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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 8

sp. nov., a marine bacterium isolated from black sand Free

Abstract

A Gram-negative, motile, rod-shaped bacterium was isolated from black sand collected at Soesoggak, Jeju island, Korea. The strain, designated J83, was able to grow in the presence of 5 % NaCl, at temperatures of 4–45 °C and over the pH range 5.5–9.5. The isolate reduced nitrate to nitrite and was positive for oxidase, catalase, alkaline phosphatase and leucine arylamidase. Strain J83 utilized malate, maltose, mannitol and glucose as sole sources of carbon. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain J83 belongs to the class and is related to species of the genus . Strain J83 exhibited 97.8 % 16S rRNA gene sequence similarity to the type strain of . Based on DNA–DNA hybridization, the level of relatedness between strain J83 and NBRC 100975 was 39.3 %. On the basis of phenotypic, genetic and phylogenetic data, strain J83 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J83 (=KCTC 22121 =JCM 14937).

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2008-08-01
2023-09-29
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References

  1. Baumann, P. & Baumann, L.(1981). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  2. Bowman, J. P., McCammon, S. A., Nichols, D. S., Skerratt, J. H., Rea, S. M., Nichols, P. D. & McMeekin, T. A.(1997).Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20 : 5ω3) and grow anaerobically by dissimilatory Fe(III) reduction. Int J Syst Bacteriol 47, 1040–1047.[CrossRef] [Google Scholar]
  3. Bozal, N., Montes, M. J., Tudela, E., Jimenez, F. & Guinea, J.(2002).Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52, 195–205. [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  5. Gonzalez, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B.(1997).Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47, 369–376.[CrossRef] [Google Scholar]
  6. Gordon, R. E., Haynes, W. C. & Pang, C. H.-N.(1973).The Genus Bacillus, US Department of Agriculture Handbook no. 427. Washington, DC: Agricultural Research Service.
  7. Ivanova, E. P., Flavier, S. & Christen, R.(2004). Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54, 1773–1788.[CrossRef] [Google Scholar]
  8. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  9. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  10. MacDonell, M. T. & Colwell, R. R.(1985). Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella. Syst Appl Microbiol 6, 171–182.[CrossRef] [Google Scholar]
  11. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  12. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  13. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  14. Satomi, M., Vogel, B. F., Gram, L. & Venkateswaran, K.(2006).Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea. Int J Syst Evol Microbiol 56, 243–249.[CrossRef] [Google Scholar]
  15. Satomi, M., Vogel, B. F., Venkateswaran, K. & Gram, L.(2007). Description of Shewanella glacialipiscicola sp. nov. and Shewanella algidipiscicola sp. nov., isolated from marine fish of the Danish Baltic Sea, and proposal that Shewanella affinis is a later heterotypic synonym of Shewanella colwelliana. Int J Syst Evol Microbiol 57, 347–352.[CrossRef] [Google Scholar]
  16. Skerratt, J. H., Bowman, J. P. & Nichols, P. D.(2002).Shewanella olleyana sp. nov., a marine species isolated from a temperate estuary which produces high levels of polyunsaturated fatty acids. Int J Syst Evol Microbiol 52, 2101–2106.[CrossRef] [Google Scholar]
  17. Stackebrandt, E., Liesack, W. & Goebel, B. M.(1993). Bacterial diversity in a soil sample from a subtropical Australian environment as determined by 16S rDNA analysis. FASEB J 7, 232–236. [Google Scholar]
  18. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  19. Venkateswaran, K., Dollhopf, M. E., Aller, R., Stackebrandt, E. & Nealson, K. H.(1998).Shewanella amazonensis sp. nov., a novel metal-reducing facultative anaerobe from Amazonian shelf muds. Int J Syst Bacteriol 48, 965–972.[CrossRef] [Google Scholar]
  20. Venkateswaran, K., Moser, D. P., Dollhopf, M. E., Lies, D. P., Saffarini, D. A., MacGregor, B. J., Ringelberg, D. B., White, D. C., Nishijima, M. & other authors(1999). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49, 705–724.[CrossRef] [Google Scholar]
  21. Xiao, X., Wang, P., Zeng, X., Bartlett, D. H. & Wang, F.(2007).Shewanella psychrophila sp. nov. and Shewanella piezotolerans sp. nov., isolated from west Pacific deep-sea sediment. Int J Syst Evol Microbiol 57, 60–65.[CrossRef] [Google Scholar]
  22. Yang, S. H., Kwon, K. K., Lee, H. S. & Kim, S. J.(2006).Shewanella spongiae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 56, 2879–2882.[CrossRef] [Google Scholar]
  23. Yang, S. H., Lee, J. H., Ryu, J. S., Kato, C. & Kim, S. J.(2007).Shewanella donghaensis sp. nov., a psychrophilic, piezosensitive bacterium producing high levels of polyunsaturated fatty acid, isolated from deep-sea sediments. Int J Syst Evol Microbiol 57, 208–212.[CrossRef] [Google Scholar]
  24. Yoon, J. H., Lee, S. T. & Park, Y. H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef] [Google Scholar]
  25. Yoon, J. H., Kang, K. H., Oh, T. K. & Park, Y. H.(2004).Shewanella gaetbuli sp. nov., a slight halophile isolated from a tidal flat in Korea. Int J Syst Evol Microbiol 54, 487–491.[CrossRef] [Google Scholar]
  26. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R.(1998). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef] [Google Scholar]
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Shewanella basaltis sp. nov., a marine bacterium isolated from black sand
Int J Syst Evol Microbiol 58, 1907 (2008); https://doi.org/10.1099/ijs.0.65725-0
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