1887

Abstract

A hexachlorocyclohexane (HCH)-degrading bacterial strain (RL-3) was isolated from an HCH dump site located in the northern part of India. Resting cell assays and analytical GC studies confirmed the ability of strain RL-3 to degrade HCH isomers. Southern blot hybridization studies revealed the presence of genes, which are involved in the HCH degradation pathway, in this bacterium. The 16S rRNA gene sequence of strain RL-3 showed that it was most closely related to JCM 10874 (97.3 %) and MTCC 7295 (96.4 %). Phylogenetic analysis based on 16S rRNA gene sequences placed strain RL-3 between JCM 10874 and MTCC 7295. The DNA G+C content of strain RL-3 was 62 mol%. The DNA–DNA relatedness values of strain RL-3 with JCM 10874 and CCM 7327 were 8.65 and 7.47 %, respectively. SGL1 was the major sphingolipid and spermidine was the major polyamine in strain RL-3. The major fatty acids in strain RL-3 were C 7 (56.6 %), C (14 %) and C 2-OH (7.4 %). Ubiquinone Q-10 was the major respiratory quinone. Phylogenetic distinctiveness, DNA–DNA relatedness values, biochemical and physiological characterization and unique phenotypic characteristics suggest that strain RL-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RL-3 (=MTCC 8599=CCM 7431).

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2009-01-01
2019-10-18
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Cellular fatty acid profiles of strain RL-3 and related species. [ PDF] 25 KB

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Degradation of HCH isomers by strain RL-3 in phosphate buffer. [ PDF] 106 KB

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Two dimensional TLC of polar lipids of strain RL-3 . [ PDF] 134 KB

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