1887

Abstract

A moderately thermophilic, nitrate-reducing bacterium, strain Yu37-1, was isolated from hot spring water from Yumata, Nagano, Japan. Cells of strain Yu37-1 were strictly anaerobic, Gram-negative, non-sporulating, motile by means of a single polar flagellum, vibrio-shaped and 1.4–2.0 μm long. The temperature and pH for optimum growth were 55 °C and pH 7.0–7.5, respectively. Strain Yu37-1 grew best in basal medium without the addition of NaCl. Acetate, pyruvate, lactate, fumarate, succinate, malate, yeast extract, peptone and Casamino acids were utilized as electron donors, with nitrate as the only electron acceptor. Ammonium was the end product from nitrate. The G+C content of the genomic DNA was 35.1 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that strain Yu37-1 could be accommodated in the family and that its closest neighbours were members of the five genera of the family , namely , , , and , with similarities of only 83.2–86.2 %. The growth temperature and salinity range for growth of strain Yu37-1 differed from those of the phylogenetically related organisms. On the basis of phenotypic features and phylogenetic position, a novel genus and species are proposed, gen. nov., sp. nov. Strain Yu37-1 (=NBRC 101217 =DSM 19672) is the type strain of .

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2008-07-01
2019-09-22
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References

  1. Adachi, J. & Hasegawa, M. ( 1995; ). Improved dating of the human/chimpanzee separation in the mitochondrial DNA tree: heterogeneity among amino acid sites. J Mol Evol 40, 622–628.[CrossRef]
    [Google Scholar]
  2. Barns, S. M., Fundyga, R. E., Jeffries, M. W. & Pace, N. R. ( 1994; ). Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment. Proc Natl Acad Sci U S A 91, 1609–1613.[CrossRef]
    [Google Scholar]
  3. Caccavo, F. Jr, Coates, J. D., Rossello-Mora, R. A., Ludwig, W., Schleifer, K. H., Lovley, D. R. & McInerney, M. J. ( 1996; ). Geovibrio ferrireducens, a phylogenetically distinct dissimilatory Fe(III)-reducing bacterium. Arch Microbiol 165, 370–376.[CrossRef]
    [Google Scholar]
  4. Fiala, G., Woese, C. R., Langworthy, T. A. & Stetter, K. O. ( 1990; ). Flexistipes sinusarabici, a novel genus and species of eubacteria occurring in the Atlantis II Deep brines of the Red Sea. Arch Microbiol 154, 120–126.[CrossRef]
    [Google Scholar]
  5. Greene, A. C., Patel, B. K. C. & Sheehy, A. J. ( 1997; ). Deferribacter thermophilus gen. nov., sp. nov., a novel thermophilic manganese- and iron-reducing bacterium isolated from a petroleum reservoir. Int J Syst Bacteriol 47, 505–509.[CrossRef]
    [Google Scholar]
  6. Hiraishi, A., Umezawa, T., Yamamoto, H., Kato, K. & Maki, Y. ( 1999; ). Changes in quinone profiles of hot spring microbial mats with a thermal gradient. Appl Environ Microbiol 65, 198–205.
    [Google Scholar]
  7. Hugenholtz, P., Pitulle, C., Hershberger, K. L. & Pace, N. R. ( 1998; ). Novel division level bacterial diversity in a Yellowstone hot spring. J Bacteriol 180, 366–376.
    [Google Scholar]
  8. Iino, T., Mori, K., Tanaka, K., Suzuki, K. & Harayama, S. ( 2007; ). Oscillibacter valericigenes gen. nov., sp. nov., a valerate-producing anaerobic bacterium isolated from the alimentary canal of a Japanese corbicula clam. Int J Syst Evol Microbiol 57, 1840–1845.[CrossRef]
    [Google Scholar]
  9. Itoh, T., Suzuki, K. & Nakase, T. ( 2002; ). Vulcanisaeta distributa gen. nov., sp. nov., and Vulcanisaeta souniana sp. nov., novel hyperthermophilic, rod-shaped crenarchaeotes isolated from hot springs in Japan. Int J Syst Evol Microbiol 52, 1097–1104.[CrossRef]
    [Google Scholar]
  10. Janssen, P. H., Liesack, W. & Schink, B. ( 2002; ). Geovibrio thiophilus sp. nov., a novel sulfur-reducing bacterium belonging to the phylum Deferribacteres. Int J Syst Evol Microbiol 52, 1341–1347.[CrossRef]
    [Google Scholar]
  11. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  12. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  13. Miroshnichenko, M. L., Slobodkin, A. I., Kostrikina, N. A., L'Haridon, S., Nercessian, O., Spring, S., Stackebrandt, E., Bonch-Osmolovskaya, E. A. & Jeanthon, C. ( 2003; ). Deferribacter abyssi sp. nov., an anaerobic thermophile from deep-sea hydrothermal vents of the Mid-Atlantic Ridge. Int J Syst Evol Microbiol 53, 1637–1641.[CrossRef]
    [Google Scholar]
  14. Myhr, S. & Torsvik, T. ( 2000; ). Denitrovibrio acetiphilus, a novel genus and species of dissimilatory nitrate-reducing bacterium isolated from an oil reservoir model column. Int J Syst Evol Microbiol 50, 1611–1619.[CrossRef]
    [Google Scholar]
  15. Nakagawa, T. & Fukui, M. ( 2002; ). Phylogenetic characterization of microbial mats and streamers from a Japanese alkaline hot spring with a thermal gradient. J Gen Appl Microbiol 48, 211–222.[CrossRef]
    [Google Scholar]
  16. Nakagawa, T. & Fukui, M. ( 2003; ). Molecular characterization of community structures and sulfur metabolism within microbial streamers in Japanese hot springs. Appl Environ Microbiol 69, 7044–7057.[CrossRef]
    [Google Scholar]
  17. Robertson, B. R., O'Rourke, J. L., Neilan, B. A., Vandamme, P., On, S. L. W., Fox, J. G. & Lee, A. ( 2005; ). Mucispirillum schaedleri gen. nov., sp. nov., a spiral-shaped bacterium colonizing the mucus layer of the gastrointestinal tract of laboratory rodents. Int J Syst Evol Microbiol 55, 1199–1204.[CrossRef]
    [Google Scholar]
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  20. Sekiguchi, Y., Kamagata, Y., Nakamura, K., Ohashi, A. & Harada, H. ( 2000; ). Syntrophothermus lipocalidus gen. nov., sp. nov., a novel themophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate. Int J Syst Evol Microbiol 50, 771–779.[CrossRef]
    [Google Scholar]
  21. Takai, K., Kobayashi, H., Nealson, K. H. & Horikoshi, K. ( 2003a; ). Sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer. Int J Syst Evol Microbiol 53, 823–827.[CrossRef]
    [Google Scholar]
  22. Takai, K., Kobayashi, H., Nealson, K. H. & Horikoshi, K. ( 2003b; ). Deferribacter desulfuricans sp. nov., a novel sulfur-, nitrate- and arsenate-reducing thermophile isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 53, 839–846.[CrossRef]
    [Google Scholar]
  23. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  24. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  25. Tsubota, J., Eshinimaev, B. Ts., Khmelenina, V. N. & Trotsenko, Y. A. ( 2005; ). Methylothermus thermalis gen. nov., sp. nov., a novel moderately thermophilic obligate methanotroph from a hot spring in Japan. Int J Syst Evol Microbiol 55, 1877–1884.[CrossRef]
    [Google Scholar]
  26. Ward, D. M., Weller, R. & Bateson, M. M. ( 1990; ). 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature 345, 63–65.[CrossRef]
    [Google Scholar]
  27. Wolin, E. A., Wolin, M. J. & Wolfe, R. S. ( 1963; ). Formation of methane by bacterial extracts. J Biol Chem 238, 2882–2886.
    [Google Scholar]
  28. Yamamoto, H., Hiraishi, A., Kato, K., Chiura, H. X., Maki, Y. & Shimizu, A. ( 1998; ). Phylogenetic evidence for the existence of novel thermophilic bacteria in hot spring sulfur-turf microbial mats in Japan. Appl Environ Microbiol 64, 1680–1687.
    [Google Scholar]
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