A Gram-negative, strictly aerobic, non-motile, club-shaped bacterial strain, designated CL-ES2T, was isolated from coastal water from the east coast of Korea. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain CL-ES2T was related to the genera Phaeobacter (95.0–96.6 % similarity to the type strains), Leisingera (96.1 %) and Marinovum (95.6 %) in the family Rhodobacteraceae. However, strain CL-ES2T did not form a robust clade with any species of the Roseobacter clade, instead forming a distinct subline. The optimum temperature and pH for growth were 25 °C and pH 7. Strain CL-ES2T was able to grow with sea salts at concentrations in the range 2–6 %, with optimum growth occurring at 3–4 %. The major fatty acid was C18 : 1ω7c (75.2 %). The polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and three unidentified lipids. The isoprenoid quinone was Q-10. The G+C content of the DNA was 47.0 mol%. On the basis of the data from the polyphasic analysis, strain CL-ES2T represents a novel genus and species, for which the name Pelagicola litoralis gen. nov., sp. nov. is proposed. The type strain of Pelagicola litoralis is CL-ES2T (=KCCM 42274T =DSM 18290T).
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Supplementary Fig. S1. Neighbour-joining
phylogenetic tree, based on 16S rRNA gene sequences, for strain
CL-ES2
Tand most members of the family
Rhodobacteraceae . [
PDF] 71 KB
Supplementary Fig. S2. Polar lipid composition
of strain CL-ES2
T. The TLC plate was sprayed with molybdatophosphoric
acid. AL, Unidentified aminolipid; L1–L3, unidentified
lipids; PC, phosphatidylcholine; PE, phosphatidylethanolamine;
PG, phosphatidylglycerol.
Supplementary Fig. S3. Cells of strain CL-ES2
Tin exponential phase (a) and stationary phase (d)
stained with DAPI. [
PDF] 56 KB
Supplementary Table S1. Whole-cell fatty acid
compositions of strain CL-ES2
Tand members of the genera
Phaeobacter ,
Leisingera and
Marinovum . [
PDF] 42 KB