RT Journal Article SR Electronic(1) A1 Takii, Susumu A1 Hanada, Satoshi A1 Hase, Yumiko A1 Tamaki, Hideyuki A1 Uyeno, Yutaka A1 Sekiguchi, Yuji A1 Matsuura, KatsumiYR 2008 T1 Desulfovibrio marinisediminis sp. nov., a novel sulfate-reducing bacterium isolated from coastal marine sediment via enrichment with Casamino acids JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 10 SP 2433 OP 2438 DO https://doi.org/10.1099/ijs.0.65750-0 PB Microbiology Society, SN 1466-5034, AB To obtain amino acid-utilizing sulfate reducers, enrichment culture was carried out with a medium containing Casamino acids and sulfate and inoculated with coastal marine sediment from the eutrophic Tokyo Bay, Japan. A sulfate reducer, designated strain C/L2T, was isolated from the sulfide-producing enrichment culture after further enrichment with lactate and sulfate by means of the agar shake dilution method. Cells of strain C/L2T were vibrio-shaped, Gram-negative, motile rods (0.7–1.0 μm wide and 1.0–3.5 μm long) with single polar flagella. The optimum temperature for its growth was 37 °C, the optimum pH was around 7.5 and the optimum NaCl concentration was 20–25 g l−1. Hydrogen, formate, lactate, pyruvate, fumarate, malate, succinate, ethanol, propanol, glycerol, glycine, alanine, serine, aspartate, Casamino acids, peptone and yeast extract were used as electron donors. Sulfate, sulfite and thiosulfate each served as an electron acceptor, but elemental sulfur, nitrate, fumarate, acrylate and 2,4,6-tribromophenol did not. Disproportionation of thiosulfate was not observed. Desulfoviridin, c-type cytochromes and catalase were present. The major respiratory quinone was MK-6(H2). The G+C content of the genomic DNA was 46.2 mol%. Comparisons based on 16S rRNA gene sequences and on dissimilatory sulfite reductase gene sequences clearly showed that strain C/L2T belonged to the genus Desulfovibrio: its closest relatives were the uncharacterized Desulfovibrio sp. strain TBP-1 (16S rRNA gene sequence similarity of 99.4 %) and Desulfovibrio acrylicus DSM 10141T (16S rRNA gene sequence similarity of 98.7 %). The level of DNA–DNA hybridization with Desulfovibrio acrylicus DSM 10141T was 10.3 %. On the basis of the data from this study and the physiological and phylogenetic differences that exist between the isolate and Desulfovibrio acrylicus, strain C/L2T represents a novel species of the genus Desulfovibrio, for which the name Desulfovibrio marinisediminis sp. nov. is proposed. The type strain is C/L2T (=NMRC 101113T=JCM 14577T=DSM 17456T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65750-0