An aerobic, Gram-negative bacterial isolate, strain DSM 19503, was isolated from haemolymph serum of the blacklip abalone . Cells of strain DSM 19503 were vibrioid to spiral, motile and were able to pass through sterile filters with a pore size of 0.2 μm, indicating the small width of the bacterium. The isolate was psychrophilic, with the ability to grow at 2–8 °C. Oxidase activity was present, whereas catalase activity was absent. The nearly complete 16S rRNA gene sequence of strain DSM 19503 was obtained and phylogenetic sequence analysis showed that it formed a distinct genus in the family with highest sequence similarity of 92.9 % to RB-8. The cellular fatty acid composition was dependent on the growth medium used for cultivation. During growth on seawater agar, the fatty acid composition was most similar to that of DSM 14852, with mainly C (90.3 %). In contrast, Columbia blood agar/NaCl-grown cells exhibited mainly C 3-OH (11.8 %), C 5 (8.2 %), C 9 (29.6 %), C (19.3 %) and C 9 (13.1 %) fatty acids. On the basis of phenotypic, chemotaxonomic and genotypic data, strain DSM 19503 is considered to represent a novel species in a new genus for which the name gen. nov., sp. nov. is proposed. The type strain of is DSM 19503 (=LMG 24225).


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  1. Allgaier, M., Uphoff, H., Felske, A. & Wagner-Döbler, I.(2003). Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. Appl Environ Microbiol 69, 5051–5059.[CrossRef] [Google Scholar]
  2. Connon, S. A. & Giovannoni, S. J.(2002). High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl Environ Microbiol 68, 3878–3885.[CrossRef] [Google Scholar]
  3. Hayashi, K., Moriwaki, J., Sawabe, T., Thompson, F. L., Swings, J., Gudkovs, N., Christen, R. & Ezura, Y.(2003).Vibrio superstes sp. nov., isolated from the gut of Australian abalones Haliotis laevigata and Haliotis rubra. Int J Syst Evol Microbiol 53, 1813–1817.[CrossRef] [Google Scholar]
  4. IUPAC-IUB Commission on Biochemical Nomenclature(1977). The nomenclature of lipids (recommendations 1976). Eur J Biochem 79, 11–21.[CrossRef] [Google Scholar]
  5. Lipski, A. & Altendorf, K.(1997). Identification of heterotrophic bacteria isolated from ammonia-supplied experimental biofilters. Syst Appl Microbiol 20, 448–457.[CrossRef] [Google Scholar]
  6. Maidak, B. L., Cole, J. R., Lilburn, T. G. & Parker, C. T., Jr,Saxman, P. R., Farris, R. J., Garrity, G. M., Li, B., Olsen, G. J., Schmidt, T. M. & Tiedje, J. M.(2001). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef] [Google Scholar]
  7. Pearson, W. R.(1990). Rapid and sensitive sequence comparison with fastp and fasta. Methods Enzymol 183, 63–98. [Google Scholar]
  8. Sasser, M.(1990). Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology, pp. 199–204. Edited by Z. Klement, K. Rudolph & D. C. Sands. Budapest: Akademiai Kiado.
  9. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  10. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]

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