1887

Abstract

A halophilic actinomycete strain, designated YIM 90500, was isolated from a saline lake in Xinjiang province, north-west China and subjected to a polyphasic taxonomic study. Good growth of the novel isolate occurred at 28–37 °C, pH 7–8 and with 10–15 % (w/v) NaCl; no growth occurred without any salts. Strain YIM 90500 contained -diaminopimelic acid with glucose, arabinose and ribose as the whole-cell sugars. The major phospholipids were phosphatidylcholine and diphosphatidylglycerol. MK-9(H) was the predominant menaquinone. The major fatty acids were iso-C, iso-C and anteiso-C. The chemotaxonomic data together with the morphological properties of strain YIM 90500 consistently assigned the strain as belonging to the genus . Phylogenetic analysis based on 16S rRNA gene sequences further revealed that strain YIM 90500 formed a distinct phyletic lineage in the genus and showed low 16S rRNA gene similarities (<96.4 %) with other species of the genus. On the basis of the evidence from the polyphasic study, a novel species, sp. nov., is proposed. The type strain is YIM 90500 (=DSM 45007=KCTC 19162).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65705-0
2009-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/3/555.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65705-0&mimeType=html&fmt=ahah

References

  1. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  2. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  3. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  4. Goodfellow, M., Lacey, J., Athalye, M., Embley, T. M. & Bowen, T.(1989).Saccharopolyspora gregorii and Saccharopolyspora hordei: two new actinomycete species from fodder. J Gen Microbiol 135, 2125–2139. [Google Scholar]
  5. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  6. Kelly, K. L.(1964).Inter-Society Color Council-National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  7. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  8. Korn-Wendisch, F., Kempf, A., Grund, E., Kroppenstedt, R. M. & Kutzner, H. J.(1989). Transfer of Faenia rectivirgula Kurup and Agre 1983 to the genus Saccharopolyspora Lacey and Goodfellow 1975, elevation of Saccharopolyspora hirsuta subsp. taberi Labeda 1987 to species level, and emended description of the genus Saccharopolyspora. Int J Syst Bacteriol 39, 430–441.[CrossRef] [Google Scholar]
  9. Kroppenstedt, R. M.(1982). Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2387.[CrossRef] [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  11. Labeda, D. P.(1987). Transfer of the type strain of Streptomyces erythraeus (Waksman, 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. Int J Syst Bacteriol 37, 19–22.[CrossRef] [Google Scholar]
  12. Lacey, J. & Goodfellow, M.(1975). A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et sp. nov. J Gen Microbiol 88, 75–85.[CrossRef] [Google Scholar]
  13. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef] [Google Scholar]
  14. Lu, Z., Liu, Z., Wang, L., Zhang, Y., Qi, W. & Goodfellow, M.(2001).Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp. nov., novel actinomycetes from soil. Int J Syst Evol Microbiol 51, 319–325. [Google Scholar]
  15. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  16. Mertz, F. P. & Yao, R. C.(1990).Saccharopolyspora spinosa sp. nov., isolated from soil collected in a sugar mill rum still. Int J Syst Bacteriol 40, 34–39.[CrossRef] [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  18. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  19. Pimentel-Elardo, S. M., Tiro, L. P., Grozdanov, L. & Hentsche, U.(2008).Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera). Int J Syst Evol Microbiol 58, 628–632.[CrossRef] [Google Scholar]
  20. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  21. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  22. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  24. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
  25. Williams, S. T., Goodfellow, M. & Alderson, G.(1989). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  26. Yuan, L.-J., Zhang, Y.-Q., Guan, Y., Wei, Y.-Z., Li, Q. P., Yu, L.-Y., Li, W.-J. & Zhang, Y.-Q.(2008).Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. Int J Syst Evol Microbiol 58, 1180–1185.[CrossRef] [Google Scholar]
  27. Zhang, J., Wu, D., Zhang, J., Liu, Z. & Song, F.(2008).Saccharopolyspora shandongensis sp. nov., isolated from wheat-field soil. Int J Syst Evol Microbiol 58, 1094–1099.[CrossRef] [Google Scholar]
  28. Zhou, Z. H., Liu, Z. H., Qian, Y. D., Kim, S. B. & Goodfellow, M.(1998).Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48, 53–58.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65705-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65705-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 555 - 558

Scanning electron micrograph of spore chains of strain YIM 90500 ( sp. nov.) [PDF](258 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error