef]
    [Google Scholar]
  13. Lai W.-A., Kämpfer P., Arun A.B., Shen F.-T., Huber B., Rekha P.D., Young C.-C. ( 2006;). Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. Int J Syst Evol Microbiol 56 787–791 [View Article] [PubMed] .
     [Google Scholar]
  14. MIDI ( 1999). Sherlock Microbial Identification System, Operating Manual, version 3.0 Newark, DE: MIDI Inc;.
     [Google Scholar]
  15. Peng F., Zhang L., Luo X., Dai J., An H., Tang Y., Fang C. ( 2009;). Deinococcus xinjiangensis sp. nov., isolated from desert soil. Int J Syst Evol Microbiol 59 709–713 [View Article] [PubMed] .
     [Google Scholar]
  16. Rainey F.A., Nobre M.F., Schumann P., Stackebrandt E., da Costa M.S. ( 1997;). Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison. Int J Syst Bacteriol 47 510–514 [View Article] [PubMed] .
     [Google Scholar]
  17. Rainey F.A., Ray K., Ferreira M., Gatz B.Z., Nobre M.F., Bagaley D., Rash B.A., Park M.J., Earl A.M., Other authors. ( 2005;). Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. Appl Environ Microbiol 71 5225–5235 [View Article] [PubMed] .
     [Google Scholar]
  18. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  19. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  20. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 2725–2729 [View Article] [PubMed] .
     [Google Scholar]
  21. Thompson J.D., Higgins D.G., Gibson T.J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 4673–4680 [View Article] [PubMed] .
     [Google Scholar]
  22. Vaishampayan P., Roberts A.H., Augustus A., Pukall R., Schumann P., Schwendner P., Mayilraj S., Salmassi T., Venkateswaran K. ( 2014;). Deinococcus phoenicis sp. nov., an extreme ionizing-radiation-resistant bacterium isolated from the Phoenix Lander assembly facility. Int J Syst Evol Microbiol 64 3441–3446 [View Article] [PubMed] .
     [Google Scholar]
  23. Weon H.Y., Kim B.Y., Schumann P., Son J.A., Jang J., Go S.J., Kwon S.W. ( 2007;). Deinococcus cellulosilyticus sp. nov., isolated from air. Int J Syst Evol Microbiol 57 1685–1688 [View Article] [PubMed] .
     [Google Scholar]
  24. Yang Y., Itoh T., Yokobori S., Shimada H., Itahashi S., Satoh K., Ohba H., Narumi I., Yamagishi A. ( 2010;). Deinococcus aetherius sp. nov., isolated from the stratosphere. Int J Syst Evol Microbiol 60 776–779 [View Article] [PubMed] .
     [Google Scholar]
  25. Yoo S.H., Weon H.Y., Kim S.J., Kim Y.S., Kim B.Y., Kwon S.W. ( 2010;). Deinococcus aerolatus sp. nov. and Deinococcus aerophilus sp. nov., isolated from air samples. Int J Syst Evol Microbiol 60 1191–1195 [View Article] [PubMed] .
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000396
Loading
Deinococcus metallilatus sp. nov. and Deinococcus carri sp. nov., isolated from a car air-conditioning system
Int J Syst Evol Microbiol 65, 3175 (2015); https://doi.org/10.1099/ijsem.0.000396
/content/journal/ijsem/10.1099/ijsem.0.000396
/content/journal/ijsem/10.1099/ijsem.0.000396
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error