Strains JS54-2 and JS63-1 were isolated from seawater at Jeju, Korea. Cells of these strains were Gram-positive, non-motile, non-spore-forming, short rods and formed yellow-pigmented colonies on tryptic soy agar. The strains had chemotaxonomic markers that were consistent with their classification in the genus , i.e. MK-11, MK-12 and MK-10 as the major menaquinones, fatty acids that were predominantly iso- and anteiso-branched, galactose and glucose as the cell-wall sugars, peptidoglycan-type B2 with glycolyl residues and DNA G+C contents of 69.3–69.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that the strains were closely related to DSM 16091 and IFO 16062 (with 97.7 and 97.4 % sequence similarity, respectively) and formed a separate lineage with in the genus . Data from DNA–DNA hybridization and phenotypic analyses supported the conclusion that strains JS54-2 and JS63-1 represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JS54-2 (=KCTC 19124 =DSM 19713).


Article metrics loading...

Loading full text...

Full text loading...



  1. Bakir, M. A., Kudo, T. & Benno, Y.(2008).Microbacterium hatanonis sp. nov., isolated as a contaminant of hairspray. Int J Syst Evol Microbiol 58, 654–658.[CrossRef] [Google Scholar]
  2. Bates, R. G. & Bower, V. E.(1956). Alkaline solutions for pH control. Anal Chem 28, 1322–1324.[CrossRef] [Google Scholar]
  3. Behrendt, U., Ulrich, A. & Schumann, P.(2001). Description of Microbacterium foliorum sp. nov. and Microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of Aureobacterium resistens (Funke et al. 1998) as Microbacterium resistens comb. nov. Int J Syst Evol Microbiol 51, 1267–1276. [Google Scholar]
  4. Collins, M. D., Jones, D. & Kroppenstedt, R. M.(1983). Reclassification of Brevibacterium imperiale (Steinhaus) and “Corynebacterium laevaniformans” (Dias and Bhat) in a redefined genus Microbacterium (Orla-Jensen), as Microbacterium imperiale comb. nov. and Microbacterium laevaniformans nom. rev., comb. nov. Syst Appl Microbiol 4, 65–78.[CrossRef] [Google Scholar]
  5. De Soete, G.(1983). A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48, 621–626.[CrossRef] [Google Scholar]
  6. Evtushenko, L. I. & Takeuchi, M.(2006). The family Microbacteriaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 3, pp. 1020–1098. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  9. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  10. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  11. Gomori, G.(1955). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146. [Google Scholar]
  12. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  13. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  14. Klatte, S., Rainey, F. A. & Kroppenstedt, R. M.(1994). Transfer of Rhodococcus aichiensis Tsukamura 1982 and Nocardia amarae Lechevalier & Lechevalier 1974 to the genus Gordona as Gordona aichiensis comb. nov. and Gordona amarae comb. nov. Int J Syst Bacteriol 44, 769–773.[CrossRef] [Google Scholar]
  15. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  16. Laffineur, K., Avesani, V., Cornu, G., Charlier, J., Janssens, M., Wauters, G. & Delmée, M.(2003). Bacteremia due to a novel Microbacterium species in a patient with leukemia and description of Microbacterium paraoxydans sp. nov. J Clin Microbiol 41, 2242–2246.[CrossRef] [Google Scholar]
  17. Lee, J.-S., Lee, K. C. & Park, Y.-H.(2006).Microbacterium koreense sp. nov., from sea water in the South Sea of Korea. Int J Syst Evol Microbiol 56, 423–427.[CrossRef] [Google Scholar]
  18. Minnikin, D. E., Patel, V., Alshamaony, L. & Goodfellow, M.(1977). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27, 104–117.[CrossRef] [Google Scholar]
  19. Orla-Jensen, S.(1919).The Lactic Acid Bacteria. Copenhagen: Host & Sons.
  20. Park, M.-J., Kim, M. K., Kim, H.-B., Im, W.-T., Yi, T.-H., Soung, N.-K. & Yang, D.-C.(2008).Microbacterium ginsengisoli sp. nov., a β-glucosidase-producing bacterium isolated from soil of a ginseng field. Int J Syst Evol Microbiol 58, 429–433.[CrossRef] [Google Scholar]
  21. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a district actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method; a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  24. Schippers, A., Bosecker, K., Spröer, C. & Schumann, P.(2005).Microbacterium oleivorans sp. nov. and Microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading Gram-positive bacteria. Int J Syst Evol Microbiol 55, 655–660.[CrossRef] [Google Scholar]
  25. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 34, 407–477. [Google Scholar]
  26. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H.(1996). Isoprenoid quinone profiles of Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69. [Google Scholar]
  27. Shivaji, S., Bhadra, B., Rao, R. S., Chaturvedi, P., Pindi, P. K. & Raghukumar, C.(2007).Microbacterium indicum sp. nov., isolated from a deep-sea sediment sample from the Chagos Trench, Indian Ocean. Int J Syst Evol Microbiol 57, 1819–1822.[CrossRef] [Google Scholar]
  28. Skerman, V. B. K.(1967).A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  29. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  30. Takeuchi, M. & Hatano, K.(1998a). Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. Int J Syst Bacteriol 48, 739–747.[CrossRef] [Google Scholar]
  31. Takeuchi, M. & Hatano, K.(1998b). Proposal of six new species in the genus Microbacterium and transfer of Flavobacterium marinotypicum ZoBell and Upham to the genus Microbacterium as Microbacterium maritypicum comb. nov. Int J Syst Bacteriol 48, 973–982.[CrossRef] [Google Scholar]
  32. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  33. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  34. Uchida, K., Kudo, T., Suzuki, K. & Nagase, T.(1999). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45, 49–56.[CrossRef] [Google Scholar]
  35. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  36. Yokota, A., Takeuchi, M., Sakane, T. & Weiss, N.(1993). Proposal of six new species in the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov. Int J Syst Bacteriol 43, 555–564.[CrossRef] [Google Scholar]

Data & Media loading...


vol. , part 7, pp. 1616 - 1620

Neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, showing the positions of strains JS54-2 and JS63-1 relative to all known species.

DNA–DNA hybridization between strains JS54-2 and JS63-1 and the type strains of closely related species.

[PDF file of Supplementary Fig. S1 and Table S1](33 KB)

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error