1887

Abstract

A bacterial isolate, strain XM415, obtained from soil of Tibet in China was characterized using a polyphasic taxonomic approach. Strain XM415 was aerobic, Gram-negative, gliding, rod-shaped, oxidase-negative and catalase-positive. Strain XM415 showed the highest 16S rRNA gene sequence similarity with GH41-7 (96.0 %). Ubiquinone Q-8 and branched fatty acids, such as iso-C (24.0 %), iso-C (22.6 %), iso-C 9 (6.7 %), iso-C (6.1 %) and iso-C 3-OH (5.2 %), were predominant in strain XM415 as well as in all type strains of recognized species. The DNA G+C content of XM415 was 63.5 mol%. The genotypic and phenotypic data show that strain XM415 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XM415 (=CCTCC AB 207091 =NRRL B-51263).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65796-0
2009-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/4/786.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65796-0&mimeType=html&fmt=ahah

References

  1. Assih, E. A., Ouattara, A. S., Thierry, S., Cayol, J.-L., Labat, M. & Macarie, H.(2002).Stenotrophomonas acidaminiphila sp. nov., a strictly aerobic bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor. Int J Syst Evol Microbiol 52, 559–568. [Google Scholar]
  2. Bae, H.-S., Im, W.-T. & Lee, S.-T.(2005).Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol 55, 1155–1161.[CrossRef] [Google Scholar]
  3. Bowman, J. P.(2000). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868. [Google Scholar]
  4. Christensen, P.(2005). Genus IV. Lysobacter Christensen and Cook 1978, 372AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 95–101. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  5. Christensen, P. & Cook, F. D.(1978).Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. Int J Syst Bacteriol 28, 367–393.[CrossRef] [Google Scholar]
  6. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  7. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  8. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  9. Kimura, H., Miyashita, H. & Sumino, Y.(1996). Organization and expression in Pseudomonas putida of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90. Appl Microbiol Biotechnol 45, 490–501. [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  11. Lee, J. W., Im, W.-T., Kim, M. K. & Yang, D.-C.(2006).Lysobacter koreensis sp. nov., isolated from a ginseng field. Int J Syst Evol Microbiol 56, 231–235.[CrossRef] [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  13. Ono, H., Nozaki, Y., Katayama, N. & Okazaki, H.(1984). Cephabacins, new cephem antibiotics of bacterial origin. I. Discovery and taxonomy of the producing organisms and fermentation. J Antibiot (Tokyo) 37, 1528–1535.[CrossRef] [Google Scholar]
  14. Park, J. H., Kim, R., Aslam, Z., Jeon, C. O. & Chung, Y. R.(2008).Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter. Int J Syst Evol Microbiol 58, 387–392.[CrossRef] [Google Scholar]
  15. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  16. Romanenko, L. A., Uchino, M., Tanaka, N., Frolova, G. M. & Mikhailov, V. V.(2008).Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge. Int J Syst Evol Microbiol 58, 370–374.[CrossRef] [Google Scholar]
  17. Roumagnac, P., Gagnevin, L., Gardan, L., Sutra, L., Manceau, C., Dickstein, E. R., Jones, J. B., Rott, P. & Pruvost, O.(2004). Polyphasic characterization of xanthomonads isolated from onion, garlic and Welsh onion (Allium spp.) and their relatedness to different Xanthomonas species. Int J Syst Evol Microbiol 54, 15–24.[CrossRef] [Google Scholar]
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Woods & N. R. Krieg. Washington, DC: American Society for Microbiology.
  20. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  22. Weon, H.-Y., Kim, B.-Y., Baek, Y.-K., Yoo, S.-H., Kwon, S.-W., Stackebrandt, E. & Go, S.-J.(2006). Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. Int J Syst Evol Microbiol 56, 947–951.[CrossRef] [Google Scholar]
  23. Weon, H.-Y., Kim, B.-Y., Kim, M.-K., Yoo, S.-H., Kwon, S.-W., Go, S.-J. & Stackebrandt, E.(2007).Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea. Int J Syst Evol Microbiol 57, 548–551.[CrossRef] [Google Scholar]
  24. Xie, C. H. & Yokota, A.(2003). Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef] [Google Scholar]
  25. Yang, D.-C., Im, W.-T., Kim, M. K. & Lee, S.-T.(2005).Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov. Int J Syst Evol Microbiol 55, 787–791.[CrossRef] [Google Scholar]
  26. Yassin, A. F., Chen, W.-M., Hupfer, H., Siering, C., Kroppenstedt, R. M., Arun, A. B., Lai, W.-A., Shen, F.-T., Rekha, P. D. & Young, C. C.(2007).Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 57, 1131–1136.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65796-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65796-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error