During a study on lactic acid bacteria (and their species diversity) in spontaneous heap fermentations of Ghanaian cocoa beans, two strains, designated 215 and 194B, were isolated. A phylogenetic analysis based on 16S rRNA gene sequences demonstrated that these strains represented a distinct lineage close to the genus and showing only 92.1 % 16S rRNA gene sequence similarity with respect to their closest neighbour, LMG 20113. Whole-cell protein electrophoresis, fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and physiological and biochemical tests confirmed the unique taxonomic position of the two novel isolates. On the basis of the results of the morphological and biochemical tests and 16S rRNA gene sequence analysis, strains 215 and 194B represent the most peripheral lineage of the genus , for which we propose the name sp. nov. The type strain is 215 (=LMG 24286=DSM 19935).


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  1. Björkroth, K. J., Schillinger, U., Geisen, R., Weiss, N., Hoste, B., Holzapfel, W. H., Korkeala, H. J. & Vandamme, P.(2002). Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples. Int J Syst Evol Microbiol 52, 141–148. [Google Scholar]
  2. Camu, N., De Winter, T., Verbrugghe, K., Cleenwerck, I., Takrama, J. S., Vancanneyt, M. & De Vuyst, L.(2007). Dynamics and biodiversity of lactic acid bacteria and acetic acid bacteria populations involved in spontaneous heap fermentations of cocoa beans in Ghana. Appl Environ Microbiol 73, 1809–1824.[CrossRef] [Google Scholar]
  3. Chen, J. S., Witzmann, K. A., Spilker, T., Fink, R. J. & LiPuma, J. J.(2001). Endemicity and inter-city spread of Burkholderia cepacia genomovar III in cystic fibrosis. J Pediatr 139, 643–649.[CrossRef] [Google Scholar]
  4. Choi, H. J., Cheigh, C. I., Kim, S. B., Lee, J. C., Lee, D. W., Choi, S. W., Park, J. M. & Pyun, Y. R.(2002).Weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi. Int J Syst Evol Microbiol 52, 507–511. [Google Scholar]
  5. Coenye, T., Spilker, T., Martin, A. & LiPuma, J. J.(2002). Comparative assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar III. J Clin Microbiol 40, 3300–3307.[CrossRef] [Google Scholar]
  6. Collins, M. D., Samelis, J., Metaxopoulos, J. & Wallbanks, S.(1993). Taxonomic studies on some leuconostoc-like organisms from fermented sausages: description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. J Appl Bacteriol 75, 595–603.[CrossRef] [Google Scholar]
  7. Ennahar, S. & Cai, Y.(2004). Genetic evidence that Weissella kimchii Choi et al. 2002 is a later heterotypic synonym of Weissella cibaria Björkroth et al. 2002. Int J Syst Evol Microbiol 54, 463–465.[CrossRef] [Google Scholar]
  8. Franz, C. M. A. P., Vancanneyt, M., Vandemeulebroecke, K., De Wachter, M., Cleenwerck, I., Hoste, B., Schillinger, U., Holzapfel, W. H. & Swings, J.(2006).Pediococcus stilesii sp. nov., isolated from maize grains. Int J Syst Evol Microbiol 56, 329–333.[CrossRef] [Google Scholar]
  9. Lee, J. S., Lee, K. C., Ahn, J. S., Mheen, T. I., Pyun, Y. R. & Park, Y. H.(2002).Weissella koreensis sp. nov., isolated from kimchi. Int J Syst Evol Microbiol 52, 1257–1261.[CrossRef] [Google Scholar]
  10. Magnusson, J., Jonsson, H., Schnurer, J. & Roos, S.(2002).Weissella soli sp. nov., a lactic acid bacterium isolated from soil. Int J Syst Evol Microbiol 52, 831–834.[CrossRef] [Google Scholar]
  11. Mahenthiralingam, E., Campbell, M. E., Henry, D. A. & Speert, D. P.(1996). Epidemiology of Burkholderia cepacia infection in patients with cystic fibrosis: analysis by randomly amplified polymorphic DNA fingerprinting. J Clin Microbiol 34, 2914–2920. [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  13. Pot, B., Vandamme, P. & Kersters, K.(1994). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester, UK: Wiley.
  14. Tanasupawat, S., Shida, O., Okada, S. & Komagata, K.(2000).Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50, 1479–1485.[CrossRef] [Google Scholar]
  15. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  16. Vancanneyt, M., Mengaud, J., Cleenwerck, I., Vanhonacker, K., Hoste, B., Dawyndt, P., Degivry, M. C., Ringuet, D., Janssens, D. & Swings, J.(2004). Reclassification of Lactobacillus kefirgranum Takizawa et al. 1994 as Lactobacillus kefiranofaciens subsp. kefirgranum subsp. nov. and emended description of L. kefiranofaciens Fujisawa et al. 1988. Int J Syst Evol Microbiol 54, 551–556.[CrossRef] [Google Scholar]

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Neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, for strain LMG 24286 together with recognized strains and their phylogenetically closest neighbours. [ PDF] 48 KB


SDS-PAGE protein profiles and corresponding cluster analysis derived from UPGMA linkage of correlation coefficients for strain 215 , strain 194B and the type strains of all established species. [ PDF] 63 KB

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