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Three strains of xylanase-producing bacteria, S5-3T, X13-1T and MXC2-2T, isolated from soils in Thailand, were characterized taxonomically based on their phenotypic and chemotaxonomic characteristics and 16S rRNA gene sequence comparisons. They were Gram-positive, facultatively anaerobic, spore-forming, rod-shaped bacteria. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The DNA G+C contents were respectively 45.8, 47.3 and 48.8 mol%. The predominant isoprenoid quinone was MK-7, and anteiso-C15 : 0 and C16 : 0 were the dominant cellular fatty acids. Phylogenetic analyses based on 16S rRNA gene sequence comparison showed that they were affiliated to the genus Paenibacillus. Strains S5-3T, X13-1T and MXC2-2T were closely related to Paenibacillus granivorans A30T and Paenibacillus agaridevorans DSM 1355T (94.7–98.6 % 16S rRNA gene sequence similarity). The similarity between the three novel strains ranged from 96.3 to 98.4 %. DNA–DNA relatedness, DNA G+C contents and some phenotypic characteristics could clearly distinguish the strains from each other and from related Paenibacillus species. Therefore, strains S5-3T, X13-1T and MXC2-2T represent novel species of the genus Paenibacillus, for which the names Paenibacillus siamensis sp. nov. (type strain S5-3T =KCTC 13038T =PCU 279T =TISTR 1831T), Paenibacillus septentrionalis sp. nov. (type strain X13-1T =KCTC 13039T =PCU 280T =TISTR 1830T) and Paenibacillus montaniterrae sp. nov. (type strain MXC2-2T =KCTC 13036T =PCU 281T =TISTR 1836T) are proposed.
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International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 1, pp. 130 - 134
Supplementary Figs S1 and S2. Neighbour-joining (S1) and maximum-parsimony (S2) trees of 16S rRNA gene sequences showing the phylogenetic relationships between strains S5-3 T, X13-1 Tand MXC2-2 Tand all Paenibacillus species. [PDF](61 KB)