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A Gram-negative micro-organism, designated strain B7T, was isolated from tidal flat sediment and subjected to a polyphasic taxonomic study involving morphological, physiological, biochemical and 16S rRNA gene sequence analyses. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain B7T belonged to the genus Paracoccus and was closely related phylogenetically to Paracoccus marcusii MH1T (97.5 % sequence similarity), Paracoccus marinus KKL-A5T (97.5 %), Paracoccus haeundaensis BC74171T (97.3 %), Paracoccus carotinifaciens E-396T (97.3 %), Paracoccus homiensis DD-R11T (97.2 %), Paracoccus seriniphilus MBT-A4T (96.9 %) and other type strains of the genus Paracoccus (95.2–96.7 %). The G+C content of the genomic DNA and the major isoprenoid quinone of the type strain were 62.0 mol% and ubiquinone-10, respectively. The major fatty acid components were C18 : 1 ω7c (68.9 %) and C18 : 0 (18.1 %); this profile, with C18 : 1 ω7c as the predominant fatty acid, was characteristic of members of the genus Paracoccus. The 16S rRNA gene sequence analysis, DNA–DNA hybridization studies and physiological and biochemical tests identified genotypic and phenotypic differences between strain B7T and recognized Paracoccus species. On the basis of these data, therefore, strain B7T represents a novel species of the genus Paracoccus, for which the name Paracoccus aestuarii sp. nov. is proposed. The type strain is B7T (=KCTC 22049T=DSM 19484T=JCM 15119T).
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