1887

Abstract

The taxonomy of myxobacteria is based mainly on their morphological characteristics. The genus belongs to the myxobacterial suborder . Strains in the genus were classified either as one species, , by ignoring divergent morphological characteristics, or into several species; however, the latter classification is based on some dubious morphological characteristics and is inconsistent with the phylogeny constructed from 16S rRNA gene sequences. In this study, two HSP60 ( and ) genes were amplified and sequenced from 22 strains. The and gene sequences were highly conserved in strains, suggesting that these two paralogous genes both play important roles in the life cycle. The phylogeny constructed by the genes was rather consistent with the morphological characteristics of sporangioles. Including information from the phylogenetic analysis and morphological characteristics, it is suggested that the genus includes two species.

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2008-11-01
2024-03-29
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References

  1. Dahl, J. L., Tengra, F. K., Dutton, D., Yan, J., Andacht, T. M., Coyne, L., Windell, V. & Garza, A. G.(2007). Identification of major sporulation proteins of Myxococcus xanthus using a proteomic approach. J Bacteriol 189, 3187–3197.[CrossRef] [Google Scholar]
  2. Felsenstein, J.(2001).phylip (phylogeny inference package) version 3.61. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  3. Gerth, K., Pradella, S., Perlova, O., Beyer, S. & Muller, R.(2003). Myxobacteria: proficient producers of novel natural products with various biological activities – past and future biotechnological aspects with the focus on the genus Sorangium. J Biotechnol 106, 233–253.[CrossRef] [Google Scholar]
  4. Goh, S. H., Potter, S., Wood, J. O., Hemmingsen, S. M., Reynolds, R. P. & Chow, A. W.(1996). HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci. J Clin Microbiol 34, 818–823. [Google Scholar]
  5. Goyal, K., Qamra, R. & Mande, S. C.(2006). Multiple gene duplication and rapid evolution in the groEL gene: functional implications. J Mol Evol 63, 781–787.[CrossRef] [Google Scholar]
  6. Hou, P. B., Li, Y. Z., Wu, B. H., Yan, Z. C., Yan, B. X. & Gao, P. J.(2006). Cellulolytic complex exists in cellulolytic myxobacterium Sorangium. Enzyme Microb Technol 38, 273–278.[CrossRef] [Google Scholar]
  7. Karlin, S. & Brocchieri, L.(2000). Heat shock protein 60 sequence comparisons: duplications, lateral transfer, and mitochondrial evolution. Proc Natl Acad Sci U S A 97, 11348–11353.[CrossRef] [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  9. Li, Y. Z., Hu, W., Zhang, Y. Q., Qiu, Z., Zhang, Y. & Wu, B. H.(2002). A simple method to isolate salt-tolerant myxobacteria from marine samples. J Microbiol Methods 50, 205–209.[CrossRef] [Google Scholar]
  10. Lin, Z. & Rye, H. S.(2006). GroEL-mediated protein folding: making the impossible, possible. Crit Rev Biochem Mol Biol 41, 211–239.[CrossRef] [Google Scholar]
  11. McCurdy, H. D.(1989). Order Myxococcales Tchan, Pochon and Prévot 1948, 398AL. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2139–2144. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  12. Otani, M., Tabata, J., Ueki, T., Sano, K. & Inouye, S.(2001). Heat-shock-induced proteins from Myxococcus xanthus. J Bacteriol 183, 6282–6287.[CrossRef] [Google Scholar]
  13. Reichenbach, H.(2005). Order VIII. Myxococcales Tchan, Pochon and Prévot 1948, 398AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 1059–1072. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  14. Reichenbach, H. & Dworkin, M.(1992). The myxobacteria. In The Prokaryotes, 2nd edn, pp. 3416–3487. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  15. Sambrook, J. & Russell, D. W.(2001).Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  16. Schneiker, S., Perlova, O., Kaiser, O., Gerth, K., Alici, A., Altmeyer, M. O., Bartels, D., Bekel, T., Beyer, S. & other authors(2007). Complete genome sequence of the myxobacterium Sorangium cellulosum. Nat Biotechnol 25, 1281–1289.[CrossRef] [Google Scholar]
  17. Shimkets, L. J., Dworkin, M. & Reichenbach, H.(2006). The myxobacteria. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 31–115. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  19. Yan, Z. C., Wang, B., Li, Y. Z., Gong, X., Zhang, H. Q. & Gao, P. J.(2003). Morphologies and phylogenetic classification of cellulolytic myxobacteria. Syst Appl Microbiol 26, 104–109.[CrossRef] [Google Scholar]
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Typical morphologies of sporangioles and swarms in different strains. (a) Spherical sporangioles packed in yellow fruiting bodies on the whole swarm of So0133-4. (b) Spherical sporangioles packed in orange fruiting bodies at the swarm edge of So9881. (c) Spherical sporangioles packed in black fruiting bodies on the whole swarm of So0089-1. (d) Scattered fruiting bodies embedding spherical sporangioles on the swarm of So0157-52. (e) Polyhedral sporangioles packed in deep-brown fruiting bodies at the centre of swarms of So9741. (f) Polyhedral sporangioles packed in deep-brown fruiting bodies on the whole swarm of DSM 14731. (g) Unpacked spherical sporangioles scattered in swarms of So0139-5. (h) Small extension of swarms of So0157-24 from filter paper. (i) Large extension of swarms of So0184-1 from filter paper. Bars, 1 mm (a) [applies to (a)–(f), (h) and (i)] and 50 µm (inset in a) [applies to insets of (a)-(f) and to (g)].

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Multiple alignment of 16S rRNA gene sequences. The open and filled boxes respectively represent conserved and variable regions of bacterial 16S rRNA gene sequences (Neefs , 1991). Numbers below the boxes indicate the positions of 16S rRNA gene sequences, labelled according to the sequence numbers of the 16S rRNA sequence in (Neefs , 1991). Divergent nucleotides are highlighted.

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