1887

Abstract

During a study on lactic acid bacteria (and their species diversity) in spontaneous heap fermentations of Ghanaian cocoa beans, two strains, designated 215 and 194B, were isolated. A phylogenetic analysis based on 16S rRNA gene sequences demonstrated that these strains represented a distinct lineage close to the genus and showing only 92.1 % 16S rRNA gene sequence similarity with respect to their closest neighbour, LMG 20113. Whole-cell protein electrophoresis, fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and physiological and biochemical tests confirmed the unique taxonomic position of the two novel isolates. On the basis of the results of the morphological and biochemical tests and 16S rRNA gene sequence analysis, strains 215 and 194B represent the most peripheral lineage of the genus , for which we propose the name sp. nov. The type strain is 215 (=LMG 24286=DSM 19935).

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2008-12-01
2019-10-20
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Neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, for strain LMG 24286 together with recognized strains and their phylogenetically closest neighbours. [ PDF] 48 KB

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SDS-PAGE protein profiles and corresponding cluster analysis derived from UPGMA linkage of correlation coefficients for strain 215 , strain 194B and the type strains of all established species. [ PDF] 63 KB

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