Three strains of xylanase-producing bacteria, S5-3, X13-1 and MXC2-2, isolated from soils in Thailand, were characterized taxonomically based on their phenotypic and chemotaxonomic characteristics and 16S rRNA gene sequence comparisons. They were Gram-positive, facultatively anaerobic, spore-forming, rod-shaped bacteria. They contained -diaminopimelic acid in the cell-wall peptidoglycan. The DNA G+C contents were respectively 45.8, 47.3 and 48.8 mol%. The predominant isoprenoid quinone was MK-7, and anteiso-C and C were the dominant cellular fatty acids. Phylogenetic analyses based on 16S rRNA gene sequence comparison showed that they were affiliated to the genus . Strains S5-3, X13-1 and MXC2-2 were closely related to A30 and DSM 1355 (94.7–98.6 % 16S rRNA gene sequence similarity). The similarity between the three novel strains ranged from 96.3 to 98.4 %. DNA–DNA relatedness, DNA G+C contents and some phenotypic characteristics could clearly distinguish the strains from each other and from related species. Therefore, strains S5-3, X13-1 and MXC2-2 represent novel species of the genus , for which the names sp. nov. (type strain S5-3 =KCTC 13038 =PCU 279 =TISTR 1831), sp. nov. (type strain X13-1 =KCTC 13039 =PCU 280 =TISTR 1830) and sp. nov. (type strain MXC2-2 =KCTC 13036 =PCU 281 =TISTR 1836) are proposed.


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vol. , part 1, pp. 130 - 134

Neighbour-joining (S1) and maximum-parsimony (S2) trees of 16S rRNA gene sequences showing the phylogenetic relationships between strains S5-3 , X13-1 and MXC2-2 and all species. [PDF](61 KB)

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