Four novel species of psychrotrophic bacteria were isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan, at a depth of 228–250 m. Cells of the five isolated strains, JAMM 0394, JAMM 0404, JAMM 0415, JAMM 0700 and JAMM 0738, were Gram-negative, rod-shaped, non-sporulating and motile by means of a single polar flagellum. The novel strains were able to produce isoprenoid quinone Q-8 as the major component. The predominant fatty acids were C and C. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strains represent a separate lineage within the genus . The G+C contents of the novel strains were 38.8–42.8 mol%. DNA–DNA hybridization values between these strains and reference strains of the genus were significantly lower than that generally accepted as the threshold for the phylogenetic definition of species. The names sp. nov. (type strain JAMM 0394=JCM 14783=ATCC BAA-1525), sp. nov. (type strain JAMM 0404=JCM 14784=ATCC BAA-1526), sp. nov. (two strains, type strain JAMM 0415=JCM 14785=ATCC BAA-1527) and sp. nov. (type strain JAMM 0738=JCM 14786=ATCC BAA-1528) are proposed.


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Transmission electron micrograph of negatively stained cells of the four novel species. [ PDF] 251 KB


DNA–DNA hybridization between the isolated strains and closely related species of the genus . [ PDF] 31 KB


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