A coryneform bacterium isolated from a cow with a profound ulceration was characterized by using phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and short-chain mycolic acids consistent with the genus . Comparative 16S rRNA gene sequencing showed that the organism formed a hitherto unknown subline within the genus . Sequence divergence values of greater than 3.6 % from recognized species, together with phenotypic differences, showed that the unidentified bacterium represents a new member of this genus. On the basis of phenotypic and phylogenetic considerations, it is proposed that the novel bacterium be classified as representing a novel species of the genus , with the name sp. nov. The type strain is IMMIB L-1395 (=DSM 45146=CCUG 55727).


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vol. , part 1, pp. 34 - 37

Extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequences. [PDF](24 KB)


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