Ascogenous yeasts were isolated from a decaying mangosteen fruit ( L., Clusiaceae). Based on colony morphology and RAPD analysis, the strains were grouped into four groups (G-I, G-II, G-III and G-IV). Phenotypic properties and analysis of the D1/D2 domain sequence of the 26S rRNA gene identified representative members of groups G-II, G-III and G-IV as (=), and , respectively. Representatives of group G-I, YS110 and YS111, showed similar phenotypic traits, 99.9 % similarity in the sequence of the D1/D2 domain of the 26S rRNA gene and ITS1–5.8S rRNA gene–ITS2 sequence and 92 % DNA–DNA relatedness. Hence, YS110 and YS111 are regarded as members of the same species. Based on D1/D2 domain and ITS region sequences, the nearest phylogenetic neighbours of YS110 and YS111 were identified as and . However, YS110 differs from these two nearest phylogenetic neighbours by >3 % in the D1/D2 domain sequence and by >18 % with respect to the ITS region sequence. In addition, YS110 and YS111 differ from NRRL Y-2026 and NRRL Y-27978 with respect to a number of phenotypic traits. The strains show 52–57 % DNA–DNA relatedness with NRRL Y-2026 and NRRL Y-27978. Strains YS110 and YS111 are proposed as two strains of a novel species, for which the name sp. nov. is proposed. The type strain is YS110 (=NRRL Y-48422 =CBS 10758).


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vol. , part 11, pp. 2665 - 2669

Agarose gel electrophoresis of RAPD profiles from 12 novel yeast isolates from rotting mangosteen fruit generated using the (GTG) primer. Lanes: 2–4, representatives of group G-I; 5–7, representatives of G-II; 8–10, representatives of G-III; 11–13, representatives of G-IV; 1 and 14, molecular size markers.

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