1887

Abstract

An aerobic, yellow-pigmented, Gram-negative bacterium, designated strain BD-b365, was isolated from sediment of the Hakjang stream in Busan, South Korea. Growth was observed at 15–40 °C (optimum 20–30 °C) and at pH 6.0–9.5 (optimum pH 7.0–8.0). Cells were non-spore-forming rods that showed gliding motility and contained branched and hydroxy fatty acids. The G+C content of the genomic DNA was 35.4 mol%. The major respiratory quinone was menaquinone-6 (MK-6). The major polar lipid of strain BD-b365 was phosphatidylethanolamine. Comparative 16S rRNA gene sequence analysis showed that strain BD-b365 formed a distinct phyletic line within the genus . Based on levels of 16S rRNA gene sequence similarity, the novel strain was related most closely to WB 1.1-56, but the level of DNA–DNA relatedness between these two strains was only 9.6 %. On the basis of phenotypic and genotypic data, it is clear that strain BD-b365 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BD-b365 (=KCTC 22078 =DSM 19382).

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2008-10-01
2020-01-26
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Neighbour-joining tree based on 16S rRNA gene sequences showing the phylogenetic relationships of strain BD-b365 and the type strains of all species with validly published names. [PDF](21 KB)

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Transmission electron micrograph showing the general morphology of negatively stained cells of strain BD-b365 after growth for 2 days at 30 °C on R2A agar.

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Cellular fatty acid compositions of strain BD-b365 and type strains of related species. [PDF](79 KB)

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