1887

Abstract

Five rhizobial strains representing the largest group in the genus associated with spp. in China were characterized taxonomically. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these microsymbionts belonged to the genus , with USDA 3592, SDW018 and UPM-Ca36 as the closest neighbours (≥99.5 % 16S rRNA gene sequence similarity). Genotypic fingerprinting by whole-cell protein electrophoresis, DNA–DNA hybridization, comparative housekeeping sequence analysis of the , and genes, fatty acid profiles and a series of phenotypic and physiological tests allowed the novel group to be differentiated from all previously recognized species of the genus . This group therefore represents a novel species, for which the name sp. nov. is proposed with the type strain CCBAU 11299 (=LMG 24397=HAMBI 2990). Cross-inoculation tests showed that strain CCBAU 11299 could form effective nodules on , , , and .

Keyword(s): NJ, neighbour-joining
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65829-0
2008-11-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/11/2646.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65829-0&mimeType=html&fmt=ahah

References

  1. Chen, W. X., Li, G. S., Qi, Y. L., Wang, E. T., Yuan, H. L. & Li, J. L. ( 1991; ). Rhizobium huakuii sp. nov., isolated from the root nodules of Astragalus sinicus. Int J Syst Bacteriol 41, 275–280.[CrossRef]
    [Google Scholar]
  2. Chen, W. X., Wang, E. T., Wang, S. Y., Li, Y. B., Chen, X. Q. & Li, Y. ( 1995; ). Characteristics of Rhizobium tianshanense sp. nov., a moderately and slowly growing root nodule bacterium isolated from an arid saline environment in Xinjiang, People's Republic of China. Int J Syst Bacteriol 45, 153–159.[CrossRef]
    [Google Scholar]
  3. de Lajudie, P., Willems, A., Nick, G., Moreira, F., Molouba, F., Hoste, B., Torck, U., Neyra, M., Collins, M. D. & other authors ( 1998; ). Characterization of tropical tree rhizobia and description of Mesorhizobium plurifarium sp. nov. Int J Syst Bacteriol 48, 369–382.[CrossRef]
    [Google Scholar]
  4. De Ley, J. ( 1970; ). Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101, 738–754.
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Gao, J. L., Sun, J. G., Li, Y., Wang, E. T. & Chen, W. X. ( 1994; ). Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan Province, China. Int J Syst Bacteriol 44, 151–158.[CrossRef]
    [Google Scholar]
  7. Gao, J. L., Terefework, Z., Chen, W. X. & Lindström, K. ( 2001; ). Genetic diversity of rhizobia isolated from Astragalus adsurgens growing in different geographical regions of China. J Biotechnol 91, 155–168.[CrossRef]
    [Google Scholar]
  8. Gao, J. L., Turner, S. L., Kan, F. L., Wang, E. T., Tan, Z. Y., Qiu, Y. H., Gu, J., Terefework, Z., Young, J. P. & other authors ( 2004; ). Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov. isolated from Astragalus adsurgens growing in the northern regions of China. Int J Syst Evol Microbiol 54, 2003–2012.[CrossRef]
    [Google Scholar]
  9. Gaunt, M. W., Turner, S. L., Rigottier-Gois, L., Lioyd-Macgilp, S. A. & Young, J. P. W. ( 2001; ). Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. Int J Syst Evol Microbiol 51, 2037–2048.[CrossRef]
    [Google Scholar]
  10. Ghosh, W. & Roy, P. ( 2006; ). Mesorhizobium thiogangeticum sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an Indian tropical leguminous plant. Int J Syst Evol Microbiol 56, 91–97.[CrossRef]
    [Google Scholar]
  11. Jarvis, B. D. W., van Berkum, P., Chen, W. X., Nour, S. M., Fernandez, M. P., Cleyet-Marel, J. C. & Gillis, M. ( 1997; ). Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 47, 895–898.[CrossRef]
    [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  13. Laguerre, G., Nour, S. M., Macheret, V., Sanjuan, J., Drouin, P. & Amarger, N. ( 2001; ). Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology 147, 981–993.
    [Google Scholar]
  14. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  15. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  16. Martens, M., Dawyndt, P., Coopman, R., Gillis, M., De Vos, P. & Willems, A. ( 2008; ). Advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus Ensifer (including former Sinorhizobium). Int J Syst Evol Microbiol 58, 200–214.[CrossRef]
    [Google Scholar]
  17. Nour, S. M., Fernandez, M. P., Normand, P. & Cleyet-Marel, J.-C. ( 1994; ). Rhizobium ciceri sp. nov., consisting of strains that nodulate chickpeas (Cicer arietinum L.). Int J Syst Bacteriol 44, 511–522.[CrossRef]
    [Google Scholar]
  18. Nour, S. M., Cleyet-Marel, J.-C., Normand, P. & Fernandez, M. P. ( 1995; ). Genomic heterogeneity of strains nodulating chickpeas (Cicer arietinum L.) and description of Rhizobium mediterraneum sp. nov. Int J Syst Bacteriol 45, 640–648.[CrossRef]
    [Google Scholar]
  19. Parker, M. A. ( 2004; ). rRNA and dnaK relationships of Bradyrhizobium sp. nodule bacteria from four papilionoid legume trees in Costa Rica. Syst Appl Microbiol 27, 334–342.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  22. Schutter, M. E. & Dick, R. P. ( 2000; ). Comparison of fatty acid methyl ester (FAME) methods for characterizing microbial communities. Soil Sci Soc Am J 64, 1659–1668.[CrossRef]
    [Google Scholar]
  23. Stepkowski, T., Czaplinska, M., Miedzinska, K. & Moulin, L. ( 2003; ). The variable part of the dnaK gene as an alternative marker for phylogenetic studies of rhizobia and related alpha Proteobacteria. Syst Appl Microbiol 26, 483–494.[CrossRef]
    [Google Scholar]
  24. Su, Y. Z., Zhang, T. H., Li, Y. L. & Wang, F. ( 2005; ). Changes in soil properties after establishment of Artemisia halodendron and Caragana microphylla on shifting sand dunes in semiarid Horqin Sandy Land, northern China. Environ Manage 36, 272–281.[CrossRef]
    [Google Scholar]
  25. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  26. Tan, Z. Y., Wang, E. T., Gao, J. L., Martínez-Romero, E. & Chen, W. X. ( 1997; ). Phylogenetic and genetic relationships of Mesorhizobium tianshanense and related rhizobia. Int J Syst Bacteriol 47, 874–879.[CrossRef]
    [Google Scholar]
  27. Tighe, S. W., de Lajudie, P., Dipietro, K., Lindström, K., Nick, G. & Jarvis, B. D. W. ( 2000; ). Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol 50, 787–801.[CrossRef]
    [Google Scholar]
  28. Turner, S. L. & Young, J. P. W. ( 2000; ). The glutamine synthetases of rhizobia: phylogenetics and evolutionary implications. Mol Biol Evol 17, 309–319.[CrossRef]
    [Google Scholar]
  29. Vauterin, L. & Vauterin, P. ( 1992; ). Computer-aided objective comparison of electrophoresis patterns for grouping and identification of microorganisms. Eur Microbiol 1, 37–41.
    [Google Scholar]
  30. Velázquez, E., Igual, J. M., Willems, A., Fernández, M. P., Muñoz, E., Mateos, P. F., Abril, A., Toro, N., Normand, P. & other authors ( 2001; ). Mesorhizobium chacoense sp. nov., a novel species that nodulates Prosopis alba in the Chaco Arido region (Argentina). Int J Syst Evol Microbiol 51, 1011–1021.[CrossRef]
    [Google Scholar]
  31. Vincent, J. M. ( 1970; ). The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of the Root-Nodule Bacteria, pp. 1–13. Edited by J. M. Vincent. Oxford: Blackwell Scientific.
  32. Vinuesa, P., León-Barrios, M., Silva, C., Willems, A., Jarabo-Lorenzo, A., Pérez-Galdona, R., Werner, D. & Martínez-Romero, E. ( 2005; ). Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies α and Bradyrhizobium genospecies β. Int J Syst Evol Microbiol 55, 569–575.[CrossRef]
    [Google Scholar]
  33. Wang, E. T., van Berkum, P., Sui, X. H., Beyene, D., Chen, W. X. & Martínez-Romero, E. ( 1999; ). Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov. Int J Syst Bacteriol 49, 51–65.[CrossRef]
    [Google Scholar]
  34. Wang, F. Q., Wang, E. T., Liu, J., Chen, Q., Sui, X. H., Chen, W. F. & Chen, W. X. ( 2007; ). Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China. Int J Syst Evol Microbiol 57, 1192–1199.[CrossRef]
    [Google Scholar]
  35. Wernegreen, J. J. & Riley, M. A. ( 1999; ). Comparison of the evolutionary dynamics of symbiotic and housekeeping loci: a case for the genetic coherence of rhizobial lineages. Mol Biol Evol 16, 98–113.[CrossRef]
    [Google Scholar]
  36. Xiang, T., Uno, T., Ogino, F., Ai, C., Duo, J. & Sankawa, U. ( 2005; ). Antioxidant constituents of Caragana tibetica. Chem Pharm Bull (Tokyo) 53, 1204–1206.[CrossRef]
    [Google Scholar]
  37. Yan, X. R., Chen, W. F., Fu, J. F., Lu, Y. L., Xue, C. Y., Sui, X. H., Li, Y., Wang, E. T. & Chen, W. X. ( 2007; ). Mesorhizobium spp. are the main microsymbionts of Caragana spp. grown in Liaoning Province of China. FEMS Microbiol Lett 271, 265–273.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65829-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65829-0
Loading

Data & Media loading...

Supplements

Combined file [ PDF] 263 KB

PDF

Combined file [ PDF] 134 KB

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error