A novel moderately halophilic, non-motile, rod-shaped bacterium was isolated from a saline lake, Lake Shangmatala, in the Inner Mongolia Autonomous Region, China. This bacterium, designated SH4s, was strictly aerobic, catalase-positive and oxidase-negative. It grew at salinities of 3–20 % (w/v) NaCl, with an optimum at 10 % (w/v) NaCl. The cell-wall peptidoglycan was of the A4 type, based on -Orn–-Asp, and the major quinone was a menaquinone with seven isoprene units (MK-7). The major fatty acids were iso-C and iso-C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a glycolipid and four different unidentified phospholipids. The DNA G+C content was 45.9 mol%. In a maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences, strain SH4s was found to belong to the family and to be most closely related to members of the genera (95.9 % sequence similarity), (95.7 %) and (95.4 %). DNA–DNA hybridization experiments revealed 10 % relatedness (12 %, reciprocally) between strain SH4s and DSM 13259, the sole species of the genus. All of these data show that strain SH4s represents a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is SH4s (=CCM 7366 =CECT 7149 =DSM 18090).


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Neighbour-joining (S1) and maximum-likelihood (S2) phylogenetic trees, based on 16S rRNA gene sequences, showing the relationships between SH4s and related species. GenBank accession numbers are shown in parentheses. Bars, 0.02 substitutions per nucleotide position. [PDF](20 KB)


Two-dimensional thin-layer chromatogram of the polar lipids of SH4s . DPG, Diphosphatidylglycerol; PE-tr, phosphatidylethanolamine (traces); PG, phosphatidylglycerol; PL, phospholipid; GL, glycolipid.


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