Full text loading...
Gram-positive, rod-shaped, non-motile lactic acid bacteria, strains iz4b-1T, iz4b-2 and iz4c-1, were isolated in an attempt to study the composition of the Lactobacillus flora of Japanese pickles. Analysis of their 16S rRNA gene sequences revealed that the strains clustered in the Lactobacillus alimentarius group, and comparatively high similarities were shown to ‘Lactobacillus tucceti’ CECT 5920 (97.0 %), Lactobacillus versmoldensis KU-3T (96.4 %) and Lactobacillus nantensis LP33T (94.4 %). DNA–DNA hybridization assays clearly revealed that the isolates represented a novel taxon. The DNA G+C content was 40.6 mol% and the peptidoglycan type was l-Lys–d-Asp. Thus, these isolates represent a novel Lactobacillus species, for which the name Lactobacillus nodensis sp. nov. is proposed. The type strain is iz4b-1T (=DSM 19682T =JCM 14932T).
Article metrics loading...
Full text loading...
References
Data & Media loading...
Supplements
International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 1, pp. 83 - 86
Supplementary Figs S1 and S2. Phylogenetic trees showing the relationships between Lactobacillus nodensis sp. nov. iz4b-1 Tand related Lactobacillus species on the basis of 16S rRNA gene sequences, constructed using the maximum-likelihood (S1) and maximum-parsimony (S2) methods. [PDF](666 KB)
Supplementary Table S1. DNA–DNA hybridization between L. nodensis sp. nov. strains iz4b-1 T, iz4b-2 and iz4c-1 and ' L. tucceti ' CECT 5920
In each assay, each combination was tested three times and mean percentages are indicated. –, Not done.