1887

Abstract

A novel Gram-negative, aerobic, motile, short rod-shaped bacterium, designated J64, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Cells grew at 4–37 °C, at pH 5.5–10.0 and with 0–10 % NaCl. The strain was found to be oxidase- and catalase-positive. Phylogenetic analyses showed that strain J64 belongs to the genus , forming a monophyletic group with , and ‘’. The 16S rRNA gene sequence similarity between strain J64 and type strains of all species with validly published names was below 96.6 %. Low levels of DNA–DNA relatedness were found with respect to type strains of and , supporting the classification of strain J64 within a novel species of the genus . Strain J64 contained C 7 (37.2 %), C (20.4 %), summed feature 3 (17.4 %; comprising iso-C 2-OH and/or C 7) and C (7.6 %) as major cellular fatty acids. On the basis of the phenotypic and phylogenetic data, strain J64 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J64 (=KCTC 22137 =JCM 14963).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65866-0
2009-01-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/1/38.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65866-0&mimeType=html&fmt=ahah

References

  1. Anzai, Y., Kim, H., Park, J. Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Buck, J. D. ( 1982; ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  3. De Ley, J. ( 1992; ). The proteobacteria: ribosomal RNA cistron similarities and bacterial taxonomy. In The Prokaryotes, 2nd edn, pp. 2111–2140. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Gonzalez, J. M. & Saiz-Jimenez, C. ( 2002; ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4, 770–773.[CrossRef]
    [Google Scholar]
  8. Kawai, Y. & Yabuuchi, E. ( 1975; ). Pseudomonas pertucinogena sp. nov., an organism previously misidentified as Bordetella pertussis. Int J Syst Bacteriol 25, 317–323.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. King, E. O., Ward, M. K. & Raney, D. E. ( 1954; ). Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44, 301–307.
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Migula, W. ( 1894; ). Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1, 235–238 (in German).
    [Google Scholar]
  13. Romanenko, L. A., Uchino, M., Falsen, E., Frolova, G. M., Zhukova, N. V. & Mikhailov, V. V. ( 2005; ). Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 55, 919–924.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Swofford, D. L. ( 2003; ). paup: Phylogenetic analysis using parsimony, version 4. Sunderland, MA: Sinauer Associates.
  17. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65866-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65866-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error