1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 4

sp. nov., isolated from Mongolian jirds () Free

Abstract

Gram-positive, catalase-negative, chain-forming, coccus-shaped organisms were isolated both from intraperitoneally grown vesicles of the fox tapeworm and the oropharynges of laboratory-kept Mongolian jirds (). The strains displayed no haemolytic activity on Columbia sheep blood agar, pyrrolidonyl arylamidase activity was negative and the organisms reacted weakly with Lancefield group D antiserum. On the basis of phenotypic characteristics, the strains were tentatively identified as members of the genus . Comparative 16S rRNA gene sequencing studies confirmed their assignment to the genus and revealed that DSM 20770 was their closest phylogenetic neighbour (96.5 % sequence similarity). The levels of 16S rRNA gene sequence similarity between the isolates and representatives of species of the genus were only 95.7–96.2 %. On the basis of the phenotypic and molecular data presented, the isolates from Mongolian jirds represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WUE3771 (=DSM 19192=CCUG 54871).

  • Published Online:
Keyword(s): MALDI-TOF MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65823-0
2009-04-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/4/766.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65823-0&mimeType=html&fmt=ahah

References

  1. Brehm, K., Spiliotis, M., Zavala-Gongora, R., Konrad, C. & Frosch, M.(2006). The molecular mechanisms of larval cestode development: first steps into an unknown world. Parasitol Int 55, S15–S21.[CrossRef] [Google Scholar]
  2. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  3. Devriese, L. A., Kilpper-Bälz, R. & Schleifer, K. H.(1988).Streptococcus hyointestinalis sp. nov. from the gut of swine. Int J Syst Bacteriol 38, 440–441.[CrossRef] [Google Scholar]
  4. Facklam, R.(2002). What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin Microbiol Rev 15, 613–630.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1993).phylip (phylogeny inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, WA, USA.
  6. Friedrichs, C., Rodloff, A. C., Chhatwal, G. S., Schellenberger, W. & Eschrich, K.(2007). Rapid identification of viridans streptococci by mass spectrometric discrimination. J Clin Microbiol 45, 2392–2397.[CrossRef] [Google Scholar]
  7. Goldenberger, D., Künzli, A., Vogt, P., Zbinden, R. & Altwegg, M.(1997). Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing. J Clin Microbiol 35, 2733–2739. [Google Scholar]
  8. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  9. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by N. Munro. New York: Academic Press.
  10. Lawson, P. A., Foster, G., Falsen, E., Markopoulos, S. J. & Collins, M. D.(2005).Streptococcus castoreus sp. nov., isolated from a beaver (Castor fiber). Int J Syst Evol Microbiol 55, 843–846.[CrossRef] [Google Scholar]
  11. Maidak, B. L., Cole, J. R., Lilburn, T. G., Parker, C. T., Jr, Saxman, P. R., Farris, R. J., Garrity, G. M., Olsen, G. J., Schmidt, T. M. & Tiedje, J. M.(2001). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef] [Google Scholar]
  12. Schleifer, K. H.(1985). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156. [Google Scholar]
  13. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  14. Schumann, P.(2007). MALDI-TOF mass spectrometry versus RIBOPRINTING in the quality control of bacterial strains. In Proceedings of the 11th International Conference on Culture Collections, ICCC 11, Goslar, Germany. Edited by E. Stackebrandt, M. Wozniczka, V. Weihs & J. Sikorski. Braunschweig: Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH/World Federation for Culture Collections.
  15. Spiliotis, M., Tappe, D., Sesterhenn, L. & Brehm, K.(2004). Long-term in vitro cultivation of Echinococcus multilocularis metacestodes under axenic conditions. Parasitol Res 92, 430–432.[CrossRef] [Google Scholar]
  16. Stackebrandt, E., Päuker, O. & Erhard, M.(2005). Grouping myxococci (Corallococcus) strains by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry: comparison with gene sequence phylogenies. Curr Microbiol 50, 71–77.[CrossRef] [Google Scholar]
  17. Täpp, J., Thollesson, M. & Herrmann, B.(2003). Phylogenetic relationships and genotyping of the genus Streptococcus by sequence determination of the RNase P RNA gene, rnpB. Int J Syst Evol Microbiol 53, 1861–1871.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65823-0
Loading
Streptococcus merionis sp. nov., isolated from Mongolian jirds (Meriones unguiculatus)
Int J Syst Evol Microbiol 59, 766 (2009); https://doi.org/10.1099/ijs.0.65823-0
/content/journal/ijsem/10.1099/ijs.0.65823-0
/content/journal/ijsem/10.1099/ijs.0.65823-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 766 – 770

Phylogenetic tree based on 16S rRNA gene sequences, showing the relationships among strain WUE3771 and related species of the genus

Score-oriented dendrogram of species related to the type strain of , WUE3771 , and three additional isolates

[ Single PDF file] (218 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error