1887

Abstract

A Gram-negative, moderately halophilic, short rod-shaped, aerobic bacterium with peritrichous flagellae, strain DQD2-30, was isolated from a soil sample contaminated with crude oil from the Daqing oilfield in Heilongjiang Province, north-eastern China. The novel strain was capable of growth at NaCl concentrations of 1–15 % (w/v) [optimum at 5–10 % (w/v)]. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain belonged to the genus in the class ; the highest 16S rRNA gene sequence similarities were with DSM 9502 (98.8 %), A4 (96.6 %) and CGMCC 1.6133 (95.1 %). The major cellular fatty acids of strain DQD2-30 were C 7 (43.97 %), Ccyclo 8 (23.37 %) and C (14.83 %). The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q9). The DNA G+C content was 67.0 mol%. The DNA–DNA hybridization values of strain DQD2-30 with the most closely related species of the genus were 51.8 %, 28.4 % and 23.5 % for , and , respectively. Based on these analyses, strain DQD2-30(=CGMCC 1.6443=LMG 23896) is proposed to represent the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65746-0
2008-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/12/2859.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65746-0&mimeType=html&fmt=ahah

References

  1. Barritt, M. M.(1936). The intensification of the Voges-Proskauer reaction by the addition of α-naphthol. J Pathol Bacteriol 42, 441–454.[CrossRef] [Google Scholar]
  2. Berendes, F., Gottschalk, G., Heine-Dobbernack, E., Moore, E. R. B. & Tindall, B. J.(1996).Halomonas desiderata sp. nov., a new alkaliphilic, halotolerant and denitrifying bacterium isolated from a municipal sewage works. Syst Appl Microbiol 19, 158–167.[CrossRef] [Google Scholar]
  3. Bouchotroch, S., Quesada, E., Del Moral, A., Llamas, I. & Béjar, V.(2001).Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 51, 1625–1632.[CrossRef] [Google Scholar]
  4. Clarke, P. H.(1953). Hydrogen sulphide production by bacteria. J Gen Microbiol 8, 397–407.[CrossRef] [Google Scholar]
  5. Collins, M. D., Goodfellow, M. & Minnikin, D. E.(1980). Fatty acid isoprenoid quinine and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 118, 29–37. [Google Scholar]
  6. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  7. Dobson, S. J. & Franzmann, P. D.(1996). Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int J Syst Bacteriol 46, 550–558.[CrossRef] [Google Scholar]
  8. Dong, X.-Z. & Cai, M.-Y.(2001).Manual of Determinative Bacteriology, pp. 370. Peking: Science Publishing Press.
  9. Eguchi, M., Nishikawa, T. & Macdonald, K.(1996). Responses to stress and nutrient availability by the marine ultramicrobacterium Sphingomonas sp. strain RB2256. Appl Environ Microbiol 62, 1287–1294. [Google Scholar]
  10. Embley, T. M.(1991). The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 13, 171–174.[CrossRef] [Google Scholar]
  11. Franzmann, P. D. & Tindall, B. J.(1990). A chemotaxonomic study of members of the family Halomonadaceae. Syst Appl Microbiol 13, 142–147.[CrossRef] [Google Scholar]
  12. García, M. T., Mellado, E., Ostos, J. C. & Ventosa, A.(2004).Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. Int J Syst Evol Microbiol 54, 1723–1728.[CrossRef] [Google Scholar]
  13. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  14. Johnson, J. L.(1994). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–681. Edited by P. E. Gerhardt, R. G. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology Press.
  15. Kates, M.(1986).Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier.
  16. Kaye, J. Z., Márquez, M. C., Ventosa, A. & Baross, J. A.(2004).Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int J Syst Evol Microbiol 54, 499–511.[CrossRef] [Google Scholar]
  17. Komagata, K. & Suzuki, K.(1987). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  18. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment briefings. Bioinformatics 5, 150–163. [Google Scholar]
  19. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  20. Martínez-Cánovas, M. J., Quesada, E., Llamas, I. & Béjar, V.(2004a).Halomonas ventosa sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 54, 733–737.[CrossRef] [Google Scholar]
  21. Martínez-Cánovas, M. J., Béjar, V., Martínez-Checa, F. & Quesada, E.(2004b).Halomonas anticariensis sp. nov., from Fuente de Piedra, a saline-wetland wildfowl reserve in Málaga, southern Spain. Int J Syst Evol Microbiol 54, 1329–1332.[CrossRef] [Google Scholar]
  22. Martínez-Cánovas, M. J., Quesada, E., Martínez-Checa, F. & Béjar, V.(2004c). A taxonomic study to establish the relationship between exopolysaccharide-producing bacterial strains living in diverse hypersaline habitats. Curr Microbiol 48, 348–353.[CrossRef] [Google Scholar]
  23. Martínez-Checa, F., Béjar, V., Martínez-Cánovas, M. J., Llamas, I. & Quesada, E.(2005).Halomonas almeriensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium from Cabo de Gata, Almería, south-east Spain. Int J Syst Evol Microbiol 55, 2007–2011.[CrossRef] [Google Scholar]
  24. Mata, J. A., Martínez-Cánovas, J., Quesada, E. & Béjar, V.(2002). A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef] [Google Scholar]
  25. Mormile, M. R., Romine, M. F., Garcia, M. T., Ventosa, A., Bailey, T. J. & Peyton, B. M.(1999).Halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium. Syst Appl Microbiol 22, 551–558.[CrossRef] [Google Scholar]
  26. Romano, I., Nicolaus, B., Lama, L., Manca, M. C. & Gambacorta, A.(1996). Characterization of a haloalkalophilic strictly aerobic bacterium, isolated from Pantelleria Island. Syst Appl Microbiol 19, 326–333.[CrossRef] [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  28. Vreeland, R. H., Litchfield, C. D., Martin, E. L. & Elliot, E.(1980).Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30, 485–495.[CrossRef] [Google Scholar]
  29. Wang, Y.-N., Cai, H., Yu, S.-L., Wang, Z.-Y. & Wu, X.-L.(2007).Halomonas gudaonensis sp. nov., isolated from a saline soil contaminated by crude oil. Int J Syst Evol Microbiol 57, 911–915.[CrossRef] [Google Scholar]
  30. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
  31. Zumft, W. G.(1992). The denitrifying bacteria. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Application, pp. 554–582. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer Verlag.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65746-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65746-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error