To obtain amino acid-utilizing sulfate reducers, enrichment culture was carried out with a medium containing Casamino acids and sulfate and inoculated with coastal marine sediment from the eutrophic Tokyo Bay, Japan. A sulfate reducer, designated strain C/L2, was isolated from the sulfide-producing enrichment culture after further enrichment with lactate and sulfate by means of the agar shake dilution method. Cells of strain C/L2 were vibrio-shaped, Gram-negative, motile rods (0.7–1.0 μm wide and 1.0–3.5 μm long) with single polar flagella. The optimum temperature for its growth was 37 °C, the optimum pH was around 7.5 and the optimum NaCl concentration was 20–25 g l. Hydrogen, formate, lactate, pyruvate, fumarate, malate, succinate, ethanol, propanol, glycerol, glycine, alanine, serine, aspartate, Casamino acids, peptone and yeast extract were used as electron donors. Sulfate, sulfite and thiosulfate each served as an electron acceptor, but elemental sulfur, nitrate, fumarate, acrylate and 2,4,6-tribromophenol did not. Disproportionation of thiosulfate was not observed. Desulfoviridin, -type cytochromes and catalase were present. The major respiratory quinone was MK-6(H). The G+C content of the genomic DNA was 46.2 mol%. Comparisons based on 16S rRNA gene sequences and on dissimilatory sulfite reductase gene sequences clearly showed that strain C/L2 belonged to the genus : its closest relatives were the uncharacterized sp. strain TBP-1 (16S rRNA gene sequence similarity of 99.4 %) and DSM 10141 (16S rRNA gene sequence similarity of 98.7 %). The level of DNA–DNA hybridization with DSM 10141 was 10.3 %. On the basis of the data from this study and the physiological and phylogenetic differences that exist between the isolate and , strain C/L2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C/L2 (=NMRC 101113=JCM 14577=DSM 17456).


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Phase-contrast photomicrograph of cells of strain C/L2 ( sp. nov.). [PDF](723 KB)


Phylogenetic tree based on gene sequences. [PDF](28 KB)


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