Strains TRF-1 and TC-9 were isolated from transfer spikes of two separate bottles containing 0.9 % NaCl (physiological saline) solution used repeatedly to wash wounds in hospital wards, months apart. Phylogenetic analysis of 16S rRNA gene sequences revealed that strains TRF-1 and TC-9 formed a distinct branch within the family most closely related to the members of the genus . The two strains, with identical 16S rRNA gene sequences, showed sequence similarities of 89.8–93.9 % with species of , 91.1–91.8 % with species of and 89.1–89.7 % with species of . Strains TRF-1 and TC-9 are Gram-positive, non-spore-forming and non-motile cocci, with an optimum growth temperature of about 37 °C. Strain TRF-1 grew optimally in medium containing 3 % (w/v) NaCl (maximum about 14 % NaCl), while strain TC-9 grew optimally in medium with 1 % (w/v) NaCl. Both strains produce a brown pigment when grown in the presence of NaCl. The cell-wall peptidoglycan is of the A3 type with a cross-linkage containing the peptide -Lys–Gly-Ser–Gly. The major respiratory quinones are menaquinone 7 (MK-7) and menaquinone 8 (MK-8), the major fatty acids are straight-chain C and C (more than 85 % of the total) and the major polar lipid is an unknown aminophospholipid. The DNA G+C content is 33.5 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, we are of the opinion that strains TRF-1 and TC-9 represent a novel species of a new genus, for which we propose the name gen. nov., sp. nov. The type strain of is strain TRF-1 (=LMG 24060 =CIP 109506).


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vol. , part 12, pp. 2939 - 2944

16S rRNA gene sequence-based phylogenies showing the relationships of strains TRF-1 and TC-9 and the closest related taxa based on the maximum-likelihood (Fig. S1) and maximum-parsimony (Fig. S2) methods. [PDF](25 KB)


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