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A Gram-positive, psychrophilic, rod-shaped bacterium, designated strain N-05T, was isolated from soil samples collected off King George Island, west Antarctica (6 ° 13′ 31″ S 5 ° 57′ 08″ W). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N-05T was related to members of the genus Sporosarcina and had highest 16S rRNA gene sequence similarity with the type strain of Sporosarcina macmurdoensis (98.0 %). The temperature range for growth of strain N-05T was 0–23 °C, with optimum growth occurring at 17–18 °C and approximately pH 6.0–8.0. Strain N-05T had MK-7 as the major menaquinone and anteiso-C15 : 0 and C16 : 1 ω7c alcohol as major fatty acids. The genomic DNA G+C content was 39.2 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain N-05T is considered to represent a novel species of the genus Sporosarcina, for which the name Sporosarcina antarctica is proposed. The type strain is N-05T (=CGMCC 1.6503T=JCM 14646T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 9, pp. 2114 - 2117
Supplementary Table S1. Comparison of cellular fatty acid contents of strain N-05 T( Sporosarcina antarctica sp. nov.) and other recognized Sporosarcina species. [PDF](47 KB)