gen. nov., sp. nov., an alkane-degrading bacterium isolated from Semarang Port in Indonesia Free

Abstract

Strain A34, an obligately halophilic, Gram-negative, non-motile, rod-shaped bacterium, was isolated from seawater obtained from Semarang Port in Indonesia. It possesses a pink pigment and degrades short-chain alkanes. It is positive for catalase and oxidase and reduces nitrate to nitrite. Analyses of 16S rRNA gene sequences revealed a clear affiliation of this strain with the family ‘’ in the class , with its closest relatives being A3 (94.9 % sequence similarity) and B33 (93.4 %). The DNA G+C content was 69.1 mol%. The major cellular fatty acids of strain A34 were C 7 (56.2 %), C cyclo 8 (26.0 %) and C (9.1 %), while the predominant respiratory lipoquinone was ubiquinone-10. Based on the physiological and phylogenetic data, it is proposed that strain A34 should be classified in a new genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is strain A34 (=JCM 14836 =DSM 19547).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65817-0
2008-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/9/2118.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65817-0&mimeType=html&fmt=ahah

References

  1. Beveridge, T. J., Popkin, T. J. & Cole, R. M.(1994). Electron microscopy. In Methods for General and Molecular Bacteriology, pp. 42–71. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  2. Chaillan, F., Le Fleche, A., Bury, E., Phantavong, Y. H., Grimont, P., Saliot, A. & Oudot, J.(2004). Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms. Res Microbiol 155, 587–595.[CrossRef] [Google Scholar]
  3. de Lima, T. C. S., Grisi, B. M. & Bonato, M. C. M.(1999). Bacteria isolated from a sugarcane agroecosystem: their potential production of polyhydroxyalcanoates and resistance to antibiotics. Rev Microbiol 30, 214–224.[CrossRef] [Google Scholar]
  4. Harwati, T. U., Kasai, Y., Kodama, Y., Susilaningsih, D. & Watanabe, K.(2007). Characterization of diverse hydrocarbon-degrading bacteria isolated from Indonesian seawater. Microbes Environ 22, 412–415.[CrossRef] [Google Scholar]
  5. Kasai, Y., Kishira, H. & Harayama, S.(2002a). Bacteria belonging to the genus Cycloclasticus play a primary role in the degradation of aromatic hydrocarbons released in a marine environment. Appl Environ Microbiol 68, 5625–5633.[CrossRef] [Google Scholar]
  6. Kasai, Y., Kishira, H., Sasaki, T., Syutsubo, K., Watanabe, K. & Harayama, S.(2002b). Predominant growth of Alkanivorax strains in oil-contaminated nutrient supplemented seawater. Environ Microbiol 4, 141–147.[CrossRef] [Google Scholar]
  7. Katayama-Fujimura, Y., Komatsu, Y., Kuraishi, H. & Kaneko, T.(1984). Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 48, 3169–3172.[CrossRef] [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  9. Maidak, B. L., Cole, J. R., Parker, C. T., Jr, Garrity, G. M., Larsen, N., Li, B., Lilburn, T. G., McCaughey, M. J., Olsen, G. J. & other authors(1999). A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27, 171–173.[CrossRef] [Google Scholar]
  10. Malins, D. C., Krahn, M. M., Brown, D. W., Rhodes, L. D., Myers, M. S., McCain, B. B. & Chan, S. L.(1985). Toxic chemicals in marine sediment and biota from Mukilteo, Washington: relationships with hepatic neoplasms and other hepatic lesions in English sole (Parophrys vetulus). J Natl Cancer Inst 74, 487–494. [Google Scholar]
  11. Martínez-Cánovas, M. J., Quesada, E., Martínez-Checa, F., del Moral, A. & Béjar, V.(2004).Salipiger mucescens gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from hypersaline soil, belonging to the α-Proteobacteria. Int J Syst Evol Microbiol 54, 1735–1740.[CrossRef] [Google Scholar]
  12. Martínez-Checa, F., Quesada, E., Martínez-Cánovas, M. J., Llamas, I. & Béjar, V.(2005).Palleronia marisminoris gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium belonging to the ‘Alphaproteobacteria’, isolated from a saline soil. Int J Syst Evol Microbiol 55, 2525–2530.[CrossRef] [Google Scholar]
  13. Meador, J. P., Stein, J. E., Reichert, W. L. & Varanasi, U.(1995). Bioaccumulation of polycyclic aromatic hydrocarbons by marine organisms. Rev Environ Contam Toxicol 143, 79–165. [Google Scholar]
  14. Nishimura, Y., Muroga, Y., Saito, S., Shiba, T., Takamiya, K. & Shioi, Y.(1994). DNA relatedness and chemotaxonomic features of aerobic bacteriochlorophyll-containing bacteria isolated from coasts of Australia. J Gen Appl Microbiol 40, 287–296.[CrossRef] [Google Scholar]
  15. Ozaki, S., Kishimoto, N. & Fujita, T.(2006). Isolation and phylogenetic characterization of microbial consortia able to degrade aromatic hydrocarbons at high rates. Microbes Environ 21, 44–52.[CrossRef] [Google Scholar]
  16. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  17. Sohn, J. H., Kwon, K. K., Kang, J.-H., Jung, H.-B. & Kim, S.-J.(2004).Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54, 1483–1487.[CrossRef] [Google Scholar]
  18. Suzuki, T., Muroga, Y., Takahama, M. & Nishimura, Y.(1999).Roseivivax halodurans gen. nov., sp. nov., and Roseivivax halotolerans sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from a saline lake. Int J Syst Bacteriol 49, 629–634.[CrossRef] [Google Scholar]
  19. Thompson, J. D., Gibson, T. J., Plewniak, K., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  20. Zhuang, W.-Q., Tay, J.-H., Maszenan, A. M. & Tay, S. T.-L.(2003). Isolation of naphthalene-degrading bacteria from tropical marine sediments. Water Sci Technol 47 1, 303–308. [Google Scholar]
  21. Zinjarde, S. S. & Pant, A. A.(2002). Hydrocarbon degraders from tropical marine environments. Mar Pollut Bull 44, 118–121.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65817-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65817-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed