Four alkaligenous, moderately halotolerant strains, designated ge09, ge10, ge14 and ge15, were isolated from the internal tissue of ginseng root and their taxonomic positions were investigated by using a polyphasic approach. Cells of the four strains were Gram-positive-staining, non-motile, short rods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains ge09 and ge10 formed one cluster and strains ge14 and ge15 formed another separate cluster within the genus . 16S rRNA gene sequence similarities with type strains of other species were less than 97 %. Levels of DNA–DNA relatedness among the four strains showed that strains ge09 and ge10 and strains ge14 and ge15 belonged to two separate species; the mean level of DNA–DNA relatedness between ge10 and ge14 was only 28.7 %. Their phenotypic and physiological properties supported the view that the two strains represent two different novel species of the genus . The DNA G+C contents of strains ge10 and ge14 were 49.9 and 49.6 mol%, respectively. Strains ge10 and ge14 showed the peptidoglycan type A4 -Lys–-Glu. The lipids present in strains ge10 and ge14 were diphosphatidylglycerol, phosphatidylglycerol, a minor amount of phosphatidylcholine and two unknown phospholipids. Their predominant respiratory quinone was MK-7. The fatty acid profiles of the four novel strains contained large quantities of branched and saturated fatty acids. The predominant cellular fatty acids were iso-C (42.5 %), anteiso-C (22.2 %), anteiso-C (7.3 %) and C 7 alcohol (5.7 %) in ge10 and iso-C (50.7 %) and anteiso-C (20.1 %) in ge14. On the basis of their phenotypic properties and phylogenetic distinctiveness, two novel species of the genus are proposed, sp. nov. (type strain ge10 =DSM 19037 =CGMCC 1.6762) and sp. nov. (type strain ge14 =DSM 19038 =CGMCC 1.6763).


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vol. , part 4, pp. 729 - 734

Comparison of carbon source utilization among strains ge09, ge10 , ge14 and ge15.

Fatty acid profiles of strains ge09, ge10 , ge14 and ge15 measured by GC.

[PDF file of Supplementary Tables S1 and S2](118 KB)

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