A strain, designated 203, previously isolated from Bobal grape must was characterized phylogenetically, genotypically and phenotypically in order to establish whether it represents a novel species. On the basis of the 16S rRNA gene sequence, strain 203 was shown to belong to the genus , falling within the group and being closely related to the type strains of , and . DNA–DNA hybridization results confirmed the separate status of strain 203 at the species level. To establish the similarities and differences between 203 and the three aforementioned closest species, the following methods were used: amplified rDNA restriction analysis, analysis of the 16S–23S rDNA intergenic spacer region, random amplification of polymorphic DNA (RAPD) profiling, ribotyping, carbohydrate fermentation and physiological tests. Strain 203 could be differentiated genetically using RAPD analysis and ribotyping. Phenotypically, it can be distinguished from its closest relatives by its ability to grow at pH 3.3, by gas production from gluconate and by certain carbohydrate fermentations. On the basis of these data, strain 203 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 203 (=CECT 7310 =DSM 19674).


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vol. , part 12, pp. 2699 - 2703

Phylogenetic trees based on 16S rRNA gene sequences constructed using the maximum-parsimony method (a) and the neighbour-joining method (b). [PDF](30 KB)

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