1887

Abstract

A transparent, non-pigmented, Gram-negative, rod-shaped bacterium, designated strain DS-28, was isolated from water samples collected from a spring located in Tainan County, Taiwan. 16S rRNA gene sequence analysis indicated that the novel strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus ; the only sole close neighbour of the novel strain was ATCC 19706 (97.4 % 16S rRNA gene sequence similarity). The isolate was distinguished from on the basis of genotypic data, several phenotypic properties and an inability to produce characteristic blue-pigmented colonies on peptone agar. The fatty acid profile was slightly different from that reported for ATCC 19706. It was evident from the genotypic and phenotypic data that strain DS-28 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-28 (=BCRC 17730=LMG 24214).

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2008-12-01
2019-10-14
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References

  1. Chen, W. M., Laevens, S., Lee, T. M., Coenye, T., de Vos, P., Mergeay, M. & Vandamme, P. ( 2001; ). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51, 1729–1735.[CrossRef]
    [Google Scholar]
  2. Chung, Y. C., Kobayashi, T., Kanai, H., Akiba, T. & Kudo, T. ( 1995; ). Purification and properties of extracellular amylase from the hyperthermophilic archaeon Thermococccus profundus DT5432. Appl Environ Microbiol 61, 1502–1506.
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  5. Gillis, M. & Logan, N. A. ( 2005; ). Genus IV. Chromobacterium Bergonzini 1881, 153AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 824–827. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  6. Grimes, D. J., Woese, C. R., MacDonell, M. T. & Colwell, R. R. ( 1997; ). Systematic study of the genus Vogesella gen. nov. and its type species, Vogesella indigofera comb. nov. Int J Syst Bacteriol 47, 19–27.[CrossRef]
    [Google Scholar]
  7. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  8. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  9. Kluge, A. G. & Farris, F. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  12. Lau, H.-T., Faryna, J. & Triplett, E. W. ( 2006; ). Aquitalea magnusonii gen. nov., sp. nov., a novel Gram-negative bacterium isolated from a humic lake. Int J Syst Evol Microbiol 56, 867–871.[CrossRef]
    [Google Scholar]
  13. MacFaddin, J. F. ( 2000; ). Biochemical Tests for the Identification of Medical Bacteria, 3rd edn. Baltimore, MD: Williams & Wilkins.
  14. Martin, P. A. W., Gundersen-Rindal, D., Blackburn, M. & Buyer, J. ( 2007; ). Chromobacterium subtsugae sp. nov., a betaproteobacterium toxic to Colorado potato beetle and other insect pests. Int J Syst Evol Microbiol 57, 993–999.[CrossRef]
    [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Powers, E. M. ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61, 3756–3758.
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  20. Vaz-Moreira, I., Nobre, M. F., Nunes, O. C. & Manaia, C. M. ( 2007; ). Gulbenkiania mobilis gen. nov., sp. nov., isolated from treated municipal wastewater. Int J Syst Evol Microbiol 57, 1108–1112.[CrossRef]
    [Google Scholar]
  21. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches of bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
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Some genotypic and phenotypic characteristics that distinguish strain DS28 from other closely related phylogenetic neighbours. [ PDF] 87 KB

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Colonies of strain DS-28 on R2A agar at 25°C after 48 h. [ PDF] 42 KB

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