1887

Abstract

A bacterial strain, Y8, capable of oxidizing carbon monoxide, was isolated from a soil sample collected from a roadside in Seoul, Korea. On the basis of 16S rRNA gene sequence similarity analyses, strain Y8 was shown to belong to the genus and was related most closely to the type strain of (99.6 % similarity). The cells were aerobic and stained Gram-positive, with white aerial mycelium and brown substrate mycelium. The predominant fatty acids were 16 : 0 iso and 16 : 1 iso. The cell-wall peptidoglycan of strain Y8 contained -diaminopimelic acid. The DNA G+C content was 77 mol%. Strain Y8 contained MK-9 as the major menaquinone, which is different from the major menaquinone reported previously in the genus , MK-8(H). DNA–DNA relatedness between strain Y8 and the type strains of and was respectively 10 and 63 %. Based on phylogenetic, morphological and chemotaxonomic evidence, it is proposed that strain Y8 (=KCCM 42678 =JCM 14827) be classified as the type strain of a novel species, sp. nov. An emended description of the genus is also presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65765-0
2008-11-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/11/2475.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65765-0&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. ( 1995; ). Current Protocols in Molecular Biology. New York: Wiley.
  2. Bedard, C. & Knowles, R. ( 1989; ). Physiology, biochemistry, and specific inhibitors of CH4, NH4, and CO oxidation by methanotrophs and nitrifiers. Microbiol Rev 53, 68–84.
    [Google Scholar]
  3. Chappelle, E. W. ( 1962; ). Carbon monoxide oxidation by algae. Biochim Biophys Acta 62, 45–62.[CrossRef]
    [Google Scholar]
  4. Conrad, R. ( 1996; ). Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO). Microbiol Rev 60, 609–640.
    [Google Scholar]
  5. Embley, T. M., Smida, J. & Stackebrandt, E. ( 1988; ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and the description of Pseudonocardiaceae fam. nov. Syst Appl Microbiol 11, 44–52.[CrossRef]
    [Google Scholar]
  6. Ferry, J. G. ( 1995; ). CO dehydrogenase. Annu Rev Microbiol 49, 305–333.[CrossRef]
    [Google Scholar]
  7. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  8. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  9. Hardy, K. R. & King, G. M. ( 2001; ). Enrichment of high-affinity CO oxidizers in Maine forest soil. Appl Environ Microbiol 67, 3671–3676.[CrossRef]
    [Google Scholar]
  10. Henssen, A. ( 1957; ). Beiträge zur Morphologie und Systematik der thermophilen Actinomyceten. Arch Mikrobiol 26, 373–414 (in German).[CrossRef]
    [Google Scholar]
  11. Holmes, N. S., Morawska, L., Mengersen, K. & Jayaratne, E. R. ( 2005; ). Spatial distribution of submicrometre particles and CO in an urban microscale environment. Atmos Environ 39, 3977–3988.[CrossRef]
    [Google Scholar]
  12. Huang, Y., Wang, L., Lu, Z., Hong, L., Liu, Z., Tan, G. Y. A. & Goodfellow, M. ( 2002; ). Proposal to combine the genera Actinobispora and Pseudonocardia in an emended genus Pseudonocardia, and description of Pseudonocardia zijingensis sp. nov. Int J Syst Evol Microbiol 52, 977–982.[CrossRef]
    [Google Scholar]
  13. Inman, R. E. & Ingersoll, R. B. ( 1971; ). Uptake of carbon monoxide by soil fungi. J Air Pollut Control Assoc 21, 646–647.[CrossRef]
    [Google Scholar]
  14. Kim, Y. M. & Hegeman, G. D. ( 1981; ). Purification and some properties of carbon monoxide dehydrogenase from Pseudomonas carboxydohydrogena. J Bacteriol 148, 904–911.
    [Google Scholar]
  15. Kim, Y. M. & Hegeman, G. D. ( 1983; ). Oxidation of carbon monoxide by bacteria. Int Rev Cytol 81, 1–32.
    [Google Scholar]
  16. Kraut, M., Hugendieck, I., Herwig, S. & Meyer, O. ( 1989; ). Homology and distribution of CO dehydrogenase structural genes in carboxydotrophic bacteria. Arch Microbiol 152, 335–341.[CrossRef]
    [Google Scholar]
  17. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  18. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  19. Lee, S. D., Kim, E. S., Min, K.-L., Lee, W. Y., Kang, S.-O. & Hah, Y. C. ( 2001; ). Pseudonocardia kongjuensis sp. nov., isolated from a gold mine cave. Int J Syst Evol Microbiol 51, 1505–1510.
    [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  21. Meyer, O. & Schlegel, H. G. ( 1983; ). Biology of aerobic carbon monoxide oxidizing bacteria. Annu Rev Microbiol 37, 277–310.[CrossRef]
    [Google Scholar]
  22. Nicholas, R. A., Khan, L. A., Houshaymi, B., Miles, R. J., Ayling, R. D., Hotzel, H. & Sachse, K. ( 2002; ). Close genetic and phenotypic relatedness between Mycoplasma ovine/caprine serogroup 11 and Mycoplasma bovigenitalium. Syst Appl Microbiol 25, 396–402.[CrossRef]
    [Google Scholar]
  23. Novelli, P. C., Masarie, K. A., Tans, P. P. & Lang, P. M. ( 1994; ). Recent changes in atmospheric carbon monoxide. Science 263, 1587–1590.[CrossRef]
    [Google Scholar]
  24. Prabahar, V., Dube, S., Reddy, G. S. N. & Shivaji, S. ( 2004; ). Pseudonocardia antarctica sp. nov. an Actinomycetes from McMurdo Dry Valleys, Antarctica. Syst Appl Microbiol 27, 66–71.[CrossRef]
    [Google Scholar]
  25. Reichert, K., Lipski, A., Pradella, S., Stackebrandt, E. & Altendorf, K. ( 1998; ). Pseudonocardia asaccharolytica sp. nov. and Pseudonocardia sulfidoxydans sp. nov., two new dimethyl disulfide-degrading actinomycetes and emended description of the genus Pseudonocardia. Int J Syst Bacteriol 48, 441–449.[CrossRef]
    [Google Scholar]
  26. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  27. Samoli, E., Touloumi, G., Schwartz, J., Anderson, H. R., Schindler, C., Forsberg, B., Vigotti, M. A., Vonk, J., Kosnik, M. & other authors ( 2007; ). Short-term effects of carbon monoxide on mortality: an analysis within the APHEA project. Environ Health Perspect 115, 1578–1583.[CrossRef]
    [Google Scholar]
  28. Sasser, M. ( 1990; ). Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology, pp. 199–204. Edited by Z. Klement, K. Rudolph & D. C. Sands. Budapest: Akademiai Kiado.
  29. Sohn, S. H., Oh, S. C. & Yeo, Y.-K. ( 1999; ). Prediction of air pollutants by using an artificial neural network. Korean J Chem Eng 16, 382–387.[CrossRef]
    [Google Scholar]
  30. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to the identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  31. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  32. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  33. Zeikus, J. G., Kerby, R. & Krzycki, J. A. ( 1985; ). Single-carbon chemistry of acetogenic and methanogenic bacteria. Science 227, 1167–1173.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65765-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65765-0
Loading

Data & Media loading...

Supplements

[PDF file of Supplementary Tables S1 and S2](82 KB)

PDF

[PDF file of Supplementary Tables S1 and S2](82 KB)

IMAGE

Scanning electron micrograph of aerial mycelium of strain Y8 grown on Bennett's medium for 14 days at 25 °C. Bar, 2 µm.

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error