1887

Abstract

A Gram-negative, moderately halophilic, short rod-shaped, aerobic bacterium with peritrichous flagellae, strain DQD2-30, was isolated from a soil sample contaminated with crude oil from the Daqing oilfield in Heilongjiang Province, north-eastern China. The novel strain was capable of growth at NaCl concentrations of 1–15 % (w/v) [optimum at 5–10 % (w/v)]. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain belonged to the genus in the class ; the highest 16S rRNA gene sequence similarities were with DSM 9502 (98.8 %), A4 (96.6 %) and CGMCC 1.6133 (95.1 %). The major cellular fatty acids of strain DQD2-30 were C 7 (43.97 %), Ccyclo 8 (23.37 %) and C (14.83 %). The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q9). The DNA G+C content was 67.0 mol%. The DNA–DNA hybridization values of strain DQD2-30 with the most closely related species of the genus were 51.8 %, 28.4 % and 23.5 % for , and , respectively. Based on these analyses, strain DQD2-30(=CGMCC 1.6443=LMG 23896) is proposed to represent the type strain of a novel species, sp. nov.

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2008-12-01
2021-10-26
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