1887

Abstract

An aerobic, Gram-negative, non-pigmented, non-motile bacterium, KMM 3858, was isolated from a sea-ice sample collected from Peter the Great Bay of the Sea of Japan, Russia, and subjected to a phenotypic and phylogenetic study. Comparative analyses based on the 16S rRNA and gene sequences placed strain KMM 3858 within the genus . The major chemotaxonomic characteristics were found to be the presence of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminolipid and phosphatidylcholine, major fatty acids C 7 and C cyclo, and ubiquinone Q-10, confirming the affiliation of strain KMM 3858 to the genus . On the basis of the phylogenetic analysis and the physiological and biochemical characterization, strain KMM 3858 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain Pi26 (=KMM 3858=NRIC 0733=JCM 15115).

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2008-10-01
2019-10-16
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References

  1. Berg, G., Eberi, L. & Hartmann, A. ( 2005; ). The rhizosphere as a reservoir for opportunistic human pathogenic bacteria. Environ Microbiol 7, 1673–1685.[CrossRef]
    [Google Scholar]
  2. Bligh, E. G. & Dyer, W. J. ( 1959; ). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37, 911–917.[CrossRef]
    [Google Scholar]
  3. Denner, E. B. M., Paukner, S., Kämpfer, P., Moore, E. R. B., Abraham, W. R., Busse, H.-J., Wanner, G. & Lubitz, W. ( 2001; ). Sphingomonas pituitosa sp. nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan. Int J Syst Evol Microbiol 51, 827–841.[CrossRef]
    [Google Scholar]
  4. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  5. Holmes, B., Popoff, M., Kiredjian, M. & Kersters, K. ( 1988; ). Ochrobactrum anthropi gen. nov., sp. nov. from human clinical specimens and previously known as group Vd. Int J Syst Bacteriol 38, 406–416.[CrossRef]
    [Google Scholar]
  6. Hugh, R. & Leifson, E. ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66, 24–26.
    [Google Scholar]
  7. Kämpfer, P., Buczolits, S., Albrecht, A., Busse, H.-J. & Stackebrandt, E. ( 2003; ). Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov. Int J Syst Evol Microbiol 53, 893–896.[CrossRef]
    [Google Scholar]
  8. Kämpfer, P., Rosselló-Mora, R., Scholz, H. C., Welinder-Olsson, C., Falsen, E. & Busse, H.-J. ( 2006; ). Description of Pseudochrobactrum gen. nov., with the two species Pseudochrobactrum asaccharolyticum sp. nov. and Pseudochrobactrum saccharolyticum sp. nov. Int J Syst Evol Microbiol 56, 1823–1829.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P., Scholz, H. C., Huber, B., Falsen, E. & Busse, H.-J. ( 2007a; ). Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 57, 2513–2518.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Scholz, H., Huber, B., Thummes, K., Busse, H.-J., Maas, E. W. & Falsen, E. ( 2007b; ). Description of Pseudochrobactrum kiredjianiae sp. nov. Int J Syst Evol Microbiol 57, 755–760.[CrossRef]
    [Google Scholar]
  11. Lebuhn, M., Achouak, W., Schloter, M., Berge, O., Meier, H., Barakat, M., Hartmann, A. & Heulin, T. ( 2000; ). Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov. Int J Syst Evol Microbiol 50, 2207–2223.[CrossRef]
    [Google Scholar]
  12. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef]
    [Google Scholar]
  13. Romanenko, L. A., Uchino, M., Mikhailov, V. V., Zhukova, N. V. & Uchimura, T. ( 2003; ). Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. Int J Syst Evol Microbiol 53, 829–832.[CrossRef]
    [Google Scholar]
  14. Romanenko, L. A., Uchino, M., Falsen, E., Frolova, G. M., Zhukova, N. V. & Mikhailov, V. V. ( 2005; ). Pseudomonas pachastrellae sp. nov. isolated from a marine sponge. Int J Syst Evol Microbiol 55, 919–924.[CrossRef]
    [Google Scholar]
  15. Scholz, H. C., Tomaso, H., Al Dahouk, S., Witte, A., Schloter, M., Kämpfer, P., Falsen, E. & Neubauer, H. ( 2006; ). Genotyping of Ochrobactrum anthropi by recA-based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis. FEMS Microbiol Lett 257, 7–16.[CrossRef]
    [Google Scholar]
  16. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. ( 1997; ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef]
    [Google Scholar]
  17. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  18. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  21. Teyssier, C., Marchandin, H., Jean-Pierre, H., Masnou, A., Dusart, G. & Jumas-Bilak, E. ( 2007; ). Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples. Int J Syst Evol Microbiol 57, 1007–1013.[CrossRef]
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Tripathi, A. K., Verma, S. C., Chowdhury, S. P., Lebuhn, M., Gattinger, A. & Schloter, M. ( 2006; ). Ochrobactrum oryzae sp. nov., an endophytic bacterial species isolated from deep-water rice in India. Int J Syst Evol Microbiol 56, 1677–1680.[CrossRef]
    [Google Scholar]
  24. Trujillo, M. E., Willems, A., Abril, A., Planchuelo, A. M., Rivas, R., Ludeña, D., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2005; ). Nodulation of Lupinus albus by strains of Ochrobactrum lupini sp. nov. Appl Environ Microbiol 71, 1318–1327.[CrossRef]
    [Google Scholar]
  25. Velasco, J., Romero, C., López-Goñi, I., Leiva, J., Díaz, R. & Moriyón, I. ( 1998; ). Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp. Int J Syst Bacteriol 48, 759–768.[CrossRef]
    [Google Scholar]
  26. Zurdo-Piñeiro, J. L., Rivas, R., Trujillo, M. E., Vizcaíno, N., Carrasco, J. A., Chamber, M., Palomares, A., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2007; ). Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain. Int J Syst Evol Microbiol 57, 784–788.[CrossRef]
    [Google Scholar]
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Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences. [PDF](17 KB)

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Maximum-parsimony phylogenetic tree based on gene sequences. [PDF](13 KB)

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Two-dimensional thin-layer chromatogram of polar lipids of strain KMM 3858 ( sp. nov.). [PDF](36 KB)

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Fatty acid compositions of strain KMM 3858 ( sp. nov.) and the type strains of recognized and species. [PDF](76 KB)

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