sp. nov., isolated from an oil-polluted saline soil Free

Abstract

Strain SLG5B-19, isolated from an oil-polluted saline soil in Gudao in the coastal Shengli Oilfield, eastern China, was Gram-negative with monoprosthecae or bipolar prosthecae and buds on the prosthecal tips. Growth occurred at NaCl concentrations between 0 and 7 % (w/v), at temperatures between 4 and 45 °C, and at pH 6.0–9.0. Strain SLG5B-19 had Q-9 as the major respiratory quinone and unsaturated C 7 as the predominant cellular fatty acid. The G+C content of the genomic DNA was 59.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SLG5B-19 belonged to a clade with the genera and in the class . However, 16S rRNA gene sequence similarities of strain SLG5B-19 to the phylogenetically most closely related strains, i.e. the type strains of and , were 95.8 and 94.5 %, respectively. In addition, the 16S rRNA gene sequence of strain SLG5B-19 had 24 signature nucleotides that were identical to those of the type strain of . Based on phylogenetic analysis of 16S rRNA gene sequences, strain SLG5B-19 could be allocated to the genus . However, distinct phenotypic differences were observed between strain SLG5B-19 and the type strain of . It is therefore proposed that strain SLG5B-19 represents a novel species in the genus , sp. nov. The type strain is SLG5B-19 (=CGMCC 1.6497=LMG 23927).

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2009-02-01
2024-03-29
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References

  1. Barritt, M. M.(1936). The intensification of the Voges-Proskauer reaction by the addition of α-naphthol. J Pathol Bacteriol 42, 441–454.[CrossRef] [Google Scholar]
  2. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F.(1978). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef] [Google Scholar]
  3. Cho, J.-C. & Giovannoni, S. J.(2003).Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53, 1031–1036.[CrossRef] [Google Scholar]
  4. Dong, X.-Z. & Cai, M.-Y.(2001).Determinative Manual for Routine Bacteriology. Beijing: Scientific Press (English translation).
  5. Felsenstein, J.(2004).phylip (phylogeny inference package), version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Gliesche, C., Fesefeldt, A. & Hirsch, P.(2005). Genus I. Hyphomicrobium Stutzer and Hartleb 1898, 76AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, pp. 476–494. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  7. Gu, J., Cai, H., Yu, S. L., Qu, R., Yin, B., Guo, Y. F., Zhao, J. Y. & Wu, X. L.(2007).Marinobacter gudaonensis sp. nov., isolated from an oil-polluted saline soil in a Chinese oilfield. Int J Syst Evol Microbiol 57, 250–254.[CrossRef] [Google Scholar]
  8. Hirsch, P. & Rades-Rohkohl, E.(1983). Microbial diversity in a groundwater aquifer in Northern Germany. Dev Ind Microbiol 24, 183–200. [Google Scholar]
  9. Hirsch, P. & Siebert, J.(1991). Die Vielfalt der Mikroorganismen im hypersalinen Ekho Lake (Vestfold Hills, Ostantarktis). Mitt Kieler Polarforsch 6, 11–14 (in German). [Google Scholar]
  10. Labbé, N., Juteau, P., Parent, S. & Villemur, R.(2003). Bacterial diversity in a marine methanol-fed denitrification reactor at the Montreal Biodome, Canada. Microb Ecol 46, 12–21.[CrossRef] [Google Scholar]
  11. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  12. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  13. Liessens, J.(1993). Removing nitrate with a methylotrophic fluidized bed: microbiological quality. J Am Water Works Assoc 85, 155–161. [Google Scholar]
  14. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  15. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  16. Mata, J. A., Martínez-Cánovas, J., Quesada, E. & Béjar, V.(2002). A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef] [Google Scholar]
  17. Meyers, A. J. & Meyers, C. D.(1986).Hyphomicrobium-mediated sludge bulking in an industrial wastewater treatment system, abstract N-93, p. 257. In Abstracts of the 86th Annual Meeting of the American Society for Microbiology 1986. Washington, DC: American Society for Microbiology.
  18. Morgan, P. & Dow, C. S.(1985). Environmental control of cell-type expression in prosthecate bacteria. In Bacteria in their Natural Environments, pp. 131–169. Edited by M. Fletcher & G. D. Floodgate. London: Academic Press.
  19. Poindexter, J. S.(1992). Dimorphic prosthecate bacteria: the genera Caulobacter, Asticcacaulis, Hyphomicrobium, Pedomicrobium, Hyphomonas, and Thiodendron. In The Prokaryotes, 2nd edn, pp. 2176–2196. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  20. Rainey, F. A., Ward-Rainey, N., Gliesche, C. G. & Stackebrandt, E.(1998). Phylogenetic analysis and intrageneric structure of the genus Hyphomicrobium and the related genus Filomicrobium. Int J Syst Bacteriol 48, 635–639.[CrossRef] [Google Scholar]
  21. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  22. Schlesner, H.(1987).Filomicrobium fusiforme gen. nov., sp. nov., a slender budding, hyphal bacterium from brackish water. Syst Appl Microbiol 10, 63–67.[CrossRef] [Google Scholar]
  23. Sittig, M. & Hirsch, P.(1992). Chemotaxonomic investigation of budding and/or hyphal bacteria. Syst Appl Microbiol 15, 209–222.[CrossRef] [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  25. Stackebrandt, E., Fischer, A., Roggentin, T., Wehmeyer, U., Bomar, D. & Smida, J.(1988). A phylogenetic survey of budding, and/or prosthecate, non-phototrophic eubacteria: membership of Hyphomicrobium, Hyphomonas, Pedomicrobium, Filomicrobium, Caulobacter and ‘Dichotomicrobium’ to the alpha-subdivision of purple non-sulfur bacteria. Arch Microbiol 149, 547–556.[CrossRef] [Google Scholar]
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  27. Urakami, T., Sasaki, J., Suzuki, K.-I. & Komagata, K.(1995). Characterization and description of Hyphomicrobium denitrificans sp. nov. Int J Syst Bacteriol 45, 528–532.[CrossRef] [Google Scholar]
  28. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
  29. Zumft, W. G.(1992). The denitrifying bacteria. In The Prokaryotes, pp. 554–582. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer-Verlag.
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