sp. nov., a marine bacterium isolated from black sand Free

Abstract

A Gram-negative, facultatively anaerobic, motile, slightly curved rod-shaped bacterial strain was isolated from black sand collected from Soesoggak, Jeju island, Korea. The strain, designated J74, was able to grow in the presence of 1–7.5 % NaCl, at temperatures of 4–45 °C and at pH 5–10. Strain J74 was oxidase- and catalase-positive, arginine dihydrolase-negative and sensitive to the vibriostatic agent O/129. Strain J74 was characterized based on physiology, morphology, biochemical features and 16S rRNA gene sequence analysis. The isolate required sodium ions for growth and utilized a wide range of compounds as sole sources of carbon and energy. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J74 belongs to the class . It was found to be associated with the genus and was phylogenetically related most closely to the type strain of (98.7 % 16S rRNA gene sequence similarity). However, DNA–DNA hybridization experiments between strain J74 and KCTC 12827 revealed a level of relatedness of 37.7 %. Thus, phenotypic and phylogenetic data suggested that J74 should be placed in the genus as representing a novel species, for which the name sp. nov. is proposed. The type strain is J74 (=KCTC 22122 =JCM 14949).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65726-0
2008-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/8/1903.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65726-0&mimeType=html&fmt=ahah

References

  1. Austin, B.(1988).Marine Microbiology. Cambridge: Cambridge University Press.
  2. Chun, J., Lee, J. H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  4. Gonzalez, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B.(1997).Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47, 369–376.[CrossRef] [Google Scholar]
  5. Gordon, R. E., Haynes, W. C. & Pang, C. H.-N.(1973).The Genus Bacillus, US Department of Agriculture Handbook no. 427. Washington, DC: US Department of Agriculture.
  6. Hjeltnes, B. & Roberts, R. J.(1993). Vibriosis. In Bacterial Diseases of Fish, pp. 109–121. Edited by V. Inglis, R. J. Roberts & N. R. Bromage. Oxford: Blackwell Scientific.
  7. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  8. Kushmaro, A., Banin, E., Loya, Y., Stackebrandt, E. & Rosenberg, E.(2001).Vibrio shiloi sp. nov., the causative agent of bleaching of the coral Oculina patagonica. Int J Syst Evol Microbiol 51, 1383–1388. [Google Scholar]
  9. Lambert, C., Nicolas, J. L., Cilia, V. & Corre, S.(1998).Vibrio pectenicida sp. nov., a pathogen of scallop (Pecten maximus) larvae. Int J Syst Bacteriol 48, 481–487.[CrossRef] [Google Scholar]
  10. Lightner, D. V.(1993). Diseases of cultured penaeid shrimp. In CRC Handbook of Mariculture, 2nd edn, vol. 1, pp. 393–486. Edited by J. P. McVey. Boca Raton, FL: CRC Press.
  11. Pacini, F.(1854). Osservazione microscopiche e deduzioni patologiche sul cholera asiatico. Gaz Med Ital Toscano Firenze 6, 405–412 (in Italian). [Google Scholar]
  12. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  13. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  14. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  15. Stackebrandt, E., Liesack, W. & Goebel, B. M.(1993). Bacterial diversity in a soil sample from a subtropical Australian environment as determined by 16S rDNA analysis. FASEB J 7, 232–236. [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  17. Thompson, F. L., Li, Y., Gomez-Gil, B., Thompson, C. C., Hoste, B., Vandemeulebroecke, K., Rupp, G. S., Pereira, A., De Bem, M. M. & other authors(2003).Vibrio neptunius sp. nov., Vibrio brasiliensis sp. nov. and Vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps). Int J Syst Evol Microbiol 53, 245–252.[CrossRef] [Google Scholar]
  18. Thompson, F. L., Iida, T. & Swings, J.(2004). Biodiversity of vibrios. Microbiol Mol Biol Rev 68, 403–431.[CrossRef] [Google Scholar]
  19. Yoon, J. H., Lee, S. T. & Park, Y. H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65726-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65726-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed