A novel Gram-negative, aerobic, motile, short rod-shaped bacterium, designated J64T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Cells grew at 4–37 °C, at pH 5.5–10.0 and with 0–10 % NaCl. The strain was found to be oxidase- and catalase-positive. Phylogenetic analyses showed that strain J64T belongs to the genus Pseudomonas, forming a monophyletic group with Pseudomonas pachastrellae, Pseudomonas pertucinogena and ‘Pseudomonas denitrificans’. The 16S rRNA gene sequence similarity between strain J64T and type strains of all Pseudomonas species with validly published names was below 96.6 %. Low levels of DNA–DNA relatedness were found with respect to type strains of P. pachastrellae and P. pertucinogena, supporting the classification of strain J64T within a novel species of the genus Pseudomonas. Strain J64T contained C18 : 1ω7c (37.2 %), C16 : 0 (20.4 %), summed feature 3 (17.4 %; comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c) and C12 : 0 (7.6 %) as major cellular fatty acids. On the basis of the phenotypic and phylogenetic data, strain J64T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas sabulinigri sp. nov. is proposed. The type strain is J64T (=KCTC 22137T =JCM 14963T).
Anzai, Y., Kim, H., Park, J. Y., Wakabayashi, H. & Oyaizu, H.(2000). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol50, 1563–1589.[CrossRef][Google Scholar]
Buck, J. D.(1982). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol44, 992–993.
[Google Scholar]
De Ley, J.(1992). The proteobacteria: ribosomal RNA cistron similarities and bacterial taxonomy. In The Prokaryotes, 2nd edn, pp. 2111–2140. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39, 224–229.[CrossRef][Google Scholar]
Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17, 368–376.[CrossRef][Google Scholar]
Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution39, 783–791.[CrossRef][Google Scholar]
Gonzalez, J. M. & Saiz-Jimenez, C.(2002). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol4, 770–773.[CrossRef][Google Scholar]
Kawai, Y. & Yabuuchi, E.(1975).Pseudomonas pertucinogena sp. nov., an organism previously misidentified as Bordetella pertussis. Int J Syst Bacteriol25, 317–323.[CrossRef][Google Scholar]
Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16, 111–120.[CrossRef][Google Scholar]
King, E. O., Ward, M. K. & Raney, D. E.(1954). Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med44, 301–307.
[Google Scholar]
Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform5, 150–163.[CrossRef][Google Scholar]
Migula, W.(1894). Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe1, 235–238 (in German).
[Google Scholar]
Romanenko, L. A., Uchino, M., Falsen, E., Frolova, G. M., Zhukova, N. V. & Mikhailov, V. V.(2005).Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol55, 919–924.[CrossRef][Google Scholar]
Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4, 406–425.
[Google Scholar]
Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
Swofford, D. L.(2003).paup: Phylogenetic analysis using parsimony, version 4. Sunderland, MA: Sinauer Associates.
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25, 4876–4882.[CrossRef][Google Scholar]