1887

Abstract

A Gram-positive, small coccus-shaped lactic acid bacterium, strain LMG 23999, was isolated from Argentinean wheat flour. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus , with and as its closest relatives (97.7, 97.3 and 96.9 % gene sequence similarity, respectively). Fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and whole-cell protein electrophoresis confirmed the unique taxonomic status of the novel strain. DNA–DNA hybridizations, DNA G+C content determination, comparative sequence analysis of the , and genes and physiological and biochemical characterization demonstrated that strain LMG 23999 (=CCUG 54535=CRL 776) represents a novel species for which the name sp. nov. is proposed. Multi-locus sequence analysis based on , and genes was found to be a suitable method for the identification of species of the genus .

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2008-12-01
2019-10-13
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References

  1. Collins, M. D., Williams, A. M. & Wallbanks, S. ( 1990; ). The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov. FEMS Microbiol Lett 58, 255–262.
    [Google Scholar]
  2. Collins, M. D., Rodrigues, U., Ash, C., Aguirre, M., Farrow, J. A. E., Martinez-Murcia, A., Phillips, B. A., Williams, A. M. & Wallbanks, S. ( 1991; ). Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA. FEMS Microbiol Lett 77, 5–12.[CrossRef]
    [Google Scholar]
  3. De Bruyne, K., Schillinger, U., Caroline, L., Böhringer, B., Cleenwerck, I., Vancanneyt, M., De Vuyst, L., Franz, C. M. A. P. & Vandamme, P. ( 2007; ). Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species. Int J Syst Evol Microbiol 57, 2952–2959.[CrossRef]
    [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  5. Dobson, C. M., Deneer, H., Lee, S., Hemmingsen, S., Glaze, S. & Ziola, B. ( 2002; ). Phylogenetic analysis of the genus Pediococcus, including Pediococcus claussenii sp. nov., a novel lactic acid bacterium isolated from beer. Int J Syst Evol Microbiol 52, 2003–2010.[CrossRef]
    [Google Scholar]
  6. Franz, C. M. A. P., Vancanneyt, M., Vandemeulebroecke, K., De Wachter, M., Cleenwerck, I., Hoste, B., Schillinger, U., Holzapfel, W. H. & Swings, J. ( 2006; ). Pediococcus stilesii sp. nov., isolated from maize grains. Int J Syst Evol Microbiol 56, 329–333.[CrossRef]
    [Google Scholar]
  7. Garvie, E. I. ( 1974; ). Nomenclatural problems of the pediococci. Request for an Opinion. Int J Syst Bacteriol 24, 301–306.[CrossRef]
    [Google Scholar]
  8. Garvie, E. I. ( 1986; ). Genus Pediococcus Claussen 1903, 68AL. In Bergey's Manual of Systematic Bacteriology, pp. 1075–1079. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  9. Holzapfel, W. H., Franz, C. M. A. P., Ludwig, W., Back, W. & Dicks, L. M. T. ( 2005; ). Genera Pediococcus and Tetragenococcus. In The Prokaryotes, 3rd edn. An Evolving Electronic Resource for the Microbiological Community, release 3.15. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer-Verlag.
  10. Konstantinidis, K. T., Ramette, A. & Tiedje, J. M. ( 2006; ). Towards a more robust assessment of intra-species diversity using fewer genetic markers. Appl Environ Microbiol 72, 7286–7293.[CrossRef]
    [Google Scholar]
  11. Leroy, F. & De Vuyst, L. ( 2004; ). Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci Technol 15, 67–78.[CrossRef]
    [Google Scholar]
  12. Liu, L., Zhang, B., Tong, H. & Dong, X. ( 2006; ). Pediococcus ethanolidurans sp. nov., isolated from the walls of a distilled-spirit-fermenting cellar. Int J Syst Evol Microbiol 56, 2405–2408.[CrossRef]
    [Google Scholar]
  13. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Naser, S., Thompson, F. L., Hoste, B., Gevers, D., Vandemeulebroecke, K., Cleenwerck, I., Thompson, C. C., Vancanneyt, M. & Swings, J. ( 2005a; ). Phylogeny and identification of Enterococci by atpA gene sequence analysis. J Clin Microbiol 43, 2224–2230.[CrossRef]
    [Google Scholar]
  16. Naser, S. M., Thompson, F. L., Hoste, B., Gevers, D., Dawyndt, P., Vancanneyt, M. & Swings, J. ( 2005b; ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151, 2141–2150.[CrossRef]
    [Google Scholar]
  17. Naser, S. M., Dawyndt, P. S. R., Hoste, B., Gevers, D., Vandemeulebroecke, K., Cleenwerck, I., Vancanneyt, M. & Swings, J. ( 2007; ). Identification of lactobacilli by pheS and rpoA gene sequence analyses. Int J Syst Evol Microbiol 57, 2777–2789.[CrossRef]
    [Google Scholar]
  18. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester, UK: Wiley.
  19. Renouf, V., Claisse, O. & Lonvaud-Funel, A. ( 2007; ). Inventory and monitoring of wine microbial consortia. Appl Microbiol Biotechnol 75, 149–164.[CrossRef]
    [Google Scholar]
  20. Sakamoto, K. & Konings, W. N. ( 2003; ). Beer spoilage bacteria and hop resistance. Int J Food Microbiol 89, 105–124.[CrossRef]
    [Google Scholar]
  21. Schillinger, U. & Lücke, F.-K. ( 1987; ). Identification of lactobacilli from meat and meat products. Food Microbiol 4, 199–208.[CrossRef]
    [Google Scholar]
  22. Simpson, W. J. & Taguchi, H. ( 1995; ). The genus Pediococcus, with notes on the genera Tetragenococcus and Aerococcus. In The Genera of Lactic Acid Bacteria, pp. 125–172. Edited by B. J. B. Wood & W. H. Holzapfel. Blackie Academic & Professional.
  23. Sneath, P. H. A., Mair, N. S., Sharpe, M. E. & Holt, J. G. ( 1986; ). Bergey's Manual of Systematic Bacteriology. Edited by J. P. Butler. Baltimore, USA: Williams & Wilkins.
  24. Stackebrandt, E. & Kandler, O. ( 1979; ). Taxonomy of the genus Cellulomonas, based on phenotypic characters and deoxyribonucleic acid-deoxyribonucleic acid homology and proposal of seven neotype strains. Int J Syst Bacteriol 29, 273–282.[CrossRef]
    [Google Scholar]
  25. Stiles, M. E. & Holzapfel, W. H. ( 1997; ). Lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol 36, 1–29.[CrossRef]
    [Google Scholar]
  26. Tanasupawat, S., Pakdeeto, A., Thawai, C., Yukphan, P. & Okada, S. ( 2007; ). Identification of lactic acid bacteria from fermented tea leaves (miang) in Thailand and proposals of Lactobacillus thailandensis sp. nov., Lactobacillus camelliae sp. nov., and Pediococcus siamensis sp. nov. J Gen Appl Microbiol 53, 7–15.[CrossRef]
    [Google Scholar]
  27. Vancanneyt, M., Mengaud, J., Cleenwerck, I., Hoste, B., Dawyndt, P., Degivry, M. C., Ringuet, D., Janssens, D. & Swings, J. ( 2004; ). Reclassification of Lactobacillus kefirgranum Takizawa et al. 1994 as Lactobacillus kefiranofaciens subsp. kefirgranum subsp. nov. and emended description of L. kefiranofaciens Fujisawa et al. 1988. Int J Syst Evol Microbiol 54, 551–556.[CrossRef]
    [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  29. Zhang, B., Tong, H. & Dong, X. ( 2005; ). Pediococcus cellicola sp. nov., a novel lactic acid coccus isolated from a distilled-spirit-fermenting cellar. Int J Syst Evol Microbiol 55, 2167–2170.[CrossRef]
    [Google Scholar]
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Genomes used for the design of primers rpoA-21-F* and rpoA-23-R*. [ PDF] 41 KB

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Cluster analysis of whole-cell protein profiles and dendrogram derived from UPGMA linkage of correlation coefficients of sp. nov. LMG 23999 and representatives of its closest phylogenetic neighbours. [ PDF] 45 KB

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Phylogenetic trees based on neighbour-joining method using (a), (b) and (c) gene sequences of strains and type strains of all species belonging to the group. [ PDF] 66 KB

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