Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 10

gen. nov., sp. nov., isolated from a cold spring Free

Abstract

A yellow-coloured bacterial strain, designated BP-5, was isolated from a water sample of a cold spring located in the Hsinchu area of northern Taiwan. Cells of the strain were Gram-negative, non-spore-forming rods with a polar flagellum. The isolate was able to grow at 15–42 °C, 0–1 % NaCl and pH 6–8. The predominant fatty acids were summed feature 3 (C 7 and/or iso-C 2-OH), C and C 7. The major respiratory quinone was ubiquinone 8. The DNA G+C content was 63.2 mol%. 16S rRNA gene sequence analysis indicated that strain BP-5 belonged to the class and was a member of the family . Its closest phylogenetic neighbours were E4FC31 (95.0 % 16S rRNA gene sequence similarity), PRAA4-1 (95.0 %), ATCC 12472 (94.3 %), ATCC 19706 (93.8 %) and TRO-001DR8 (93.7 %). On the basis of the evidence from this polyphasic study, strain BP-5 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is BP-5 (=BCRC 17727 =LMG 24211).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65755-0
2008-10-01
2026-04-22

Metrics

Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/10/2384.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65755-0&mimeType=html&fmt=ahah

References

  1. Chen, W. M., Laevens, S., Lee, T. M., Coenye, T., de Vos, P., Mergeay, M. & Vandamme, P.(2001).Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51, 1729–1735.[CrossRef] [Google Scholar]
  2. Chung, Y. C., Kobayashi, T., Kanai, H., Akiba, T. & Kudo, T.(1995). Purification and properties of extracellular amylase from the hyperthermophilic archeon Thermococcus profundus DT5432. Appl Environ Microbiol 61, 1502–1506. [Google Scholar]
  3. Collins, M. D.(1985). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366. [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  5. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  6. Gillis, M. & Logan, N. A.(2005). Genus IV. Chromobacterium Bergonzini 1881, 153AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 824–827. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  7. Grimes, D. J., Woese, C. R., MacDonell, M. T. & Colwell, R. R.(1997). Systematic study of the genus Vogesella gen. nov. and its type species, Vogesella indigofera comb. nov. Int J Syst Bacteriol 47, 19–27.[CrossRef] [Google Scholar]
  8. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  9. Kimura, M.(1983).The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  10. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  12. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  13. Lau, H.-T., Faryna, J. & Triplett, E. W.(2006).Aquitalea magnusonii gen. nov., sp. nov., a novel Gram-negative bacterium isolated from a humic lake. Int J Syst Evol Microbiol 56, 867–871.[CrossRef] [Google Scholar]
  14. MacFaddin, J. F.(2000).Biochemical Tests for the Identification of Medical Bacteria, 3rd edn. Baltimore: Williams & Wilkins.
  15. Martin, P. A. W., Gundersen-Rindal, D., Blackburn, M. & Buyer, J.(2007).Chromobacterium subtsugae sp. nov., a betaproteobacterium toxic to Colorado potato beetle and other insect pests. Int J Syst Evol Microbiol 57, 993–999.[CrossRef] [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  17. Miller, P. H., Wiggs, L. S. & Miller, J. M.(1995). Evaluation of AnaeroGen system for growth of anaerobic bacteria. J Clin Microbiol 33, 2388–2391. [Google Scholar]
  18. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, K. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  19. Powers, E. M.(1995). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61, 3756–3758. [Google Scholar]
  20. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  21. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  23. Vaz-Moreira, I., Nobre, M. F., Nunes, O. C. & Manaia, C. M.(2007).Gulbenkiania mobilis gen. nov., sp. nov., isolated from treated municipal wastewater. Int J Syst Evol Microbiol 57, 1108–1112.[CrossRef] [Google Scholar]
  24. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  25. Weber, K. A., Pollock, J., Cole, K. A., O'Connor, S. M., Achenbach, L. A. & Coates, J. D.(2006). Anaerobic nitrate-dependent iron(II) bio-oxidation by a novel lithoautotrophic betaproteobacterium, strain 2002. Appl Environ Microbiol 72, 686–694.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.65755-0
Loading
Pseudogulbenkiania subflava gen. nov., sp. nov., isolated from a cold spring
Int J Syst Evol Microbiol 58, 2384 (2008); https://doi.org/10.1099/ijs.0.65755-0
/content/journal/ijsem/10.1099/ijs.0.65755-0
/content/journal/ijsem/10.1099/ijs.0.65755-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error