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International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 10

sp. nov., a halophilic bacterium isolated from a Saharan salt lake Free

Abstract

A Gram-positive, endospore-forming, rod-shaped and moderately halophilic bacterium was isolated from a salt-crust sample collected from Gharsa salt lake (Chott el Gharsa), Tunisia. The newly isolated bacterium, designated SA-Vb1, was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Strain SA-Vb1 was closely related to the type strains of and , with 16S rRNA gene sequence similarities of 99.7 and 99.4 %, respectively. However, strain SA-Vb1 was distinguished from these two type strains on the basis of phenotypic characteristics and DNA–DNA relatedness (29.4 and 5.1 %, respectively). The genetic relationship between strain SA-Vb1 and IAM 11061 (the type strain of the type species) and other type strains of the genus was 96–98 % based on 16S rRNA gene sequence similarity and 18.3–22.3 % based on DNA–DNA hybridization. Biochemical analysis resulted in determination of major fatty acids iso-C, anteiso-C and anteiso-C (33.3, 29.2 and 9.8 %, respectively); phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine were the main polar lipids and MK-7 was the predominant menaquinone (∼100 %). The distinct characteristics demonstrated by strain SA-Vb1 represent properties of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SA-Vb1 (=JCM 12946 =DSM 18441).

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Keyword(s): CZE, capillary zone electrophoresis
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2008-10-01
2020-01-19
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Virgibacillus salarius sp. nov., a halophilic bacterium isolated from a Saharan salt lake
Int J Syst Evol Microbiol 58, 2409 (2008); https://doi.org/10.1099/ijs.0.65693-0
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vol. , part 10, pp. 2409 - 2414

Cellular fatty acid compositions of strain SA-Vb1 and type strains of related species.

16S rRNA gene sequence similarity and DNA–DNA relatedness of strain SA-Vb1 and type strains of phylogenetically related species.

[PDF file of Supplementary Tables S1 and S2](68 KB)

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