During a survey of the mycobiota in the stone chamber of the Takamatsu-zuka tumulus in the village of Asuka, Nara Prefecture, Japan, we isolated 19 yeast strains assigned to the genus from various samples, taken mainly from mouldy spots where the colour of the murals had changed to black, white or another tone, and from viscous gels (biofilms) on plaster walls. The 26S rDNA D1/D2 domain sequence-based phylogeny clearly indicates two groups of isolates. Polyphasic characterization, including morphological, physiological and chemotaxonomic characteristics, and sequence analysis of the 26S rDNA D1/D2 domain and ITS regions suggest that each group is assignable to one of two novel species within the clade. Proposed herein are the names sp. nov. (originally T6517-9-5; holotype JCM 15403; isotypes CBS 10917, NBRC 104392) and sp. nov. (originally T4922-1-1; holotype JCM 15410; isotypes CBS 10916, NBRC 104391). The 26S rDNA D1/D2 domain sequence divergence indicates that differs from NBRC 10277, the type strain of the nearest species, in 15 nucleotides (3 %), whereas differs from NBRC 10283 and NBRC 10544, the type strains of the nearest species, in 20 nucleotides (4 %). Both novel species are also clearly distinguishable from the species closest to them by various physiological characteristics.


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vol. , part 1, pp. 186 - 194

Phylogenetic position of sp. nov. T6517-9-5 (group A) and sp. nov. T4922-1-1 (group B) in the clade, excluding subclade C, following maximum-parsimony analysis of 26S rDNA D1/D2 domain sequences (497 nt). [PDF](30 KB)


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