1887

Abstract

A novel halophilic archaeon, strain CGSA15, was isolated from water of Lake Chagannor in China. The strain grew optimally at 33–37 °C, pH 9.5–10.0 and 4.0–4.3 M NaCl. The major polar lipids were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. The genomic DNA G+C content of strain CGSA15 was 60.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CGSA15 was a member of the genus and was related most closely to AS 1.3528 (96.1 % similarity) and AS 1.3239 (96.9 %). Levels of DNA–DNA relatedness between strain CGSA15 and AS 1.3528 and AS 1.3239 were 36.7 and 28.9 %, respectively. According to the phenotypic and genotypic data presented, strain CGSA15 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CGSA15 (=CGMCC 1.6783 =CECT 7303).

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2008-07-01
2020-01-18
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Micrographs of cells of strain CGSA15 in liquid medium under optimal conditions. (top) Phase-contrast micrograph showing pleomorphic forms. Bar, 5 µm. (bottom) Electron micrographs of uranyl acetate-stained cells showing flagella. Bars, 0.5 µm.

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Polar lipid pattern of strain CGSA15 by two-dimensional TLC. PG, Phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester. Direction of development: first dimension, horizontal; second dimension, vertical. The origin is at the bottom-left corner.

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