A Gram-negative, non-motile, neutrophilic, rod-shaped, extremely halophilic archaeon, designated strain BG-1, was isolated from a salt lake, Lake Bagaejinnor, in Inner Mongolia, China. Strain BG-1 was able to grow at 25–55 °C, required at least 2.5 M NaCl for growth (with an optimum at 3.4 M NaCl) and grew at pH 6.0–9.0 (with an optimum at pH 7.5). Hypotonic treatment with less than 2.0 M NaCl caused cell lysis. Phylogenetic analysis of the almost-complete 16S rRNA gene sequence positioned the isolate within the genus in the family . Strain BG-1 was most closely related to 31-hong (98.8 % sequence similarity), NCIMB 2081 (98.6 %), ACAM 34 (98.6 %) and 9-3 (98.4 %). However, values for DNA–DNA hybridization between strain BG-1 and the most closely related members of the genus were below 40 %. Analysis of the polar lipids of strain BG-1 revealed the presence of mannosyl-2-sulfate-(1-4)-glycosyl-archaeol, the main glycolipid found in neutrophilic species of the genus . The G+C content of the genomic DNA was 69.4 mol% ( ). Comparison of the phenotypic characteristics of the strain with those of species supported the conclusion that BG-1 represents a novel species within this genus, for which the name sp. nov. is proposed. The type strain is BG-1 (=CECT 7322 =CGMCC 1.7018 =JCM 14978).


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vol. , part 9, pp. 2031 - 2035

Thin-layer chromatogram of polar lipids extracted from strain BG-1 and some haloarchaea. [ PDF] 34 KB


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