@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65799-0, author = "Nedashkovskaya, Olga I. and Kwon, Kae Kyoung and Yang, Sung-Hyun and Lee, Hee-Soon and Chung, Kyung Ho and Kim, Sang-Jin", title = "Lacinutrix algicola sp. nov. and Lacinutrix mariniflava sp. nov., two novel marine alga-associated bacteria and emended description of the genus Lacinutrix", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "12", pages = "2694-2698", doi = "https://doi.org/10.1099/ijs.0.65799-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65799-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two heterotrophic, aerobic, yellow-pigmented, Gram-negative, non-gliding bacteria, designated AKS293T and AKS432T, isolated from a red alga, were analysed using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed that the novel strains were affiliated to the genus Lacinutrix, a member of the family Flavobacteriaceae, showing sequence similarities of 96.1–96.4 % with respect to the type strain of Lacinutrix copepodicola. The two novel isolates shared 99.5 % 16S rRNA gene sequence similarity and 55.0 % DNA–DNA relatedness. They grew optimally at 17.5 °C and pH 6.5. The main cellular fatty acids of strain AKS293T were iso-C15 : 0, iso-C15 : 0 3-OH and iso-C16 : 0 3-OH, while those of strain AKS432T were anteiso-C15 : 0, iso-C15 : 0, iso-C15 : 1 and iso-C15 : 0 3-OH. In both cases, the major isoprenoid quinone was MK-6. The DNA G+C contents were 34.7 and 37.0 mol% for strains AKS293T and AKS432T, respectively. The phylogenetic evidence, phenotypic data and DNA–DNA hybridization results support the differentiation of strains AKS293T and AKS432T from each other and from their closest relative, L. copepodicola DJ3T. Therefore, strains AKS293T and AKS432T represent two novel species, for which the names Lacinutrix algicola sp. nov. and Lacinutrix mariniflava sp. nov. are proposed, respectively. The type strain of L. algicola sp. nov. is AKS293T (=KCCM 42313T=JCM 13825T) and the type strain of L. mariniflava sp. nov. is AKS432T (=KCCM 42306T=JCM 13824T). An emended description of the genus Lacinutrix is also proposed.", }