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Leptothrix cholodnii is a sheathed bacterium often found in metal-rich and oligotrophic aquatic environments. A bacterial strain that is able to degrade the NaOH-treated sheath of L. cholodnii was isolated. The isolate was a Gram-negative, aerobic and prosthecate bacterium. The optimum growth temperature and pH were 30 °C and pH 7.0, respectively. The DNA G+C content was 62.9 mol%. The major respiratory quinone was MK-6. A phylogenetic analysis based on the 16S rRNA gene indicated that the isolate is a member of the genus Prosthecobacter. The nearest relative was the type strain of Prosthecobacter vanneervenii, with a similarity of 97.1 %. However, the isolate does not possess the bacterial tubulin genes, btubA and btubB, unique to known species of the genus Prosthecobacter. It is proposed that the isolate represents a novel species, Prosthecobacter fluviatilis sp. nov. The type strain is HAQ-1T (=JCM 14805T =KACC 12649T =KCTC 22182T).
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International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 7, pp. 1561 - 1565
Supplementary Fig. S1. Typical time-course of mucopolysaccharide degradation and growth of strain HAQ-1 T.
Supplementary Fig. S2. Binding positions of PCR primers designed for amplification of the btubA gene.
Supplementary Fig. S3. Binding positions of PCR primers designed for amplification of the btubB gene.
[PDF file of Supplementary Figs S1-S3](83 KB)