Gram-positive, rod-shaped, non-motile lactic acid bacteria, strains iz4b-1, iz4b-2 and iz4c-1, were isolated in an attempt to study the composition of the flora of Japanese pickles. Analysis of their 16S rRNA gene sequences revealed that the strains clustered in the group, and comparatively high similarities were shown to ‘’ CECT 5920 (97.0 %), KU-3 (96.4 %) and LP33 (94.4 %). DNA–DNA hybridization assays clearly revealed that the isolates represented a novel taxon. The DNA G+C content was 40.6 mol% and the peptidoglycan type was -Lys–-Asp. Thus, these isolates represent a novel species, for which the name sp. nov. is proposed. The type strain is iz4b-1 (=DSM 19682 =JCM 14932).


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vol. , part 1, pp. 83 - 86

Phylogenetic trees showing the relationships between sp. nov. iz4b-1 and related species on the basis of 16S rRNA gene sequences, constructed using the maximum-likelihood (S1) and maximum-parsimony (S2) methods. [PDF](666 KB)

DNA–DNA hybridization between sp. nov. strains iz4b-1 , iz4b-2 and iz4c-1 and ' ' CECT 5920

In each assay, each combination was tested three times and mean percentages are indicated. –, Not done.


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