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Volume 58, Issue 10

sp. nov., a moderately halophilic bacterium isolated from saline soil Free

Abstract

A Gram-positive, moderately halophilic, moderately thermophilic, endospore-forming, catalase- and oxidase-positive, obligately aerobic bacterium, strain YIM-C55.5, was isolated from saline soil in the Qaidam Basin, north-west China. Cells were slender rods, motile by means of peritrichous flagella. Filamentous forms were present throughout the growth cycle. Strain YIM-C55.5 was able to grow in the presence of 7–30 % (w/v) NaCl and at pH 6.0–9.0, with optimum growth at 15 % (w/v) NaCl and pH 7.0. It was able to grow at 28–60 °C, with optimum growth at 45–50 °C. -Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C, C, anteiso-C, anteiso-C and C. Strain YIM-C55.5 possessed menaquinone 7 (MK-7) as the predominant respiratory quinone and diphosphatidylglycerol and phosphatidylglycerol as polar lipids. The genomic DNA G+C content was 42.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM-C55.5 belonged to the family and was related most closely to members of the genus , but with low levels of similarity to the type strains of recognized species of the genus ( XH-63, 95.1 %; T-16X, 94.0 %; DD1, 93.7 %; NN, 93.5 %). Together with phenotypic data, these results suggest that strain YIM-C55.5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM-C55.5 (=DSM 17856 =CGMCC 1.6303).

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2008-10-01
2024-04-24
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References

  1. Ahmed, I., Yokota, A. & Fujiwara, T.(2007).Gracilibacillus boraciitolerans sp. nov., a highly boron-tolerant and moderately halotolerant bacterium isolated from soil. Int J Syst Evol Microbiol 57, 796–802.[CrossRef] [Google Scholar]
  2. Atlas, R. M.(1993).Handbook of Microbiological Media. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  3. Carrasco, I. J., Márquez, M. C., Xue, Y., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A.(2006).Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. Int J Syst Evol Microbiol 56, 599–604.[CrossRef] [Google Scholar]
  4. Chen, Y.-G., Cui, X.-L., Pukall, R., Li, H.-M., Yang, Y.-L., Xu, L.-H., Wen, M.-L., Peng, Q. & Jiang, C.-L.(2007).Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57, 2327–2332.[CrossRef] [Google Scholar]
  5. Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef] [Google Scholar]
  6. Cui, X.-L., Mao, P.-H., Zeng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L.(2001).Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363. [Google Scholar]
  7. Deutch, C. E.(1994). Characterization of a novel salt-tolerant Bacillus sp. from the nasal cavities of desert iguanas. FEMS Microbiol Lett 121, 55–60.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  9. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Garabito, M. J., Arahal, D. R., Mellado, E., Márquez, M. C. & Ventosa, A.(1997).Bacillus salexigens sp. nov., a new moderately halophilic Bacillus species. Int J Syst Bacteriol 47, 735–741.[CrossRef] [Google Scholar]
  12. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Krieg, N. R. & Philips, G. B.(1981).Manual of Methods for General Bacteriology. Washington, DC: American Society for Microbiology.
  13. Gregersen, T.(1978). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef] [Google Scholar]
  14. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  15. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  16. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P. & Ward, J. M.(1985). Preparation of chromosomal, plasmid and phage DNA. In Genetic Manipulation of Streptomyces: a Laboratory Manual, pp. 79–80. Norwich, UK: John Innes Foundation.
  17. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  18. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  19. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  20. Kushner, D. J.(1993). Growth and nutrition of halophilic bacteria. In The Biology of Halophilic Bacteria, pp. 87–103. Edited by R. H. Vreeland & L. I. Hochstein. Boca Raton, FL: CRC Press.
  21. Lawson, P. A., Deutch, C. E. & Collins, M. D.(1996). Phylogenetic characterization of a novel salt-tolerant Bacillus species: description of Bacillus dipsosauri sp. nov. J Appl Bacteriol 81, 109–112.[CrossRef] [Google Scholar]
  22. Mandel, M. & Marmur, J.(1968). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206. [Google Scholar]
  23. Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef] [Google Scholar]
  24. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  25. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  26. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R.(1981). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  28. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  29. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  30. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A.(1982). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968. [Google Scholar]
  31. Wainø, M., Tindall, B. J., Schumann, P. & Ingvorsen, K.(1999).Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49, 821–831.[CrossRef] [Google Scholar]
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Gracilibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from saline soil
Int J Syst Evol Microbiol 58, 2403 (2008); https://doi.org/10.1099/ijs.0.65698-0
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