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Abstract

A novel halotolerant actinomycete, strain Chem15, was isolated from soil around Inche-Broun hypersaline wetland; its taxonomic position was determined based on a polyphasic approach. Strain Chem15 was strictly aerobic and tolerated NaCl up to 12.5 %. The optimum temperature and pH for growth were 28–30 °C and pH 7.0–7.5, respectively. The cell wall of strain Chem15 contained -diaminopimelic acid as diamino acid and galactose, arabinose and ribose as whole-cell sugars. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The cellular fatty acids profile consisted of C, iso-C, C 10-methyl and Cω9, and the major respiratory quinone was MK-8(H). The G+C content of the genomic DNA was 68.0 mol%. The novel strain constituted a distinct phyletic line within the genus , based on 16S rRNA gene sequence analysis, and was closely associated with DSM 45714 and DSM 44684 (98.2 and 98.1 % 16S rRNA gene sequence similarity, respectively). However DNA–DNA relatedness and phenotypic data demonstrated that strain Chem15 was clearly different from closely related species of the genus . It is concluded that the organism should be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Chem15 ( = IBRC-M 10490 = LMG 28544).

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2015-09-01
2020-01-27
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References

  1. Adachi K., Katsuta A., Matsuda S., Peng X., Misawa N., Shizuri Y., Kroppenstedt R.M., Yokota A., Kasai H.. ( 2007;). Smaragdicoccus niigatensis gen. nov., sp. nov., a novel member of the suborder Corynebacterineae. Int J Syst Evol Microbiol 57: 297–301 [CrossRef] [PubMed].
    [Google Scholar]
  2. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J.. ( 1997;). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402 [CrossRef] [PubMed].
    [Google Scholar]
  3. Bauer A.W., Kirby W.M.M., Sherris J.C., Turck M.. ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493–496 [PubMed].
    [Google Scholar]
  4. Camas M., Veyisoglu A., Sahin N.. ( 2014;). Nocardia sungurluensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 64: 1629–1634 [CrossRef] [PubMed].
    [Google Scholar]
  5. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221–230 [CrossRef] [PubMed].
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 [CrossRef] [PubMed].
    [Google Scholar]
  7. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  8. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R..), ( 1994;). Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  9. Goodfellow M., Maldonado L.A.. ( 2006;). The families Dietziaceae, Gordoniaceae, Nocardiaceae and Tsukamurellaceae. . In The Prokaryotes: a Handbook on the Biology of Bacteria, Archaea, Bacteria, Firmicutes, Actinomycetes3, 3rd edn., pp. 843–888. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E.. New York: [CrossRef] Springer;.
    [Google Scholar]
  10. Goodfellow M., Isik K., Yates E.. ( 1999;). Actinomycete systematics: an unfinished synthesis. Nova Acta Leopoldina NF80: 47–82.
    [Google Scholar]
  11. Gordon R.E.. ( 1967;). The taxonomy of soil bacteria. . In The Ecology of Soil Bacteria, pp. 293–321. Edited by Gray T. R. G., Parkinson D.. Liverpool: Liverpool University Press;.
    [Google Scholar]
  12. Gordon R.E., Barnett D.A., Handerhan J.E., Pang C.H.-N.. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24: 54–63 [CrossRef].
    [Google Scholar]
  13. Huss V.A.R., Festl H., Schleifer K.H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4: 184–192 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kelly K.L.. ( 1964;). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Print Office;.
    [Google Scholar]
  15. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  16. Küster E., Williams S.T.. ( 1964;). Selection of media for isolation of Streptomyces. Nature 202: 928–929 [CrossRef] [PubMed].
    [Google Scholar]
  17. Lechevalier M.P., Lechevalier H.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20: 435–443 [CrossRef].
    [Google Scholar]
  18. Li W.-J., Jiang Y., Kroppenstedt R.M., Xu L.H., Jiang C.L.. ( 2004;). Nocardia alba sp.nov., a novel actinomycete strain isolated from soil in China. Syst Appl Microbiol 27: 308–312 [CrossRef] [PubMed].
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  20. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H.. ( 1984;). An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  21. Nikou M.M., Ramezani M., Amoozegar M.A., Fazeli S.A.S., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A.. ( 2014;). Alloactinosynnema iranicum sp. nov., a rare actinomycete isolated from a hypersaline wetland, and emended description of the genus Alloactinosynnema. Int J Syst Evol Microbiol 64: 1173–1179 [CrossRef] [PubMed].
    [Google Scholar]
  22. Pospiech N.. ( 1995;). Practical streptomyces genetics. . pp. 169–170. Edited by Kieser T. J., Bibb M. J., Buttner M. F., Chater K. A., Hopwood D.. (2000);;. John Innes Foundation;.
  23. Rzhetsky A., Nei M.. ( 1992;). A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9: 945–967.
    [Google Scholar]
  24. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  25. Shirling E.B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313–340 [CrossRef].
    [Google Scholar]
  26. Staneck J.L., Roberts G.D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28: 226–231 [PubMed].
    [Google Scholar]
  27. Tamura K., Stecher G., Peterson D., Flipski A., Kumar S.. ( 2013;). mega6: Molecular evolutionary genetics analysis verson 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  28. Waksman S.A.. ( 1961;). The Actinomycetes. Classification, Identification and Descriptions of Genera and SpeciesVol. 2. Baltimore, MD: Williams Wilkins;.
    [Google Scholar]
  29. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  30. Williams S.T., Goodfellow M., Alderson G., Wellington E.M.H., Sneath P.H.A., Sackin M.J.. ( 1983;). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129: 1743–1813 [PubMed].
    [Google Scholar]
  31. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16: 176–178.
    [Google Scholar]
  32. Zhang Z., Schwartz S., Wagner L., Miller W.. ( 2000;). A greedy algorithm for aligning DNA sequences. J Comput Biol 7: 203–214 [CrossRef] [PubMed].
    [Google Scholar]
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